Protective effects of antiâ C5a peptide antibodies in experimental sepsis

We evaluated antibodies to different peptide regions of rat C5a in the sepsis model of cecal ligation and puncture (CLP) for their protective effects in rats. Rabbit polyclonal antibodies were developed to the following peptide regions of rat C5a: aminoâ terminal region (A), residues 1â 16; middle region (M), residues 17â 36; and the carboxylâ terminal region (C), residues 58â 77. With rat neutrophils, the chemotactic activity of rat C5a was significantly inhibited by antibodies with the following rank order: antiâ C > antiâ M â « antiâ A. In vivo, antibodies to the M and C (but not A) regions of C5a were protective in experimental sepsis, as determined by survival over a 10â day period, in a doseâ dependent manner. The relative protective efficacies of antiâ C5a preparations (in descending order of efficacy) were antiâ C â ¥ antiâ M â « antiâ A. In CLP rats, a delay in infusion of antibodies, which were injected at 6 or 12 h after CLP, still resulted in significant improvement in survival rates. These in vivo and in vitro data suggest that there are optimal targets on C5a for blockade during sepsis and that delayed infusion of antiâ C5a antibody until after onset of clinical evidence of sepsis still provides protective effects.Peer Reviewedhttps://deepblue.lib.umich.edu/bitstream/2027.42/154417/1/fsb2fj000653fje-sup-0001.pdfhttps://deepblue.lib.umich.edu/bitstream/2027.42/154417/2/fsb2fj000653fje.pdfhttps://deepblue.lib.umich.edu/bitstream/2027.42/154417/3/fsb2fj000653fje-sup-0002.pd

Large scale field trial in a landfill cover system with veneer reinforcement

Landfill cover systems are often composed of the succession of different soils and geosynthetics serving specific functions. When the systems are laid on slopes, high tensile forces are generated by the weight of the top soil that tends to move the system downwards. The use of high strength geogrids is nowadays a well-established technology that enables the safe construction of such slopes and limit the tensile force transfer to the sealing system. To better investigate the behavior of the geogrid in such conditions, a field trial was carried out in a municipal solid waste (MSW) landfill cover system with geosynthetics. In the large scale trial, the geogrid behavior was analyzed in terms of deformation during different phases: installation, monitoring and controlled failure. Different displacement transducers were installed in the geogrid along the slope length and the anchor trench. The system was continuously monitored during one month after construction and after that it was brought to a controlled failure. In this paper, the setup of the field trial and the preliminary results during installation, monitoring and failure of the system are presented and discussed

Validation of an intrinsic groundwater pollution vulnerability methodology using a national nitrate database.

The importance of groundwater for potable supply, and the many sources of anthropogenic contamination, has led to the development of intrinsic groundwater vulnerability mapping. An Analysis of Co-Variance and Analysis of Variance are used to validate the extensively applied UK methodology, based upon nitrate concentrations from 1,108 boreholes throughout England and Wales. These largely confirm the current aquifer and soil leaching potential classifications and demonstrate the benefits of combining soil and low permeability drift information. European legislation such as the Water Framework Directive will require more dynamic assessments of pollutant risk to groundwater. These results demonstrate that a number of improvements are required to future intrinsic groundwater vulnerability methodologies. The vertical succession of geological units must be included, so that non-aquifers can be zoned in the same way as aquifers for water supply purposes, while at the same time recognising their role in influencing the quality of groundwater in deeper aquifers. Classifications within intrinsic vulnerability methodologies should be based upon defined diagnostic properties rather than expert judgement. Finally the incorporation into groundwater vulnerability methodologies of preferential flow in relation to geological deposits, soil type and land management practices represents a significant, but important, future challenge

IOC consensus statement: "Training the elite child athlete"

Protecting the health of the athlete is the primary goal of the International Olympic Committee’s Medical Commission. One of its main objectives is the promotion of safe practices in the training of the elite child athlete. The elite child athlete is one who has superior athletic talent, undergoes specialized training, receives expert coaching and is exposed to early competition. Sport provides a positive environment that may enhance the physical growth and psychological development of children. This unique athlete population has distinct social, emotional and physical needs which vary depending on the athlete’s particular stage of maturation. The elite child athlete requires appropriate training, coaching and competition that ensure a safe and healthy athletic career and promote future well-being. This document reviews the scientific basis of sports training in the child, the special challenges and unique features of training elite children and provides recommendations to parents, coaches, health care providers, sports governing bodies and significant other parties