Social and Pragmatic Language in Autistic Children

Two research studies, one pinpointing difference in areas of the brain used in pragmatic language activities between neurotypical and autistic children, and the other examining pragmatic language development in children with autism over time are summarized and compared

The Impact of Homelessness on Childhood Stress and Resilience

Through volunteer experiences at Restoration Urban Ministry’s Homework Hangout Program, this student interacted with homeless children. That experience, paired with a review of research on the effects of homelessness on children, leads her to conclude that, although stress can have serious negative consequences in the biological, cognitive, and psychosocial realms of development, some children exhibit resiliency and are able to reduce the effects of stress. Suggests that stress produced by homelessness can be minimized through prevention and intervention such as fostering healthy parent-child relationships, providing practical services and health screenings can all create and nurture resilience in children

Guaging Student Levels of Comfort and Approachability

As part of Anthropology 103’s Ethnographies of Parkland College Project, this is a summary of the qualitative study conducted by the author and two other students which examined the approachability of strangers, especially in regards to how comfortable someone feels when been approached by someone of their own demographics versus very different demographics (age, gender, and ethnicity). The group hypothesized that Parkland students and faculty would either display a lot of openness to diversity, or they would be close-minded. However, data proved to demonstrate a mixture of results, as well as more complicated themes. They concluded that students and faculty members at Parkland feel that they are open to diversity, but noticed that most people were either cautious of everyone in general or had slight preferences toward people of certain demographics, usually their own

Mechanotransduction of mitochondrial AMPK and its distinct role in flow-induced breast cancer cell migration

The biophysical microenvironment of the tumor site has significant impact on breast cancer progression and metastasis. The importance of altered mechanotransduction in cancerous tissue has been documented, yet its role in the regulation of cellular metabolism and the potential link between cellular energy and cell migration remain poorly understood. In this study, we investigated the role of mechanotransduction in AMP-activated protein kinase (AMPK) activation in breast cancer cells in response to interstitial fluid flow (IFF). Additionally, we explored the involvement of AMPK in breast cancer cell migration. IFF was applied to the 3D cell-matrix construct. The subcellular signaling activity of Src, FAK, and AMPK was visualized in real-time using fluorescent resonance energy transfer (FRET). We observed that breast cancer cells (MDA-MB-231) are more sensitive to IFF than normal epithelial cells (MCF-10A). AMPK was activated at the mitochondria of MDA-MB-231 cells by IFF, but not in other subcellular compartments (i.e., cytosol, plasma membrane, and nucleus). The inhibition of FAK or Src abolished flow-induced AMPK activation in the mitochondria of MDA-MB-231 cells. We also observed that global AMPK activation reduced MDA-MB-231 cell migration. Interestingly, specific AMPK inhibition in the mitochondria reduced cell migration and blocked flow-induced cell migration. Our results suggest the linkage of FAK/Src and mitochondria-specific AMPK in mechanotransduction and the differential role of AMPK in breast cancer cell migration depending on its subcellular compartment-specific activation