A community resource for high-throughput quantitative RT-PCR analysis of transcription factor gene expression in -0

T ampification. The derivative of fluorescence intensity is shown on the y-axis. Separation of PCR products on 3% (v/w) agarose gels following electrophoresis (C) confirmed the presence of unique amplicons of the expected size for most reactions. Few reactions yielded no products (indicated by arrow).<p><b>Copyright information:</b></p><p>Taken from "A community resource for high-throughput quantitative RT-PCR analysis of transcription factor gene expression in "</p><p>http://www.plantmethods.com/content/4/1/18</p><p>Plant Methods 2008;4():18-18.</p><p>Published online 8 Jul 2008</p><p>PMCID:PMC2490690.</p><p></p

A community resource for high-throughput quantitative RT-PCR analysis of transcription factor gene expression in -2

Resis on 3% (v/w) agarose gels (A) and by dissociation curves with a single peak (B to D). Typical real-time RT-PCR amplification plots of three reference gene transcripts (E to G).<p><b>Copyright information:</b></p><p>Taken from "A community resource for high-throughput quantitative RT-PCR analysis of transcription factor gene expression in "</p><p>http://www.plantmethods.com/content/4/1/18</p><p>Plant Methods 2008;4():18-18.</p><p>Published online 8 Jul 2008</p><p>PMCID:PMC2490690.</p><p></p

A community resource for high-throughput quantitative RT-PCR analysis of transcription factor gene expression in -1

045 TF primer pairs (right).<p><b>Copyright information:</b></p><p>Taken from "A community resource for high-throughput quantitative RT-PCR analysis of transcription factor gene expression in "</p><p>http://www.plantmethods.com/content/4/1/18</p><p>Plant Methods 2008;4():18-18.</p><p>Published online 8 Jul 2008</p><p>PMCID:PMC2490690.</p><p></p

A community resource for high-throughput quantitative RT-PCR analysis of transcription factor gene expression in -3

Nt organs with three replicate measurements of each cDNA preparation. A low value for the average expression stability M, as calculated by geNORM software, indicates more stable expression throughout the various organs.<p><b>Copyright information:</b></p><p>Taken from "A community resource for high-throughput quantitative RT-PCR analysis of transcription factor gene expression in "</p><p>http://www.plantmethods.com/content/4/1/18</p><p>Plant Methods 2008;4():18-18.</p><p>Published online 8 Jul 2008</p><p>PMCID:PMC2490690.</p><p></p