Obstacles and Opportunities of Using a Mobile App for Marine Mammal Research

This study investigates the use of a mobile application, Whale mAPP, as a citizen science tool for collecting marine mammal sighting data. In just over three months, 1261 marine mammal sightings were observed and recorded by 39 citizen scientists in Southeast Alaska. The resulting data, along with a preliminary and post-Whale mAPP questionnaires, were used to evaluate the tool’s scientific, educational, and engagement feasibility. A comparison of Whale mAPP Steller sea lion distribution data to a scientific dataset were comparable (91% overlap) given a high enough sample size (n = 73) and dense spatial coverage. In addition, after using Whale mAPP for two weeks, citizen scientists improved their marine mammal identification skills and self-initiated further learning, representing preliminary steps in developing an engaging citizen science project. While the app experienced high initial enthusiasm, maintaining prolonged commitment represents one of the fundamental challenges for this project. Increasing participation with targeted recruitment and sustained communication will help combat the limitations of sample size and spatial coverage. Overall, this study emphasizes the importance of early evaluation of the educational and scientific outcomes of a citizen science project, so that limitations are recognized and reduced

Obstacles and Opportunities of Using a Mobile App for Marine Mammal Research

This study investigates the use of a mobile application, Whale mAPP, as a citizen science tool for collecting marine mammal sighting data. In just over three months, 1261 marine mammal sightings were observed and recorded by 39 citizen scientists in Southeast Alaska. The resulting data, along with a preliminary and post-Whale mAPP questionnaires, were used to evaluate the tool’s scientific, educational, and engagement feasibility. A comparison of Whale mAPP Steller sea lion distribution data to a scientific dataset were comparable (91% overlap) given a high enough sample size (n = 73) and dense spatial coverage. In addition, after using Whale mAPP for two weeks, citizen scientists improved their marine mammal identification skills and self-initiated further learning, representing preliminary steps in developing an engaging citizen science project. While the app experienced high initial enthusiasm, maintaining prolonged commitment represents one of the fundamental challenges for this project. Increasing participation with targeted recruitment and sustained communication will help combat the limitations of sample size and spatial coverage. Overall, this study emphasizes the importance of early evaluation of the educational and scientific outcomes of a citizen science project, so that limitations are recognized and reduced

Leveraging structure determination with fragment screening for infectious disease drug targets: MECP synthase from Burkholderia pseudomallei

As part of the Seattle Structural Genomics Center for Infectious Disease, we seek to enhance structural genomics with ligand-bound structure data which can serve as a blueprint for structure-based drug design. We have adapted fragment-based screening methods to our structural genomics pipeline to generate multiple ligand-bound structures of high priority drug targets from pathogenic organisms. In this study, we report fragment screening methods and structure determination results for 2C-methyl-D-erythritol-2,4-cyclo-diphosphate (MECP) synthase from Burkholderia pseudomallei, the gram-negative bacterium which causes melioidosis. Screening by nuclear magnetic resonance spectroscopy as well as crystal soaking followed by X-ray diffraction led to the identification of several small molecules which bind this enzyme in a critical metabolic pathway. A series of complex structures obtained with screening hits reveal distinct binding pockets and a range of small molecules which form complexes with the target. Additional soaks with these compounds further demonstrate a subset of fragments to only bind the protein when present in specific combinations. This ensemble of fragment-bound complexes illuminates several characteristics of MECP synthase, including a previously unknown binding surface external to the catalytic active site. These ligand-bound structures now serve to guide medicinal chemists and structural biologists in rational design of novel inhibitors for this enzyme

Obstacles and Opportunities of Using a Mobile App for Marine Mammal Research

This study investigates the use of a mobile application, Whale mAPP, as a citizen science tool for collecting marine mammal sighting data. In just over three months, 1261 marine mammal sightings were observed and recorded by 39 citizen scientists in Southeast Alaska. The resulting data, along with a preliminary and post-Whale mAPP questionnaires, were used to evaluate the tool’s scientific, educational, and engagement feasibility. A comparison of Whale mAPP Steller sea lion distribution data to a scientific dataset were comparable (91% overlap) given a high enough sample size (n = 73) and dense spatial coverage. In addition, after using Whale mAPP for two weeks, citizen scientists improved their marine mammal identification skills and self-initiated further learning, representing preliminary steps in developing an engaging citizen science project. While the app experienced high initial enthusiasm, maintaining prolonged commitment represents one of the fundamental challenges for this project. Increasing participation with targeted recruitment and sustained communication will help combat the limitations of sample size and spatial coverage. Overall, this study emphasizes the importance of early evaluation of the educational and scientific outcomes of a citizen science project, so that limitations are recognized and reduced