21 research outputs found
Microfluidic, Label-Free Enrichment of Prostate Cancer Cells in Blood Based on Acoustophoresis
Circulating tumor cells (CTC) are shed in peripheral
blood at advanced
metastatic stages of solid cancers. Surface-marker-based detection
of CTC predicts recurrence and survival in colorectal, breast, and
prostate cancer. However, scarcity and variation in size, morphology,
expression profile, and antigen exposure impairs reliable detection
and characterization of CTC. We have developed a noncontact, label-free
microfluidic acoustophoresis method to separate prostate cancer cells
from white blood cells (WBC) through forces generated by ultrasonic
resonances in microfluidic channels. Implementation of cell prealignment
in a temperature-stabilized (±0.5 °C) acoustophoresis microchannel
dramatically enhanced the discriminatory capacity and enabled the
separation of 5 μm microspheres from 7 μm microspheres
with 99% purity. Next, we determined the feasibility of employing
label-free microfluidic acoustophoresis to discriminate and divert
tumor cells from WBCs using erythrocyte-lysed blood from healthy volunteers
spiked with tumor cells from three prostate cancer cell-lines (DU145,
PC3, LNCaP). For cells fixed with paraformaldehyde, cancer cell recovery
ranged from 93.6% to 97.9% with purity ranging from 97.4% to 98.4%.
There was no detectable loss of cell viability or cell proliferation
subsequent to the exposure of viable tumor cells to acoustophoresis.
For nonfixed, viable cells, tumor cell recovery ranged from 72.5%
to 93.9% with purity ranging from 79.6% to 99.7%. These data contribute
proof-in-principle that label-free microfluidic acoustophoresis can
be used to enrich both viable and fixed cancer cells from WBCs with
very high recovery and purity
Location of the breakpoint sequences of the deletion in the <i>KLK15</i> gene.
<p>The deletion in the <i>KLK15</i> gene is located between nucleotide 56 022 311 in intron 2 and nucleotide 56 025 704 in intron 1 of the <i>KLK15</i> gene, which results in a 3394-bp deletion eliminating exon 2.</p
Acoustic Enrichment of Extracellular Vesicles from Biological Fluids
Extracellular vesicles
(EVs) have emerged as a rich source of biomarkers
providing diagnostic and prognostic information in diseases such as
cancer. Large-scale investigations into the contents of EVs in clinical
cohorts are warranted, but a major obstacle is the lack of a rapid,
reproducible, efficient, and low-cost methodology to enrich EVs. Here,
we demonstrate the applicability of an automated acoustic-based technique
to enrich EVs, termed <i>acoustic trapping</i>. Using this
technology, we have successfully enriched EVs from cell culture conditioned
media and urine and blood plasma from healthy volunteers. The acoustically
trapped samples contained EVs ranging from exosomes to microvesicles
in size and contained detectable levels of intravesicular microRNAs.
Importantly, this method showed high reproducibility and yielded sufficient
quantities of vesicles for downstream analysis. The enrichment could
be obtained from a sample volume of 300 μL or less, an equivalent
to 30 min of enrichment time, depending on the sensitivity of downstream
analysis. Taken together, acoustic trapping provides a rapid, automated,
low-volume compatible, and robust method to enrich EVs from biofluids.
Thus, it may serve as a novel tool for EV enrichment from large number
of samples in a clinical setting with minimum sample preparation
Picture and illustration of the set up.
<p>(a) Photo of the acoustophoresis microfluidic system first presented by Augustsson <i>et al. </i><a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0064233#pone.0064233-Augustsson3" target="_blank">[27]</a>. (b) Cross sectional view of cell distribution in the microchannel without ultrasound (left) and with ultrasound forming an ultrasound standing wave (right). (c) Illustration of acouscoustophoresis chip. For the present study, only one of the channel segments was used allowing cells to be exposed to ultrasound.</p
Unaltered viability of BV2 microglial cell line following acoustophoretic processing (10V<sub>pp</sub> and 20V<sub>pp</sub>).
<p>BV2 cells were passed through the acoustophoresis chip with the function generator set at 0, 10 and 20 V<sub>pp</sub>. After going through the chip, the BV2 cells were seeded again for 24 and 48 h. Cell viability was measured by XTT (a), apoptotic nuclei appearance (b) and decrease of mitochondrial potential -Ψm- (c), showing no difference between experimental groups. Similar, no difference was detected by clonogenic assay (d) used to study survival and proliferation at 7 days following acoustophoretic processing. The graphs show the results from at least three separate experiments and the data are shown as means ± SD. Significance value <i>P</i><0.05, ns denotes non-significant.</p
Acoustic cell separation in a microchannel does not alter PSA secretion by prostate cancer cells.
<p>The androgen receptor (AR) expressing cell lines LNCaP and VCaP were used to evaluate the impact of acoustophoresis on PSA secretion. After acoustophoresis run at 0, 10 and 20 V<sub>pp</sub>, the secretion of PSA was measured in the absence or presence of the AR ligand R1881 (1 nM for 24 h) in the LNCaP cell line (a) and in the VCaP cell line (b). Cells not processed through the chip were used as control cells. The graphs show the results from three separate experiments and the data are shown as means ± SD. Significance value <i>P</i><0.05, ns denotes non-significant.</p
Inflammatory response of BV2 cells upon LPS challenge following acoustophoresis is not changed.
<p>After acoustophoretic processing, BV2 cells were seeded and stimulated the next day with LPS for 24 h. We observed no alteration due to acoustophoretic processing in the expression of iNOS (inducible nitric oxide synthase)(a,b), the release of proinflammatory cytokines IL-1β (χ), IL-12 (d), TNF-α (e) or the anti-inflammatory cytokine IL-10 (f). The graphs show the results from at least three separate experiments and the data are shown as means ± SD. Significance value <i>P</i><0.05, ns denotes non-significant.</p
Mitochondrial respiratory function in human leukocytes and thrombocytes are not altered following acoustophoresis.
<p>Leukocytes and thrombocytes were passed through the acoustophoresis chip run at 0, 10 and 20 V<sub>pp</sub>. Maximal respiration during using both complex I- and complex II-linked substrates for thrombocytes (a) and leukotcytes (b) was unaltered after acoustophoresis, supporting that acoustophoresis does not affect metabolic pathways important for respiration. The remaining respiratory activity following inhibition of ATP synthase with oligomycin, so called Leak or state 4 respiration, was also unaffected by acoustophoresis, in thrombocytes (c) and leukocytes (d). This data confirm no effect of acoustophoresis on inner mitochondrial membrane integrity or changed utilization of proton motive force for other purposes than ADP phosphorylation. Unprocessed cells were used as control cells. The graphs show the results from three separate experiments and the data are shown as means ± SD. Significance value <i>P</i><0.05, ns denotes non-significant.</p
Association of the SNP rs10993994 with the Gleason score of the biopsy and prostatectomy specimens and pT-stage.
<p>Association of the SNP rs10993994 with the Gleason score of the biopsy and prostatectomy specimens and pT-stage.</p
Correlation of <i>MSMB</i> and <i>MSMB-NCOA4</i> expression according to qRT-PCR.
<p><i>MSMB</i> expression was positively correlated with expression of <i>MSMB-NCOA4</i>.</p