27 research outputs found
Molecular Characterization and Expression Profile Analysis of Heat Shock Transcription Factors in Mungbean
Heat shock transcription factors (Hsfs) are essential elements in plant signal transduction pathways that mediate gene expression in response to various abiotic stresses. Mungbean (Vigna radiata) is an important crop worldwide. The emergence of a genome database now allows for functional analysis of mungbean genes. In this study, we dissect the mungbean Hsfs using genome-wide identification and expression profiles. We characterized a total of 24 VrHsf genes and classified them into three groups (A, B, and C) based on their phylogeny and conserved domain structures. All VrHsf genes exhibit highly conserved exon-intron organization, with two exons and one intron. In addition, all VrHsf proteins contain 16 distinct motifs. Chromosome location analysis revealed that VrHsf genes are located on 8 of the 11 mungbean chromosomes, and that seven duplicated gene pairs had formed among them. Moreover, transcription patterns of VrHsf genes varied in different tissues, indicating their different roles in plant growth and development. We identified multiple stress related cis-elements in VrHsf promoter regions 2 kb upstream of the translation initiation codons, and the expression of most VrHsf genes was altered under different stress conditions, suggesting their potential functions in stress resistance pathways. These molecular characterization and expression profile analyses of VrHsf genes provide essential information for further function investigation
Transverse structural modulation in nematic SrAl and elucidation of its origin in the BaAl family of compounds
At ambient conditions SrAl adopts the BaAl structure type with space
group . It undergoes a charge-density-wave (CDW) transition at
= 243 K, followed by a structural transition at = 87 K.
Temperature-dependent single-crystal X-ray diffraction (SXRD) leads to the
observation of incommensurate superlattice reflections at with at 200 K. The CDW has
orthorhombic symmetry with the superspace group , where
is a subgroup of of index 2. Atomic displacements represent a
transverse wave, and they are mainly along one of the diagonal directions of
the -centered unit cell. The breaking of fourfold rotational symmetry is
indicative of the presence of nematic order in the material. The orthorhombic
phase realized in SrAl is analogous to that found in EuAl, albeit with
the presence of higher order satellite reflections (up to ) and a
shorter modulation wave vector. A possible non-trivial band topology has
prevented the determination by density functional theory (DFT) of the mechanism
of CDW formation. However, DFT reveals that Al atoms dominate the density of
states near the Fermi level, thus, corroborating the SXRD measurements.
SrAl remains incommensurately modulated at the structural transition, where
the symmetry lowers from orthorhombic to -unique monoclinic. We
have identified a simple criterion, that correlates the presence of a phase
transition with the interatomic distances. Only those compounds
AlGa ( = Ba, Eu, Sr, Ca; ) undergo phase
transitions, for which the ratio falls within the narrow range
The large area detector onboard the eXTP mission
The Large Area Detector (LAD) is the high-throughput, spectral-timing instrument onboard the eXTP mission, a flagship
mission of the Chinese Academy of Sciences and the China National Space Administration, with a large European
participation coordinated by Italy and Spain. The eXTP mission is currently performing its phase B study, with a target
launch at the end-2027. The eXTP scientific payload includes four instruments (SFA, PFA, LAD and WFM) offering
unprecedented simultaneous wide-band X-ray timing and polarimetry sensitivity. The LAD instrument is based on the
design originally proposed for the LOFT mission. It envisages a deployed 3.2 m2 effective area in the 2-30 keV energy
range, achieved through the technology of the large-area Silicon Drift Detectors - offering a spectral resolution of up to
200 eV FWHM at 6 keV - and of capillary plate collimators - limiting the field of view to about 1 degree. In this paper
we will provide an overview of the LAD instrument design, its current status of development and anticipated
performance
SemSUM: Semantic Dependency Guided Neural Abstractive Summarization
In neural abstractive summarization, the generated summaries often face semantic irrelevance and content deviation from the input sentences. In this work, we incorporate semantic dependency graphs about predicate-argument structure of input sentences into neural abstractive summarization for the problem. We propose a novel semantics dependency guided summarization model (SemSUM), which can leverage the information of original input texts and the corresponding semantic dependency graphs in a complementary way to guide summarization process. We evaluate our model on the English Gigaword, DUC 2004 and MSR abstractive sentence summarization datasets. Experiments show that the proposed model improves semantic relevance and reduces content deviation, and also brings significant improvements on automatic evaluation ROUGE metrics
B-box Proteins in Arachis duranensis: Genome-Wide Characterization and Expression Profiles Analysis
B-box (BBX) proteins are important factors involved in plant growth and developmental regulation, and they have been identified in many species. However, information on the characteristics and transcription patterns of BBX genes in wild peanut are limited. In this study, we identified and characterized 24 BBX genes from a wild peanut, Arachis duranensis. Many characteristics were analyzed, including chromosomal locations, phylogenetic relationships, and gene structures. Arachis duranensis B-box (AdBBX) proteins were grouped into five classes based on the diversity of their conserved domains: I (3 genes), II (4 genes), III (4 genes), IV (9 genes), and V (4 genes). Fifteen distinct motifs were found in the 24 AdBBX proteins. Duplication analysis revealed the presence of two interchromosomal duplicated gene pairs, from group II and IV. In addition, 95 kinds of cis-acting elements were found in the genes’ promoter regions, 53 of which received putative functional predictions. The numbers and types of cis-acting elements varied among different AdBBX promoters, and, as a result, AdBBX genes exhibited distinct expression patterns in different tissues. Transcriptional profiling combined with synteny analysis suggests that AdBBX8 may be a key factor involved in flowering time regulation. Our study will provide essential information for further functional investigation of AdBBX genes
An Optical Biosensor for Monitoring Antigen Recognition Based on Surface Plasmon Resonance Using Avidin-Biotin System
A novel optical biosensor based on simultaneous multiwave length detection surface plasmon resonance (SPR) has been developed for immunosensing. The sensor is designed on the basis of fixing angle of incidence and measuring the reflected intensities of light in the wavelength range of 400–800 nm. The SPR spectra are shown in terms of reflected light intensity verus wavelength of incident light. The intensity of the reflected light is the minimum at the resonant wavelength. The biorecognition surface, formed on a chemically modified gold layer, consists of avidin that is specifically bound with biotin. These sensing membranes were self-assembled on gold layer. The processes of sensing monolayer formation were studied in real time through observing the change of resonant wavelength. The modified surface was used as a model immunosensor and to detect successfully the human factor B (Bf). The Bf was determined in the concentration range of 0.5~100 μg/mL. Under optimum experimental conditions, the sensor has a good repeatability, reversibility and selectivity
Characterization and Comparative Analysis of RWP-RK Proteins from Arachis duranensis, Arachis ipaensis, and Arachis hypogaea
RWP-RK proteins are important factors involved in nitrate response and gametophyte development in plants, and the functions of RWP-RK proteins have been analyzed in many species. However, the characterization of peanut RWP-RK proteins is limited. In this study, we identified 16, 19, and 32 RWP-RK members from Arachis duranensis, Arachis ipaensis, and Arachis hypogaea, respectively, and investigated their evolution relationships. The RWP-RK proteins were classified into two groups, RWP-RK domain proteins and NODULE-INCEPTION-like proteins. Chromosomal distributions, gene structures, and conserved motifs of RWP-RK genes were compared among wild and cultivated peanuts. In addition, we identified 12 orthologous gene pairs from the two wild peanut species, 13 from A. duranensis and A. hypogaea, and 13 from A. ipaensis and A. hypogaea. One, one, and seventeen duplicated gene pairs were identified within the A. duranensis, A. ipaensis, and A. hypogaea genomes, respectively. Moreover, different numbers of cis-acting elements in the RWP-RK promoters were found in wild and cultivated species (87 in A. duranensis, 89 in A. ipaensis, and 92 in A. hypogaea), and as a result, many RWP-RK genes showed distinct expression patterns in different tissues. Our study will provide useful information for further functional and evolutionary analysis of the RWP-RK genes