Effects of cooking methods and in-vitro digestion on the digestibility and antioxidant properties of ngari (a fermented fish product of India)

879-888Ngari is a popular ethnic fermented fish product from North-Eastern India. It is consumed after heat process either as a part of regular meal or as a condiment. However, there is no adequate knowledge on the digestion of ngari and the bioactive potential of the digest. The present study was aimed to evaluate the effect of different heat processes viz., air frying, roasting and sautéing on the chemical attributes and in-vitro digestion characteristics of ngari with reference to digestibility and antioxidant potential. The different heat processes significantly increased the dry matter content, thereby altering the proximate composition of the ngari. Nutritional analyses revealed that the highest value of protein is in air fried ngari (44.14%), while maximum fat in sautéed ngari (27.57%). Fatty acids analysis showed that palmitic, oleic and linoleic acid were the major fatty acids present in ngari irrespective of different heat processing. Antioxidant potential of ngari was significantly influenced by heating processes and digestion methods (pepsin or pepsin-cum-pancreatin). Heat processes significantly reduced DPPH (2,2-diphenyl-1-picrylhydrazyl) and metal chelating activity, while increasing the reducing power of the nagri. Study on peptide released during digestion by SDS-PAGE (Sodium lauryl sulphate-polyacrylamide gel electrophoresis) showed that pepsin digestion led to formation of low molecular weight peptides (14-66 kDa). Subsequently, the pepsin-cum-pancreatin digestion degraded the relatively larger peptides into further smaller ones. The different heat processes enhanced the sensory appeal of the product without exerting any negative influence on its digestibility and bioactive properties

Effects of Cooking Methods and in-vitro Digestion on the Digestibility and Antioxidant Properties of Ngari (a fermented fish product of India)

Ngari is a popular ethnic fermented fish product from North-Eastern India. It is consumed after heat process either as a part of regular meal or as a condiment. However, there is no adequate knowledge on the digestion of Ngari and the bioactive potential of the digest. The present study was aimed to evaluate the effect of different heat processes viz., air frying, roasting and sautéing-on the chemical attributes and in-vitro digestion characteristics of Ngari with reference to digestibility and antioxidant potential. The different heat processes significantly increased the dry matter content, thereby altering the proximate composition of the Ngari. Nutritional analyses revealed that highest value of protein is in air fried Ngari (44.14%), while maximum fat in sautéed Ngari (27.57%). Fatty acids analysis showed that palmitic, oleic and linoleic acid were the major fatty acids present in Ngari irrespective of different heat processing. Antioxidant potential of Ngari was significantly influenced by heating processes and digestion methods (pepsin or pepsin-cum-pancreatin). Heat processes significantly reduced DPPH (2,2-diphenyl-1-picrylhydrazyl) and metal chelating activity, while increasing the reducing power of the Nagri. Study of peptide released during digestion by SDS-PAGE (Sodium lauryl sulphate-polyacrylamide gel electrophoresis) showed that pepsin digestion led to formation of low molecular weight peptides (14-66 kDa). Subsequently, the pepsin-cum-pancreatin digestion disintegrated the relatively larger peptides into further smaller ones. The different heat processes enhanced the sensory appeal of the product without exerting any negative influence on its digestibility and bioactive properties

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Not AvailableFermented fish products are very popular in North East region of India because of its unique taste and flavour. Two popular products, puthi shidal and phasa shidal, from Tripura were analyzed for biogenic amines, toxic heavy metals and foodborne micro-organisms of public health significance viz., Salmonella, E. coli and Staphylococcus aureus. Biogenic amines were detected by HPLC with UV-VIS detector using gradient elution method. Highest concentration of histamine, cadaverine and putrescine were detected in puthi shidal collected from Nutanbazar with a value of 118.18 plus or minus 1.78 ppm, 380.26 plus or minus 2.15 ppm and 141.87 plus or minus 1.20 ppm respectively. Spermidine and spermine were detected to be in the range of 21.38 plus or minus 0.88 to 41.89 plus or minus 0.83 ppm and 47.99 plus or minus 0.65 to 124.50 plus or minus 0.87 ppm respectively which was comparatively at lower concentration. Arsenic (As), cadmium (Cd) mercury (Hg) and lead (Pb) were determined using ICP-OES. The concentrations of heavy metals detected were within maximum permissible limit. Arsenic concentration of 2.65 plus or minus 0.02 ppm was recorded as maximum level in puthi shidal from Gulbazar (West Tripura district). Shidal samples are found to be free from pathogen like Salmonella and E. coli. The study indicates that the fermented fish products are free from heavy metal contaminations. Pathogen free fermented fish products are safe to consume. The study further revealed the need for standard production method in order to achieve uniform sensory attributes in the products including the control on histamine formation.Not Availabl