Sex-specific competition differently regulates ecophysiological responses and phytoremediation of Populus cathayana under Pb stress

Lead (Pb) contamination seriously threatens agroforestry production and safety. We aim to determine the interactive influence of Pb and sexual competition on the growth performance, photosynthetic and biochemical traits, ultrastructure and phytoremediation-related parameters of males and females. In the present study, eco-physiological responses and phytoremediation traits of Populus cathayana females and males were evaluated under interactive treatments of Pb and competition. There were significant sex-specific competition effects on biomass partition, photosynthetic activities, carbohydrate contents, nitrogen and phosphorus use efficiencies, ultrastructure and phytoremediation under Pb stress. When competition within the same sex was compared, females were more sensitive to Pb stress, while males possessed greater Pb contents, and a higher bioconcentration factor and tolerance index. Under inter-sexual competition, males alleviated competition effects through greater Pb absorption, and lower photosynthetic rates, nutrient use efficiencies and biomass accumulation. Moreover, Pb stress altered competition intensities of both sexes. Sex-specific competition and neighbor effects may regulate responses and phytoremediation under heavy metal stress in dioecious plants. In the future, more attention should be paid on the effects of inter- and intra-sexual competition on dioecious species in the process of forestation and restoration of contaminated soil.Peer reviewe

Rootstock determines the drought resistance of poplar grafting combinations

To increase yield and/or enhance resistance to diseases, grafting is often applied in agriculture and horticulture. Interspecific grafting could possibly be used in forestry as well to improve drought resistance, but our understanding of how the rootstock of a more drought-resistant species can affect the grafted plant is very limited. Reciprocal grafts of two poplar species, Populus cathayana Rehder (less drought-resistant, C) and Populus deltoides Bart. ex Marsh (more drought-resistant, D) were generated. Four grafting combinations (scion/rootstock: C/C, C/D, D/D and D/C) were subjected to well-watered and drought stress treatments. C/D and D/C had a higher diameter growth rate, leaf biomass, intrinsic water-use efficiency (WUEi) and total non-structural carbohydrate (NSC) content than C/C and D/D in well-watered condition. However, drought caused greater differences between P. deltoides-rooted and P. cathayana-rooted grafting combinations, especially between C/D and D/C. The C/D grafting combination showed higher resistance to drought, as indicated by a higher stem growth rate, net photosynthetic rate, WUEi, leaf water potential, proline concentration and NSC concentration and maintenance of integrity of the leaf cellular ultrastructure under drought when compared with D/C. D/C exhibited severely damaged cell membranes, mitochondria and chloroplasts under drought. The scion genotype caused a strong effect on the root proline concentration: the P. cathayana scion increased the root proline concentration more than the P. deltoides scion (C/C vs D/C and C/D vs D/D) under water deficit. Our results demonstrated that mainly the rootstock was responsible for the drought resistance of grafting combinations. Grafting of the P. cathayana scion onto P. deltoides rootstock resulted in superior growth and biomass when compared with the other three combinations both in well-watered and drought stress conditions.Peer reviewe

Integrated DNA methylation, transcriptome and physiological analyses reveal new insights into superiority of poplars formed by interspecific grafting

Plant grafting has a long history and it is extensively employed to improve plant performance. In our previous research, reciprocal grafts of Populus cathayana Rehder (C) and Populus deltoides Bart. Ex Marsh (D) were generated. The results showed that interspecific grafting combinations (scion/rootstock: C/D and D/C) grew better than intraspecific grafting combinations (C/C and D/D). To further understand differences in molecular mechanisms between interspecific and intraspecific grafting, we performed an integrated analysis, including bisulfite sequencing, RNA sequencing and measurements of physiological indicators, to investigate leaves of different grafting combinations. We found that the difference at the genome-wide methylation level was greater in D/C vs D/D than in C/D vs C/C, but no difference was detected at the transcription level in D/C vs D/D. Furthermore, the grafting superiority of D/C vs D/D was not as strong as that of C/D vs C/C. These results may be associated with the different methylation forms, mCHH (71.76%) and mCG (57.16%), that accounted for the highest percentages in C/D vs C/C and D/C vs D/D, respectively. In addition, the interspecific grafting superiority was found mainly related to the process of photosynthesis, phytohormone signal transduction, biosynthesis of secondary metabolites, cell wall and transcriptional regulation based on both physiological and molecular results. Overall, the results indicated that the physiological and molecular phenotypes of grafted plants are affected by the interaction between scion and rootstock. Thus, our study provides a theoretical basis for developing suitable scion-rootstock combinations for grafted plants.Peer reviewe

Improved drought resistance by intergeneric graftingin Salicaceae plants under water deficits

In this study, intergeneric grafting was employed between Populus cathayana and Salix rehderiana to investigate the grafting compatibility of the two Salicaceae plants and to reveal whether grafting can improve their drought resistance. Under different grafting combinations (P. cathayana scion with P. cathayana rootstock, P/P; P. cathayana scion with S. rehderiana rootstock, P/S; S. rehderiana scion with S. rehderiana rootstock, S/S; and S. rehderiana scion with P. cathayana rootstock, S/P), the survival and growth rate, biomass accumulation and allocation, photosynthetic traits, carbon isotope composition (delta C-13), relative water content (RWC) and nonstructural carbohydrates (NSCs) were measured. The results showed that the grafting compatibility between P. cathayana and S. rehderiana was very high, as the survival rates ranged from 76% to 100% under different grafting combinations. Drought significantly decreased growth, biomass accumulation, photosynthetic pigment contents, net photosynthesis rates (P) and RWC, and increased delta C-13 in all grafting combinations. Under drought stress, biomass accumulation, total chlorophyll, transpiration rate (E) and P-n were higher in P/P and P/S than in S/S and S/P. Compared with P/P, the growth rate, biomass accumulation, root/aboveground ratio (R/A ratio), carotenoid, RWC, starch and total soluble sugar (TSS) of P/S were less affected by drought. The height growth rate (GRH), R/A ratio, carotenoid, chlorophyll a, total chlorophyll, WUEi and TSS of S/P were lower than those of S/S under water-limited conditions. Moreover, a principal component analysis indicated that P/S and S/S had higher drought resistance than P/P and S/P under water deficits. The used method allows combining specific advantageous traits from P. cathayana and S. rehderiana, which may be a highly useful tool to enhance drought resistance in the cultivation of Salicaceae plants.Peer reviewe

Characteristics of very fast transient currents in ultra high-voltage power system with hybrid reactive power compensation

Hybrid reactive power compensation (HRPC) consists of a stepped controlled shunt reactor (SCSR) and a series compensation (SC), which will find applications in future ultra high-voltage (UHV) power grids to resolve the problems due to the frequent change of reactive power and bulk power transmission. However, very fast transient currents (VFTCs) are inevitably generated during switching, which would lead to insulation breakdown. In the present work, we first develop the equivalent model for HRPC, following which we deduce the expression of VFTCs in the time domain by using an inverse Laplace transform. The analysis indicates that the amplitude and frequency of VFTCs are both affected by the capacitance of the SCSR and of the SC, as well as the line length, stray capacitance, etc. The oscillating frequency, peak, and amplitude of the main frequency of the VFTCs in the substation can be modified by adjusting the silicon-controlled rectifiers in the SCSR when the disconnecting switch in gas-insulated switchgear is switched on. When the disconnecting switch in the SC is switched on, the VFTC oscillation frequency in SCSR decreases with increasing stray capacitance of SC, but the frequency and peak of the VFTC remains quite large. Increasing the line length between the SC and the SCSR suppresses the VFTC in UHV power systems. These results lay the foundation for developing HRPC methods to suppress VFTCs in UHV substations

Can material asset reorganizations affect acquirers’ debt financing costs? – Evidence from the Chinese Merger and Acquisition Market

In this paper, we investigate whether material asset reorganizations (MARs), a special form of merger and acquisition (M&A) transactions, can affect the acquirers’ cost of debt financing. Further, we examine the effect of acquiring firms’ accounting information quality on the cost of debt and on the association between MARs and debt costs. We predict that compared to conventional M&As, large-scale acquisitions through MARs can generate a much greater influx of assets from target firms. This raises the acquirers’ asset collateral and thus reduces the cost of debt. Because the quality of accounting information is a key factor affecting the cost of debt, we suggest that it has a spillover effect on the debt-cost effect of MARs. Using M&A transactions by listed companies in the Chinese A-share market from 2008 to 2014 as our sample, we find that MARs are associated with a higher asset collateral and lower ex post cost of debt than conventional M&As. Furthermore, we show that the acquiring firms’ accounting information quality has a significant negative effect on debt costs, and the negative association between MARs and the cost of debt is more pronounced when accounting information quality is higher. Keywords: Material asset reorganizations, Merger and acquisition, Accounting information quality, Asset collateral, Cost of debt financin

An E-type Temperature Sensor for Upper Air Meteorology

ABSTRACT: An E-type high-precision temperature sensor, which is adopted for upper air meteorology, was proposed in this paper. A computational fluid dynamics (CFD) method was implemented to analyze temperature rise induced by solar radiation at different altitudes and solar radiation intensities. A temperature rise correction equation was obtained by fitting the CFD results using a Broyden-Fletcher-Goldfarb-Shanno (BFGS) method. To verify the performance of the temperature sensor, an experimental platform was constructed. Through simulations and experiments, the relationship among the altitude, solar radiation intensity and radiation temperature rise was obtaned. The root-mean-square error (RMSE) between the temperature rise derived from the correction equation and that derived from the experiments is 0.013 K. The sample determination coefficient r2 of the solar radiation error correction equation is 0.9975. Keywords: High-precision temperature sensor, Computational fluid dynamics, Radiation temperatur

An Advanced Partial Discharge Recognition Strategy of Power Cable

Detection and localization of partial discharge are very important in condition monitoring of power cables, so it is necessary to build an accurate recognizer to recognize the discharge types. In this paper, firstly, a power cable model based on FDTD simulation is built to get the typical discharge signals as training samples. Secondly, because the extraction of discharge signal features is crucial, fractal characteristics of the training samples are extracted and inputted into the recognizer. To make the results more accurate, multi-SVM recognizer made up of six Support Vector Machines (SVM) is proposed in this paper. The result of the multi-SVM recognizer is determined by the vote of the six SVM. Finally, the BP neural networks and ELM are compared with multi-SVM. The accuracy comparison shows that the multi-SVM recognizer has the best accuracy and stability, and it can recognize the discharge type efficiently