6 research outputs found
Comparative Analysis of the Transcriptome in Tissues Secreting Purple and White Nacre in the Pearl Mussel <em>Hyriopsis cumingii</em>
<div><p>The triangle sail mussel <em>Hyriopsis cumingii</em> (<em>Lea</em>) is the most important mussel species used for commercial freshwater pearl production in China. Mussel color is an important indicator of pearl quality. To identify genes involved in the nacre coloring, we conducted RNA-seq and obtained 541,268 sequences (298 bp average size) and 440,034 sequences (293 bp average size) in secreting purple and white nacre libraries (P- and W-libraries), respectively. The 981,302 Expressed Sequence Tags (ESTs) were assembled into 47,812 contigs and 289,386 singletons. In BLASTP searches of the deduced protein, 22,495 were proteins with functional annotations. Thirty-three genes involved in pearl or shell formation were identified. Digital expression analysis identified a total of 358 differentially expressed genes, and 137 genes in the P-library and 221 genes in the W-library showed significantly higher expression. Furthermore, a set of SSR motifs and SNPs between the two samples was identified from the ESTs, which provided the markers for genetic linkage, QTL analysis and future breeding. These EST sequences provided valuable information to further understand the molecular mechanisms involved in the formation, color determination and evolution of the pearl or shell.</p> </div
Schematic of deduced proteins contained tandem-arranged repeat units (1–5) enriched Asp residues, (6–9) enriched Lys residues, (10–18) enriched Gly residues.
<p>Each motif and copy number was shown on the right from up to down. Bars = 50 amino acids.</p
Gene Ontology classification of deduced protein sequences.
<p>Gene Ontology classification of deduced protein sequences.</p
Genes involved in the biomineralization process.
<p>Genes involved in the biomineralization process.</p
COG classification of deduced protein sequences.
<p>COG classification of deduced protein sequences.</p