Diurnal expression of <i>F. vesca PIP</i> aquaporins.

<p>Diurnal expression pattern of <i>FvPIP</i> genes in leaves of <i>F. vesca</i> on two consecutive days. Grey columns – control plants, black columns – drought-stressed plants. Data are means+SE, <i>n</i> = 3 biological replicates. Different letters annotate statistically significant differences determined by LSD following repeated measurements ANOVA (<i>P</i><0.05). The difference between treatments was significant for (a), (b), (c), and (d).</p

<i>F. vesca</i> PIP isoform-specific and reference gene primers.

<p><i>F. vesca</i> PIP isoform-specific and reference gene primers.</p

Deduced protein sequences of <i>F. vesca</i> PIP aquaporins.

<p>An alignment of <i>F. vesca</i> PIP deduced protein sequences. Blue – transmembrane domains (TM); red – NPA motif; green highlight – residues of the aromatic/arginine selectivity filter involved in determining water specificity. Asterisk denotes conserved sites. N stands for amino-terminal region and C stands for carboxy-terminal region of the protein.</p

Relative <i>PIP</i> expression in leaves and roots of <i>F. vesca</i> under different levels of drought stress.

<p>Expression of four <i>F. vesca PIP</i> aquaporin genes after six days of drought stress (a, c, e, g) and upon re-watering (b, d, f, h). C – control plants; D25– plants receiving 25% of the control irrigation; D0 − plants with no irrigation. Data are means+SE, <i>n</i> = 4 plants. Different letters denote statistically significant differences within each time point determined by LSD following one way ANOVA (<i>P</i><0.05).</p

Correlation between substrate moisture content and relative expression of <i>FvPIP</i> genes.

<p><i>FvPIP2;1</i> (a, b), <i>FvPIP2;2</i> (c, d), <i>FvPIP1;1</i> (e,f), <i>FvPIP1;2</i> (g, h) in leaves (a, c, e, g) and roots (b, d, f, h). Regression lines, correlation coefficients and probabilities are given for statistically significant relationships.</p