6 research outputs found
Enzymatic digestion improves testicular sperm retrieval in non-obstructive azoospermic patients
Background: In patients with non-obstructive azoospermia (NOA), vital
spermatozoa from the tissue is obtained from testes by enzymatic
treatment besides the mechanical treatment. Objective: To increase the
sperm recovery success of testicular sperm extraction (TESE), with
enzymatic digestion if no sperm is obtained from testis tissue by
mechanical method. Materials and Methods: Tissue samples were collected
from 150 men who presented with clinical and laboratory data indicating
NOA by means of TESE and micro dissection TESE methods. Initially,
mature spermatozoa were examined for by mechanical extraction technique
shredding the biopsy fractions. In cases whom no spermatozoa was
observed after maximum 30 min of initial searching under the inverted
microscope, the procedure was followed by enzymatic digestion using
DNaseI and collagenase type IV. Surgery type, pathology, AZF,
karyotype, hormones and testis size were compared in patients. Results:
Of 150 cases with NOA, conventional mincing method extended with
enzymatic treatment yielded successful sperm recovery in 13 (about 9%)
patients. Comparison of parameters revealed that level of FSH and LH
were significantly different (p=0.04 and 0.08 respectively) between two
groups that response negative and positive to enzymatic digestion.
Conclusion: The combination of conventional TESE and enzymatic
digestion is an effective method to recover spermatozoa. The benefit of
the mincing combined with enzyme to sperm retrieval for NOA firstly
shorten the mechanical searching time, leading to minimizing further
cellular damage as well as exposure to external conditions, and
secondly reduce the number of cases with sperm recovery failures. Also,
the serum level of FSH and LH are factors that influence the chance of
sperm retrieval
Predictive Factors of Successful Microdissection Testicular Sperm Extraction in Patients with Presumed Sertoli Cell-Only Syndrome
Background: To evaluate predictive factors of successful microdissection-testicular
sperm extraction (MD-TESE) in patients with presumed Sertoli cell-only syndrome
(SCOS).
Materials and Methods: In this retrospective analysis, 874 men with non-obstructive
azoospermia (NOA), among whom 148 individuals with diagnosis of SCOS in prior
biopsy, underwent MD-TESE at Department of Andrology, Royan Institute, Tehran, Iran.
The predictive values of follicle stimulating hormone (FSH), luteinizing hormone (LH),
and testosterone (T) levels, testicular volume, as well as male age for retrieving testicular
sperm by MD-TESE were analyzed by multiple logistic regression analysis.
Results: Testicular sperm were successfully retrieved in 23.6% men with presumed
SCOS. Using receiver operating characteristic (ROC) curve analysis, it was shown that
sperm retrieval rate in the group of men with FSH values >15.25% was 28.9%. This was
higher than the group of men with FSH ≤15.25 (11.8%).
Conclusion: Sperm retrieval rate (SRR) was 23.6% in men with presumed SCOS and
FSH level can be a fair predictor for SPR at MD-TESE. MD-TESE appears to be recommendable
in such cases (SCOS with high FSH concentration) with reasonable results.
Keywords: Follicle Stimulating Hormone, Luteinizing Hormone, Sperm Retrieval, Azoospermia,
Nonobstructiv