P66shc and its downstream Eps8 and Rac1 proteins are upregulated in esophageal cancers

Members of Shc (src homology and collagen homology) family, p46shc, p52shc, p66shc have known to be related to cell proliferation and carcinogenesis. Whereas p46shc and p52shc drive the reaction forward, the role of p66shc in cancers remains to be understood clearly. Hence, their expression in cancers needs to be evaluated carefully so that Shc analysis may provide prognostic information in the development of carcinogenesis. In the present study, the expression of p66shc and its associate targets namely Eps8 (epidermal pathway substrate 8), Rac1 (ras-related C3 botulinum toxin substrate1) and Grb2 (growth factor receptor bound protein 2) were examined in fresh tissue specimens from patients with esophageal squamous cell carcinoma and esophageal adenocarcinoma using western blot analysis. A thorough analysis of both esophageal squamous cell carcinoma and adenocarcinoma showed p66shc expression to be significantly higher in both types of carcinomas as compared to the controls. The controls of adenocarcinoma show a higher basal expression level of p66shc as compared to the controls of squamous cell carcinoma. The expression level of downstream targets of p66shc i.e., eps8 and rac1 was also found to be consistently higher in human esophageal carcinomas, and hence correlated positively with p66shc expression. However the expression of grb2 was found to be equal in both esophageal squamous cell carcinoma and adenocarcinoma. The above results suggest that the pathway operated by p66shc in cancers does not involve the participation of Ras and Grb2 as downstream targets instead it operates the pathway involving Eps8 and Rac1 proteins. From the results it is also suggestive that p66shc may have a role in the regulation of esophageal carcinomas and represents a possible mechanism of signaling for the development of squamous cell carcinoma and adenocarcinoma of esophagus

Mushroom refinement endeavor auspicate non green revolution in the offing

Pala SA, Wani AH, Boda RH, Wani BA. 2014. Mushroom refinement endeavor auspicate non green revolution in the offing. Nusantara Bioscience 6: 173-185. Mushroom can serve as food, tonic, and as medicine thus make people healthier, fitter and happier. They have a cracking potential for generating great socioeconomic impact in human welfare at local, national and international level. With the help of allied mushroom farming we can easily tackle the problem of food for growing world population; reduce environmental pollution by bioconversion of huge organic wastes into mushrooms; recycle huge quantity of organic wastes to mushroom crops, biofertilizers, and biogas; restore damaged environment by mushroom mycelia through mycoforestry, mycoremediation, mycofiltration and mycopesticides in a zero emission fashion. They can be used to degrade radioactive industrial biocide wastes in an eco-friendly fashion. Since mushroom cultivation is an indoor agribusiness, it could have great economic impact by generating employment, income and functional food requirements for rural people especially in developing countries. How far mushroom cultivation can meet the functional food requirements; address the domestic food challenges, rising food prices and crisis vis a vis environmental sustainability will be thrust areas of this communication

Multi-Scale Biomechanical Remodeling in Aging and Genetic Mutant Murine Mitral Valve Leaflets: Insights into Marfan Syndrome

<div><p>Mitral valve degeneration is a key component of the pathophysiology of Marfan syndrome. The biomechanical consequences of aging and genetic mutation in mitral valves are poorly understood because of limited tools to study this in mouse models. Our aim was to determine the global biomechanical and local cell-matrix deformation relationships in the aging and Marfan related <em>Fbn1</em> mutated murine mitral valve. To conduct this investigation, a novel stretching apparatus and gripping method was implemented to directly quantify both global tissue biomechanics and local cellular deformation and matrix fiber realignment in murine mitral valves. Excised mitral valve leaflets from wild-type and <em>Fbn1</em> mutant mice from 2 weeks to 10 months in age were tested in circumferential orientation under continuous laser optical imaging. Mouse mitral valves stiffen with age, correlating with increases in collagen fraction and matrix fiber alignment. <em>Fbn1</em> mutation resulted in significantly more compliant valves (modulus 1.34±0.12 vs. 2.51±0.31 MPa, respectively, P<.01) at 4 months, corresponding with an increase in proportion of GAGs and decrease in elastin fraction. Local cellular deformation and fiber alignment change linearly with global tissue stretch, and these slopes become more extreme with aging. In comparison, <em>Fbn1</em> mutated valves have decoupled cellular deformation and fiber alignment with tissue stretch. Taken together, quantitative understanding of multi-scale murine planar tissue biomechanics is essential for establishing consequences of aging and genetic mutations. Decoupling of local cell-matrix deformation kinematics with global tissue stretch may be an important mechanism of normal and pathological biomechanical remodeling in valves.</p> </div

Temporal biomechanical analysis of C57/B6J mitral valves.

<p>(A) Representative stress-strain curves of mitral valves loaded in the circumferential direction (not to failure). (B) Stress-strain data were fit to an exponential Fung model, from which coefficients were used to determine effective modulus. (C) Representative circularity index curves as defined by the ratio of 4*Pi*(Area/Perimeter²). (D) Circularity-index curves were modeled as a linear fit and the negative slope was used for comparison. (E) Representative fiber-alignment curves were defined by the Fourier-Transform of collagen alignment and summed within ten degrees of the image horizontal, which was parallel to the tissue and loading direction. (F) Fiber-alignment data were fit to a linear model, the negative slope of which was used for comparison. Error bars show ±SD, n ≥6 for each condition. Groups that do not share letters are significantly different from each other according to a one-way ANOVA with Tukey’s post hoc (p≤0.05).</p

Differences between ^+/+<i>Fbn1</i> and ^C1039G/+<i>Fbn1</i> mitral valve matrix composition at 4 months.

<p>(A–B) Masson’s Trichrome reveals a reduction in the fractional amount of connective tissue in the marfan mitral valve compared to wildtype. (C) Digital quantification of the connective tissue composition using color thresholding, with blue regions denoting connective tissue. (D–F) Movat’s stain reveals over 3-fold reduction in the fractional amount of collagen compared to GAGs in the Marfan mitral valve relative to the wildtype (yellow-collagen, blue-GAGs). (G–I) Verhoeff’s–van Gieson (VVG) stain reveals a significant reduction in the fractional amount of elastin in the Marfan mitral valve compared to the wildtype (purple/black-elastin). Magnification, ×4. Scale bars: 200 µm. Error bars show ±SD, n ≥3 valves per condition. Asterisks denote statistical differences according to a Student’s t-test (p≤0.05).</p

Temporal histological examination of C57/B6J mitral valves.

<p>(A) 2 week old murine mitral valves contain similar fractional amounts of collagen and GAGs, with the majority of collagen near the attachment zones but mostly undefined architecture (Movat’s stain: yellow/orange = collagen, green/blue = GAGs). (B) More collagen relative to GAGs is present in 3 week old MV, with matrix stratification developing (Arrows). (C) At 4 months, murine mitral valves have nearly 3 times the amount of collagen to GAGs, are significantly more compact, and have well defined atrialis/fibrosa strata (Arrows). (D) At 12 months, murine mitral valves have significantly less collagen to GAGs, with dramatically increased thickening and reduced structural organization. (E) Digital quantification of matrix composition using color thresholding. Data compared as the ratio of collagen to GAGs within each valve leaflet. Magnification, ×4. Scale bars: 200 µm. Error bars show ±SD, n ≥3 valves per time point. Bars that do not share any letters are significantly different according to a one-way ANOVA with Tukey’s post hoc (p≤0.05).</p

Biomechanical analysis of ^+/+<i>Fbn1</i> and ^C1039G/+<i>Fbn1</i> at 4 months.

<p>(A) Representative stress-strain responses of mitral valves loaded in the circumferential direction. (B) Stress-strain data were fit to an exponential Fung model, coefficients of which were used to determine effective modulus. (C) Representative stretch induced cell shape changes responses defined by the circularity index (CI) = 4*Pi*(Area/Perimeter²). (D) Circularity-index data were modeled as a linear fit, the negative slope of which was used for comparison. Error bars show ±SD, n ≥6 valves per condition. Asterisks signify statistical differences according to a Student’s t-test (p≤0.05).</p

Macro and micro-scale tissue analysis.

<p>(A–D) Macro scale valve deformation at varying stretch ratios (λ) viewed at 10× under confocal microscopy (Green: 5-DTAF stained matrix fibers, Red: vital dye labeled cell bodies). Stars denote centroid location of the cantilever posts. (E–H) Micro-scale cellular deformation of the same group of cells viewed during the same test at 40X under confocal microscopy. Arrows denote tracked morphology of individual cells. (I–L) Micro-scale fiber alignment from the same region viewed during the same test at 40× under confocal microscopy. Fiber un-crimping and alignment is clearly visible as stretch progresses left to right.</p