Quantifying and visualizing the transcranial direct current stimulation research indicators

The field of transcranial direct current stimulation (tDCS) has experienced significant growth in the past 15 years which is mainly devoted to determining the basic and clinical potential of tDCS in humans. The aim of this study is to quantitatively analyze the current worldwide progress on tDCS research as well as to highlight researchers, journals, institutions and countries which are contributing significantly in the past 18 years. We conducted a quantitative analysis of research articles regarding tDCS published from 1998 to 2016 and indexed in the web of science core collection database. Data was downloaded in October, 2016. In the past 18 years, there were 2457 studies on tDCS indexed by web of science database, including all documents type such as article, review, meeting abstract, proceedings paper, letters, and etc. This study is focusing on the main articles and reviews; therefore, the research production was reduced to 2000 publications. The analysis showed that most of the studies in the field were published by North American and European institutions with a reasonable proportion of the publications were also by Japanese institutions from Asia. From the perspective of research progress, we found that the number of published papers on tDCS has increased significantly in the past 10 years, between them a remarkable positive correlation exists

Interaction between bacterial endophytes and host plants

Endophytic bacteria are mainly present in the plant’s root systems. Endophytic bacteria improve plant health and are sometimes necessary to fight against adverse conditions. There is an increasing trend for the use of bacterial endophytes as bio-fertilizers. However, new challenges are also arising regarding the management of these newly discovered bacterial endophytes. Plant growth-promoting bacterial endophytes exist in a wide host range as part of their microbiome, and are proven to exhibit positive effects on plant growth. Endophytic bacterial communities within plant hosts are dynamic and affected by abiotic/biotic factors such as soil conditions, geographical distribution, climate, plant species, and plant-microbe interaction at a large scale. Therefore, there is a need to evaluate the mechanism of bacterial endophytes’ interaction with plants under field conditions before their application. Bacterial endophytes have both beneficial and harmful impacts on plants but the exact mechanism of interaction is poorly understood. A basic approach to exploit the potential genetic elements involved in an endophytic lifestyle is to compare the genomes of rhizospheric plant growth-promoting bacteria with endophytic bacteria. In this mini-review, we will be focused to characterize the genetic diversity and dynamics of endophyte interaction in different host plants

The Lytic SA Phage Demonstrate Bactericidal Activity against Mastitis Causing Staphylococcus aureus

Staphylococcus aureus is the major causative agent of mastitis among dairy animals as it causes intramammary gland infection. Due to antibiotic resistance and contamination of antibiotics in the milk of diseased animals; alternative therapeutic agents are required to cure mastitis. Lytic bacteriophages and their gene products can be potential therapeutic agents against bacteria as they are host specific and less harmful than antibiotics. In this study, Staphylococcus aureus were isolated from milk samples of the infected animals and identified biochemically. SA phage was isolated from sewage water showing lytic activity against Staphylococcus aureus isolates. The highest lytic activity of bacteriophages was observed at 37°C and pH 7, and the most suitable storage condition was at 4°C. SA phage efficiently reduced bacterial growth in the bacterial reduction assay. The characterization and bacterial growth reduction activity of the bacteriophages against Staphylococcus aureus signifies their underlying potential of phage therapy against mastitis

Seasonal And Regional Effects of Air Quality Index on Hematological Indices of Dogs Under Local Environmental Conditions In Pakistan

Air pollution is found to have significant association with living health all over global world. Environment Protection Department of Punjab, Pakistan provides the monthly air quality index (AQI) data on air pollution with concentrated particulates like PM2.5, PM10, NO2, SO2 and O3. Air particulates concentrations may vary of season, regional geography and climate. We performed blood sampling of 45 dogs from different breeds (Labrador retrievers, German shepherds and Pit bulls) from three different areas categorized on basis of AQI as less polluted (Gulberg), polluted (Town Hall) and highly polluted (Shadman) in winter and spring seasons. Data were analysed using paired sample t-tests for seasonal study and independent sample t-tests for area and breed study by SPSS (P < 0.05 ascertained as significant). Seasonal study resulted that less polluted area had no significant effects on hematological indices in any three breeds of dogs in both seasons. Polluted area was presented with increased significant effects on values of monocytes in Labrador retrievers, Hct and MCHC in German shepherds while decreased significant effects on MCH value in Pit bulls in winter season as compared to spring season. Highly polluted area had significant effects with decreasing WBC counts only in German shepherds in winter season than spring season. This study concludes that polluted and highly polluted areas in winter season with worst AQI affects blood indices more than spring season in dogs

Coupling CRISPR/Cas9 and lambda red recombineering system for genome editing of <i>Salmonella</i> Gallinarum and the effect of <i>ssaU</i> knock-out mutant on the virulence of bacteria

Funding: This work was carried out with financial support from UK government, Department of Health and Social Care (DHSC), Global AMR Innovation Fund (GAMRIF) and the International Development Research Centre Ottawa, Canada, (Grant No. 109051-002).The poultry industry in developing countries still faces a significant threat from fowl typhoid, a disease caused by Salmonella Gallinarum that has been well contained in more economically developed countries. In addition to the virulence exhibited by large virulence plasmid (85 kb), Salmonella Pathogenicity Island 2 in S. Gallinarum plays a key role in mediating disease through its type III secretion systems (TTSS). The TTSS secrete effector protein across the Salmonella containing vacuoles and mediate the internalization of bacteria by modulating vesicular passage. In this study, candidate virulent ssaU gene (~1 kb) encoding type III secretion system was successfully deleted from indigenously isolated S. Gallinarum genome through homology-directed repair using CRISPR/Cas9 and lambda recombination systems. CRISPR/Cas9-based genome editing of poultry-derived Salmonella Gallinarum has not been previously reported, which might be linked to a lack of efficiency in its genetic tools. This is the first study which demonstrates a complete CRISPR/Cas9-based gene deletion from this bacterial genome. More importantly, a poultry experimental model was employed to assess the virulence potential of this mutant strain (ΔssaU_SG18) which was unable to produce any mortality in the experimentally challenged birds as compared to the wild type strain. No effect on weight gain was observed whereas bacteria were unable to colonize the intestine and liver in our challenge model. This in vivo loss of virulence in mutant strain provides an excellent functionality of this system to be useful in live vaccine development against this resistant and patho genic bacteria.Publisher PDFPeer reviewe

Feminism in Pakistan and Emerging Perspectives

Since March 2019, local NGOs in Pakistan are organizing “Aurat March” (Women March) in month of March to raise voice against indigenous feminine issues and violence against women. In hindu-originated society like Pakistan, where Islamic women rights haven’t implemented in its true spirit yet, digesting such a “liberal” act is not easily digestible. Thus different intellectual classes took social media on fire to outdo each other and central topic of cross arguments was slogans raised in the March. Raising slogans is a traditional way of weaker class in society to demonstrate against discrimination and emit emotional suffocation. To study underlying meanings of much debated slogans of Aurat March in Pakistan, discourse analysis is employed which also facilitated to indicate emerging dimension socio-religious debates and opinions towards feminism in Pakistan