Student Knowledge, Attitudes, and Use of Evidence-Based Concepts Following an Educational Intervention

Context: While evidence-based practice (EBP) concepts are being taught in health profession education programs, models of instruction and effectiveness of these models are not evident in athletic training. Objective: To evaluate the effectiveness of the Evidence-Based Teaching Model (EBTM) in increasing student knowledge, attitudes, and use of evidence-based concepts. Design: Within subjects design with pre- and post-test evaluations of students\u27 knowledge, attitudes, and intended use using the researcher-developed Evidence-Based Concepts: Knowledge, Attitudes and Use (EBCKAU) survey. Setting: CAATE-accredited undergraduate programs. Participants: Eighty-two students from a stratified purposeful sample of 9 institutions were enrolled in the study, 78 students (95%) completed the knowledge portion of the survey, while 68 students (83%) fully completed the knowledge, attitudes, and use portions of the survey. Data Collection and Analysis: The EBCKAU survey was used to assess student factors relating to EBP through multiple choice, Likert scale, and open-ended questions. Results: Students significantly increased their knowledge, confidence in knowledge, familiarity with, and confidence in use of EBP skills following the EBTM. Prior to the EBTM, students earned a mean knowledge score of 50%. This improved to 66% post-EBTM. Students\u27 interest and perceived importance scores did not increase. Barriers to student use of EBP included time, available resources, ACI open-mindedness, and experience. Conclusions: The EBTM was effective in improving student factors related to knowledge and use of EBP concepts. To our knowledge, this is the first published teaching model that assessed student outcomes related to EBP in athletic training education

Educator Perceptions of the Evidence-Based Teaching Model in Undergraduate Athletic Training Education

Context: While research recommends that health professions expand the instruction and use of evidence-based practice (EBP) due to the individualized approach to patient health care, few examples of the incorporation of EBP into academic courses exist in athletic training. Objective: To evaluate educators\u27 perceptions of the Evidence-Based Teaching Model (EBTM) as a strategy to introduce EBP concepts to athletic training (AT) students. Design: Qualitative program evaluation including semi-structured interviews. Setting: Institutions that sponsor CAATE-accredited professional undergraduate programs. Participants: Stratified purposeful sampling of 9 experienced educators (2 males, 7 females average years teaching 8 plus or minus 5 years) teaching therapeutic modalities or rehabilitation were trained in the EBTM and interviewed regarding their experience. Measures: Educators\u27 experiences regarding implementation of the EBTM. Coded categories were triangulated via member checks and peer review to establish trustworthiness of the findings. Results: Educators valued the EBTM as a method to implement evidence-based concepts within a short time frame in their course, and perceived it as a user-friendly and effective teaching tool. Assignments requiring direct interaction between students and clinical instructors were considered most favorable. Training materials provided educators with a new perspective of how to implement EBP at the professional level. Conclusions: Implementation of the EBTM helped educators attain their goals of expanding evidence-based concepts within professional undergraduate curricula and increasing student and clinical instructor interaction. Overall, the EBTM provided a mechanism to begin incorporation of EBP concepts in athletic training curricula

Sheathless capillary electrophoresis-mass spectrometry for anionic metabolic profiling

The performance of CE coupled on-line to MS via a sheathless porous tip sprayer was evaluated for anionic metabolic profiling. A representative metabolite mixture and biological samples were used for the evaluation of various analytical parameters, such as peak efficiency (plate numbers), migration time and peak area repeatability, and LODs. The BGE, i.e. 10% acetic acid (pH 2.2), previously used for cationic metabolic profiling was now assessed for anionic metabolic profiling by using MS detection in negative ion mode. For test compounds, RSDs for migration times and peak areas were below 2 and 11%, respectively, and plate numbers ranged from 60 000 to 40 0000 demonstrating a high separation efficiency. Critical metabolites with low or no retention on reversed-phase LC could be efficiently separated and selectively analyzed by the sheathless CE-MS method. An injection volume of only circa 20 nL resulted in LODs between 10 and 200 nM (corresponding to an amount of 0.4-4 fmol), which was an at least tenfold improvement as compared to LODs obtained by conventional CE-MS approaches for these analytes. The methodology was applied to anionic metabolic profiling of glioblastoma cell line extracts. Overall, a sheathless CE-MS method has been developed for highly efficient and sensitive anionic metabolic profiling studies, which can also be used for cationic metabolic profiling studies by only switching the MS detection and separation voltage polarity.Analytical BioScience

Graft remodeling during growth following anterior cruciate ligament reconstruction in skeletally immature sheep

INTRODUCTION: Ruptures of the anterior cruciate ligament are being diagnosed with increasing frequency in skeletally immature individuals. It was our aim to investigate the graft remodelling process following an autologous, transphyseal reconstruction of the anterior cruciate ligament (ACL) in skeletally immature sheep. We hypothesized that the ligamentisation process in immature sheep is quicker and more complete when compared to adult sheep. MATERIALS AND METHODS: Skeletally immature sheep with an age of 4 months underwent a fully transphyseal ACL reconstruction using an autologous tendon. The animals were subsequently sacrificed at 3, 6, 12 and 24 weeks following surgery. Each group was characterised histomorphometrically, by immunostaining (VEGF, SMA), by transmission electron microscopy (TEM) and biomechanically (UFS Roboter). RESULTS: The histomorphometric analysis and presence of VEGF and SMA positive cells demonstrated a rapid return to a ligament like structure. The biomechanical analysis revealed an anteroposterior translation that was still increased even 6 months following surgery. CONCLUSION: As in adult sheep models, the remodeling of a soft tissue graft used for ACL reconstruction results in a biomechanically inferior substitute. However, the immature tissue seems to remodel faster and more complete when compared to adults

Bone marrow mesenchymal stem cells do not enhance intra-synovial tendon healing despite engraftment and homing to niches within the synovium

Intra-synovial tendon injuries display poor healing, which often results in reduced functionality and pain. A lack of effective therapeutic options has led to experimental approaches to augment natural tendon repair with autologous mesenchymal stem cells (MSCs) although the effects of the intra-synovial environment on the distribution, engraftment and functionality of implanted MSCs is not known. This study utilised a novel sheep model which, although in an anatomically different location, more accurately mimics the mechanical and synovial environment of the human rotator cuff, to determine the effects of intra-synovial implantation of MSCs

Single-cell metabolomics by mass spectrometry: advances, challenges, and future applications

Cellular heterogeneity is a phenomenon that is often observed but poorly understood. Single-cell metabolomics can provide insights into the phenotypical variations between individual cells. Recent advances in microfluidics, micromanipulation, image analysis, and automation allowed for high-throughput isolation of single cells in a minimally disruptive manner as to not affect the cell metabolism. Coupled with new innovations in mass spectrometry-based analytical techniques, single cell metabolomics stands at the cusp of becoming an established field. In this review, some of the recent single cell isolation platforms that are especially suited for metabolomics will be highlighted, as well as the recent trends in mass spectrometry-based single cell platforms. Additionally, some of the limitations of single-cell metabolomics and its recent applications will be briefly discussed. (c) 2019 Elsevier B.V. All rights reserved.Analytical BioScience