Stairs surface.

<p>Fungal mycelia developing on the wood and on the old dark deposits on the surface of the stairs (<b>A</b>). Evolution of salt crusts due to the storing conditions promoting local variations in the salt content on the stairs (<b>B</b>).</p

Clustering of bacterial and archaeal strains according to RAPD-PCR and phylogenetic classification of the isolated bacteria and archaea.

<p>Clustering of bacterial and archaeal strains according to RAPD-PCR and phylogenetic classification of the isolated bacteria and archaea.</p

Phylogenetic affinities of the ITS coding sequences amplified from pure fungal strains isolated from the staircase of Hallstatt.

<p>Phylogenetic affinities of the ITS coding sequences amplified from pure fungal strains isolated from the staircase of Hallstatt.</p

The staircase of Hallstatt.

<p>The 3100 years old staircase is 8 m long and 1.20 wide, and is representative of the construction typical of the Bronze Age (C: NHM/A. Rausch) (<b>A</b>). After its discovery, a wooden shelter was built over the staircase to ensure its preservation (C: NHM/A. Rausch) (<b>B</b>).</p

MOESM2 of Bronze Age meat industry: ancient mitochondrial DNA analyses of pig bones from the prehistoric salt mines of Hallstatt (Austria)

Additional file 2. Map of the porcine mitochondrial DNA. Localisation of the 721 bp long control region (CR) fragment in the reference mitochondrial genome

MOESM6 of Bronze Age meat industry: ancient mitochondrial DNA analyses of pig bones from the prehistoric salt mines of Hallstatt (Austria)

Additional file 6. Phylogenetic Reconstruction I—estimates of evolutionary divergence between sequences. Distance matrix shows the number of base differences per site (p distances; Nei and Kumar 2000) between 42 DNA sequences (length of alignment 637 bp)

MOESM1 of Bronze Age meat industry: ancient mitochondrial DNA analyses of pig bones from the prehistoric salt mines of Hallstatt (Austria)

Additional file 1. Ancient DNA extraction and Mitochondrial control region PCR. Detailed protocols for Ancient DNA extraction and PCR of mitochondrial control region

MOESM3 of Bronze Age meat industry: ancient mitochondrial DNA analyses of pig bones from the prehistoric salt mines of Hallstatt (Austria)

Additional file 3. PCR strategy for the 721-bp-long section of the mitochondrial Control Region (CR). The CR sequences were inferred using three PCR primer pairs that allow amplification of overlapping amplicons, ranging from 343 bp to 401 bp in length

MOESM9 of Bronze Age meat industry: ancient mitochondrial DNA analyses of pig bones from the prehistoric salt mines of Hallstatt (Austria)

Additional file 9. Supplementary references. Publications being cited in Additional files 1–9

MOESM7 of Bronze Age meat industry: ancient mitochondrial DNA analyses of pig bones from the prehistoric salt mines of Hallstatt (Austria)

Additional file 7. Phylogenetic Reconstruction II—Maximum Likelihood fits of 24 different nucleotide substitution models. Summarizing table for the models with the lowest BIC scores (Bayesian Information Criterion) that are considered to describe the substitution pattern the best