Lack of IL-10 increases cellular influx into the lungs and delays recovery during RSV infection.

<p>BALB/c mice and IL-10^−/− mice were infected with 10⁶ PFU RSV i.n. (day 0). (A) Illness was monitored daily by changes in weight for 14 days after RSV infection; percentage of original weight (day 0) is shown. (B) Copies of the RSV L gene were quantified in the lung on day 4 post infection using qPCR. (C) Total numbers of cells in the lung and BAL were enumerated on day 4 and 8 from naïve or RSV infected mice. (D) Total numbers of neutrophils in the BAL were quantified using differential cell counting of H&E stained cytospins slides on day 4 and 8 post infection. (E) Total numbers of NK cells (CD3⁻ NKp46⁺) and CD3 gated CD4⁺Foxp3⁻ and CD8⁺ T cells in the lung were quantified using flow cytometry on day 4 and 8 post RSV infection. Error bars indicate the SEM. The data is representative of three independent experiments with n = 4–5 mice per group.</p

Lack of IL-10 increases chemokine and cytokine production in the airways during RSV infection.

<p>BAL samples from BALB/c and IL-10^−/− mice were analyzed for indicated cytokines and chemokines on day 4 and 8 after RSV infection using Luminex. A group of naive BALB/c controls are included in the day 4 data. Error bars indicate the SEM. The data are pooled from two independent experiments with n = 4–5 mice per group.</p

Additional file 1: of What gives rise to clinician gut feeling, its influence on management decisions and its prognostic value for children with RTI in primary care: a prospective cohort study

Web Appendix Table S1. Determinants: univariable associations between sociodemographic, parent-reported symptoms, clinician-reported observations, clinician profile and gut feeling. This table presents the univariable associations between child and clinician variables and gut feeling. (DOCX 21 kb

Increased frequency of IFN-γ-expressing T cells in IL-10^−/− mice after RSV infection.

<p>BALB/c mice were infected with 10⁶ PFU RSV i.n. (day 0). CD3⁺ gated, CD4⁺ and CD8⁺ T cells from lungs on day 8 post RSV infection were analyzed by flow cytometry. Representative plots of intracellular IFN-γ expression after a 3 hr PMA/ionomycin restimulation are shown. In addition, quantifications of the frequencies of IFN-γ expression in CD4⁺ T cells and CD8⁺ T cells are shown. The data are representative of three independent experiments with n = 4–5 mice per group.</p

Blocking IL-10R signalling increases cellular influx into the lungs and delays recovery during RSV infection.

<p>BALB/c mice were infected with 10⁶ PFU RSV i.n. (day 0). Where indicated, mice were injected with anti-IL-10R antibody on day -1 (i.p.), 3 (i.p. and i.n) and 6 (i.p.) post RSV infection. Control groups were injected with rat IgG. (A) Illness was monitored daily by changes in weight for 8 days after RSV infection; the percentage of original weight is shown. (B) Viral titer was measured in the lung on day 4 post infection by quantifying RSV L gene copies by qPCR. (C) Total numbers of cells in the lung and BAL were enumerated on day 4 and 8 from naïve or RSV infected mice. (D) Total numbers of neutrophils in the BAL were quantified using differential cell counting of H&E stained cytospins slides on day 4 and 8 post infection. (E) Total numbers of NK cells (CD3⁻ NKp46⁺) and CD3-gated, CD4⁺Foxp3⁻ and CD8⁺ T cells in the lung were quantified using flow cytometry on day 4 and 8 post RSV infection. Error bars indicate the SEM. The data are representative of two independent experiments with n = 4–5 mice per group.</p