50 research outputs found
Presence of pathogenicity island genes in Enterococcus faecalis isolated from pigs in Denmark
Prevalence and characterization of plasmids carrying sulfonamide resistance genes among <em>Escherichia coli</em> from pigs, pig carcasses and human
BACKGROUND: Sulfonamide resistance is very common in Escherichia coli. The aim of this study was to characterize plasmids carrying sulfonamide resistance genes (sul1, sul2 and sul3) in E. coli isolated from pigs and humans with a specific objective to assess the genetic diversity of plasmids involved in the mobility of sul genes. METHODS: A total of 501 E. coli isolates from pig feces, pig carcasses and human stools were tested for their susceptibility to selected antimicrobial. Multiplex PCR was conducted to detect the presence of three sul genes among the sulfonamide-resistant E. coli isolates. Fifty-seven sulfonamide-resistant E. coli were selected based on presence of sul resistance genes and subjected to conjugation and/or transformation experiments. S1 nuclease digestion followed by pulsed-field gel electrophoresis was used to visualize and determine the size of plasmids. Plasmids carrying sul genes were characterized by PCR-based replicon typing to allow a comparison of the types of sul genes, the reservoir and plasmid present. RESULTS: A total of 109/501 isolates exhibited sulfonamide resistance. The relative prevalences of sul genes from the three reservoirs (pigs, pig carcasses and humans) were 65%, 45% and 12% for sul2, sul1, and sul3, respectively. Transfer of resistance through conjugation was observed in 42/57 isolates. Resistances to streptomycin, ampicillin and trimethoprim were co-transferred in most strains. Class 1 integrons were present in 80% of sul1-carrying plasmids and 100% of sul3-carrying plasmids, but only in 5% of sul2-carrying plasmids. The sul plasmids ranged from 33 to 160-kb in size and belonged to nine different incompatibility (Inc) groups: FII, FIB, I1, FIA, B/O, FIC, N, HI1 and X1. IncFII was the dominant type in sul2-carrying plasmids (52%), while IncI1 was the most common type in sul1 and sul3-carrying plasmids (33% and 45%, respectively). Multireplicons were found associated with all three sul genes. CONCLUSIONS: Sul genes were distributed widely in E. coli isolated from pigs and humans with sul2 being most prevalent. Sul-carrying plasmids belonged to diverse replicon types, but most of detected plasmids were conjugative enabling horizontal transfer. IncFII seems to be the dominant replicon type in sul2-carrying plasmids from all three sources
Development of a web tool for Escherichia coli subtyping based on fimH alleles:Running title: Development of E. coli fimH sub-typing web-tool
ABSTRACT
The aim of this study was to construct a valid publicly available method for
in silico fimH
subtyping of
Escherichia coli
particularly suitable for differentiation of fine-resolution subgroups within clonal groups defined by standard multilocus sequence typing (MLST). FimTyper was constructed as a FASTA database containing all currently known
fimH
alleles. The software source code is publicly available at
https://bitbucket.org/genomicepidemiology/fimtyper
, the database is freely available at
https://bitbucket.org/genomicepidemiology/fimtyper_db
, and a service implementing the software is available at
https://cge.cbs.dtu.dk/services/FimTyper
. FimTyper was validated on three data sets: one containing Sanger sequences of
fimH
alleles of 42
E. coli
isolates generated prior to the current study (data set 1), one containing whole-genome sequence (WGS) data of 243 third-generation-cephalosporin-resistant
E. coli
isolates (data set 2), and one containing a randomly chosen subset of 40
E. coli
isolates from data set 2 that were subjected to conventional
fimH
subtyping (data set 3). The combination of the three data sets enabled an evaluation and comparison of FimTyper on both Sanger sequences and WGS data. FimTyper correctly predicted all 42
fimH
subtypes from the Sanger sequences from data set 1 and successfully analyzed all 243 draft genomes from data set 2. FimTyper subtyping of the Sanger sequences and WGS data from data set 3 were in complete agreement. Additionally,
fimH
subtyping was evaluated on a phylogenetic network of 122 sequence type 131 (ST131)
E. coli
isolates. There was perfect concordance between the typology and
fimH
-based subclones within ST131, with accurate identification of the pandemic multidrug-resistant clonal subgroup ST131-
H
30. FimTyper provides a standardized tool, as a rapid alternative to conventional
fimH
subtyping, highly suitable for surveillance and outbreak detection.
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Danish Integrated Antimicrobial Resistance Monitoring and Research Program
This program has led to changes in the use of antimicrobial agents in Denmark and other countries