Wound healing and analgesic effects of Brocchia cinerea essential oil in experimental animals

This study aimed to identify the main components of the essential oil extracted from Brocchia cinerea (Delile) Vis. via hydrodistillation and investigate its in vivo wound healing and analgesic properties. Thujone, santolina triene, camphor, and 1,8-cineole were among the compounds detected. Wounds were induced in mice and treated with essential oil, which resulted in accelerated wound healing and repair through topical application (88.1±1.1%). The study also evaluated the analgesic activity of the essential oil by administering intraperitoneal injections of acetic acid to mice. The results showed that B. cinerea essential oil at a dose of 400 mg/kg strongly inhibited pain, with a pain inhibition percentage of 95.5%. These findings indicate that the essential oil of B. cinerea has potential as a source of bioactive compounds that may have synergistic effects. Based on these results, the use of B. cinerea for therapeutic purposes in preventing pain and promoting wound healing is supported. These findings highlight the potential of B. cinerea in paving the way for future research aimed at the development of clinically valuable products

Effect of extraction technique on the content and antioxidant activity of crude extract of <i>Anacyclus clavatus</i> flowers and their essential oil composition

<div><p><i>Anacyclus clavatus</i> is a plant used as food and remedy. The objective of this work was to study the effect of extraction technique on the antioxidant property, total phenol and flavonoid contents of crude extracts from <i>A. clavatus</i> flowers and their essential oil composition. 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay, ferric-reducing power, β-carotene and total antioxidant capacity assays have demonstrated the significant antioxidant ability of different crude extracts obtained by using the following extraction methods: Soxhlet, microwave heating, heat reflux (HRE) and maceration. The activity of the extract obtained by HRE was the highest (112.06 ± 2.89 μg/mL) evaluated by the DPPH assay. Extraction of essential oil was performed by microwave-assisted hydro-distillation (MAHD) and by hydro-distillation (HD). A significant difference was observed in both essential oils, despite the common main family and major constituents, such as artemisia ketone (10.0 ± 0.8% for MAHD vs. 6.5 ± 0.5 for HD) and pinocarvone (4.1 ± 0.4% for MAHD vs. 1.1 ± 0.1% for HD).</p></div