Radioautographic Evidence of 3H-Tryptophan Incorporation in Secretory Cells of Rat Submandibular Glands

Tritiated tryptophan was injected intravenously into male rats, and the submandibular glands were removed at time intervals up to three hours after injection. Grain counts were made on light and electron microscope radioautographs to determine the effects on the amino acid.Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/67201/2/10.1177_00220345710500064601.pd

Biochemical and Morphological Studies of Rat Submandibular Gland: III. Effects of Testosterone Treatment on Proteins of Granule-Rich Fraction

Mature adult male rats and castrated and testosterone-treated castrated adult rats were injected with pilocarpine HGL and 3-H-lysine and sacrificed sequentially over an eight-hour time period. Following homogenization and differential centrifugation, three subcellular fractions from each group of animals were analyzed by gel electrophoresis and liquid scintillation. Two proteins in the granule-rich fraction appeared in larger amounts on the densitometric scans and appeared to represent a larger proportion of newly synthesized proteins than the other proteins.Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/66712/2/10.1177_00220345770560030901.pd

Protein synthesis in vitro , in the presence of Ca(OH) 2 -containing pulp-capping medicaments

Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/73494/1/j.1600-0714.1983.tb00348.x.pd

The Expression of the Epithelial Blood-group Substances: Normal and Malignant Tissues

The blood-group isoantigens are macromolecules localized to the plasma membranes of certain epithelial tissues.2,11-15 These substances are not detectable on the epithelium once it has undergone malignant transformation.2,9,13 Results of this investigation have demonstrated that the loss of detectability of the blood-group isoantigens does not appear to be related to a "masking" effect by an increase in surface sialic acid. Using fluorescein-labeled lectins specific for sugar subunits which are components of the blood-group oligosaccharide chain, it was found that the malignant cells and cells of the para basal layer of normal oral epithelium had high levels of N-acetyl-D-glucosamine (GlcNAC), the subterminal sugar residue of the blood-group chain. The basal cells of normal epithelium and a minority of the malignant cells demonstrated levels of D-galactose-N-acetyl-D-galactosamine, which are the most proximal blood-group sugar subunits, as well as subunits of other membrane antigens. Our results indicate that malignant cells seem to be capable of synthesizing the blood-group oligosaccharide chains to the same level as the normal cells of the para basal layer of stratified squamous epithelium. This level is just subjacent to the terminal D-galactose residue of the blood-group precursor chain. Increased or decreased differentiation characteristics of squamous cell carcinomas did not alter the level of blood-group synthesis. However, there may be a correlation between the level of synthesis of these antigens and the ability of the cells to demonstrate motility and to proliferate.Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/66719/2/10.1177_00220345800590112001.pd

Biochemical and Morphological Studies of Rat Submandibular Gland: II. Partial Purification of Proteins from Granule-Rich Fraction

Soluble proteins derived from a centrifuged and filtered granule-rich fraction of homogenized rat submandibular gland were analyzed by gel filtration, ion-exchange chromatography, and polyacrylamide gel electrophoresis. Both the granule-rich fraction and final supernatant fraction contained alkaline esterase activity. The major protein component, derived from granules of the convoluted tubules, was further resolved into a series of peptides ranging in molecular weight from 9,000 to 55,000 daltons.Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/67196/2/10.1177_00220345750540053301.pd

Blood group substances as differentiation markers in human dento-gingival epithelium

Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/65594/1/j.1600-0765.1987.tb02054.x.pd

The inhibitory effects of a dermal extract upon granulation tissue

Injection of a water-soluble dermal extract into adult rats resulted in depression of DNA synthesis in granulation tissue at 3 different sites of injury, but not in epithelium. This suggests that connective tissue proliferation may be controlled in part by a chalone-like mechanism.Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/42665/1/18_2005_Article_BF01937006.pd

Search for cyclotron resonance in cells in vitro

There are a number of reports of the plasma membrane transport of Ca 2+ in biological systems being enhanced by low frequency electromagnetic fields (EMF), including reports that the enhancement involves a resonance-type response at the cyclotron frequency for Ca 2+ ions for geomagnetic values of the magnetic field. Using the fluorescent probe fura2, we find no evidence for changes in cytosolic calcium concentration in BALB/c3T3, L929, V-79, and ROS, a rat osteosarcoma cell line, at the application of both resonant and nonresonant EMF.Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/38292/1/2250100203_ftp.pd

Cytotoxicity and Dentin Permeability of Carbamide Peroxide and Hydrogen Peroxide Vital Bleaching Materials, in vitro

There has been recent concern about the inadvertent exposure of dentin with patent tubules as well as gingiva to bleaching systems containing 10-15% carbamide peroxide or 2-10% hydrogen peroxide for more than a few minutes. The aims of the present study were: (1) to determine the cytotoxicity of dilutions of hydrogen peroxide in cell culture; (2) to measure hydrogen peroxide diffusion from bleaching agents through dentin in vitro; and (3) to determine the risk of hydrogen peroxide-induced cytotoxicity from exposure of dentin to these vital bleaching agents. The 50% inhibitory dose (ID50) of hydrogen peroxide to succinyl dehydrogenase activity in cultured cells was found to be 0.58 mmol/L after 1 h. All bleaching materials demonstrated diffusion of hydrogen peroxide through dentin in an "in vitro pulp chamber" device. The one- and six-hour diffusates of all bleaching agents through 0.5-mm dentin exceeded the ID50 in monolayer cultures. Inhibition of succinyl dehydrogenase activity corresponded to the amount of hydrogen peroxide that can rapidly diffuse through dentin in vitro and reach concentrations which are toxic to cultured cells in less than 1 h.Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/66707/2/10.1177_00220345930720051501.pd

Cytotoxicity of dental composites and other materials in a new in vitro device

Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/73901/1/j.1600-0714.1988.tb01304.x.pd