Figure 9

<p>Correlation of serum triglyceride measure with different triacylglycerol species as a function of (A) the amount, and (B) the number of fatty acid carbons.</p

Figure 4

<p>Twin-normalized Spearman rank correlations of (A) two most abundant lysophosphatidylcholine and (B) ether phospholipid species with clinical variables. ^*<i>p</i><0.05, ^**<i>p</i><0.01.</p

Figure 8

<p>Correlations with classical lipid parameters using Spearman rank correlations across all individuals (<i>N</i> = 48). Only lipids with <i>p</i><0.1 are reported for triglyceride and total cholesterol correlations. ^*<i>p</i><0.05, ^**<i>p</i><0.01, ***<i>p</i><0.001.</p

Figure 2

<p>Correlation maps for clinical variables. (A) Each individual considered separately (N = 48). (B) Twin pair normalized (N = 24).</p

Figure 3

<p>Partial least squares discriminant analysis (PLS/DA) of lipidomics profiles for obesity discordant co-twins, utilizing only the 133 identified peaks and two classes (obese and non-obese co-twins) to build the model. Three latent variables were used (Q² = 47%). (A) PLS/DA score plot. Genders and twin-pair identifiers are marked for each sample, although this information was not used to build the model. (B) Fold changes for most important variables based on VIP analysis contributing to the PLS/DA model.</p

Figure 6

<p>Twin-normalized Spearman rank correlations of lipids with different fat depots: (A) BMI, (B) total body fat, (C) subcutaneous fat, and (D) intra-abdominal fat. ^*<i>p</i><0.05, ^**<i>p</i><0.01.</p

Physical and biochemical characteristics of the 24 monozygotic twin pairs.

<p>Data are median (interquartile range). ^*obese vs. non-obese co-twins, paired Wilcoxon's test. ^a n = 19 pairs. ^b n = 21 pairs.</p

Figure 5

<p>Correlation plots for selected sphingomyelin species and clinical variables in (A) individual twins, and (B) twin pairs.</p

Time course of ORP11, ORP3, and ORP8 mRNA changes upon SGBS cell adipogenic differentiation.

<p>A. Phase contrast images of SGBS preadipocytes (0 D) and differentiating adipocytes on days 10, 14, and 22. Bars, 200 µm. B. The ORP mRNA levels were quantified at the differentiation time points indicated, as fold changes relative to preadipocytes (day 0). The data represents mean ± S.E., n = 3.</p

Figure 7

<p>Twin-normalized Spearman rank correlations of lipids with (A) M-value and (B) insulin. ^*<i>p</i><0.05, ^**<i>p</i><0.01.</p