Ferromagnetic resonance force microscopy on a thin permalloy film

Ferromagnetic Resonance Force Microscopy (FMRFM) offers a means of performing local ferromagnetic resonance. We have studied the evolution of the FMRFM force spectra in a continuous 50 nm thick permalloy film as a function of probe-film distance and performed numerical simulations of the intensity of the FMRFM probe-film interaction force, accounting for the presence of the localized strongly nonuniform magnetic field of the FMRFM probe magnet. Excellent agreement between the experimental data and the simulation results provides insight into the mechanism of FMR mode excitation in an FMRFM experiment.Comment: 9 pages, 2 figure

Mid-Infrared Ethane Emission on Neptune and Uranus

We report 8- to 13-micron spectral observations of Neptune and Uranus from the NASA Infrared Telescope Facility spanning more than a decade. The spectroscopic data indicate a steady increase in Neptune's mean atmospheric 12-micron ethane emission from 1985 to 2003, followed by a slight decrease in 2004. The simplest explanation for the intensity variation is an increase in stratospheric effective temperature from 155 +/- 3 K in 1985 to 176 +/- 3 K in 2003 (an average rate of 1.2 K/year), and subsequent decrease to 165 +/- 3 K in 2004. We also detected variation of the overall spectral structure of the ethane band, specifically an apparent absorption structure in the central portion of the band; this structure arises from coarse spectral sampling coupled with a non-uniform response function within the detector elements. We also report a probable direct detection of ethane emission on Uranus. The deduced peak mole fraction is approximately an order of magnitude higher than previous upper limits for Uranus. The model fit suggests an effective temperature of 114 +/- 3 K for the globally-averaged stratosphere of Uranus, which is consistent with recent measurements indicative of seasonal variation.Comment: Accepted for publication in ApJ. 16 pages, 10 figures, 2 table

SFTA2 - a novel secretory peptide highly expressed in the lung - is modulated by lipopolysaccharide but not hyperoxia

Tissue-specific transcripts are likely to be of importance for the corresponding organ. While attempting to define the specific transcriptome of the human lung, we identified the transcript of a yet uncharacterized protein, SFTA2. In silico analyses, biochemical methods, fluorescence imaging and animal challenge experiments were employed to characterize SFTA2. Human SFTA2 is located on Chr. 6p21.33, a disease-susceptibility locus for diffuse panbronchiolitis. RT-PCR verified the abundance of SFTA2-specific transcripts in human and mouse lung. SFTA2 is synthesized as a hydrophilic precursor releasing a 59 amino acid mature peptide after cleavage of an N-terminal secretory signal. SFTA2 has no recognizable homology to other proteins while orthologues are present in all mammals. SFTA2 is a glycosylated protein and specifically expressed in nonciliated bronchiolar epithelium and type II pneumocytes. In accordance with other hydrophilic surfactant proteins, SFTA2 did not colocalize with lamellar bodies but colocalized with golgin97 and clathrin-labelled vesicles, suggesting a classical secretory pathway for its expression and secretion. In the mouse lung, Sfta2 was significantly downregulated after induction of an inflammatory reaction by intratracheal lipopolysaccharides paralleling surfactant proteins B and C but not D. Hyperoxia, however, did not alter SFTA2 mRNA levels. We have characterized SFTA2 and present it as a novel unique secretory peptide highly expressed in the lung

Self-field effects upon the critical current density of flat superconducting strips

We develop a general theory to account self-consistently for self-field effects upon the average transport critical current density Jc of a flat type-II superconducting strip in the mixed state when the bulk pinning is characterized by a field-dependent depinning critical current density Jp(B), where B is the local magnetic flux density. We first consider the possibility of both bulk and edge-pinning contributions but conclude that bulk pinning dominates over geometrical edge-barrier effects in state-of-the-art YBCO films and prototype second-generation coated conductors. We apply our theory using the Kim model, JpK(B) = JpK(0)/(1+|B|/B0), as an example. We calculate Jc(Ba) as a function of a perpendicular applied magnetic induction Ba and show how Jc(Ba) is related to JpK(B). We find that Jc(Ba) is very nearly equal to JpK(Ba) when Ba > Ba*, where Ba* is the value of Ba that makes the net flux density zero at the strip's edge. However, Jc(Ba) is suppressed relative to JpK(Ba) at low fields when Ba < Ba*, with the largest suppression occurring when Ba*/B0 is of order unity or larger.Comment: 9 pages, 4 figures, minor revisions to add four reference

Limits on Phase Separation for Two-Dimensional Strongly Correlated Electrons

From calculations of the high temperature series for the free energy of the two-dimensional t-J model we construct series for ratios of the free energy per hole. The ratios can be extrapolated very accurately to low temperatures and used to investigate phase separation. Our results confirm that phase separation occurs only for J/t greater than 1.2. Also, the phase transition into the phase separated state has Tc of approximately 0.25J for large J/t.Comment: 4 pages, 6 figure

On the Liaison Between Superconductivity and Phase Separation

Models of strongly correlated electrons that tend to phase separate are studied including a long-range 1/r repulsive interaction. It is observed that charge-density-wave states become stable as the strength of the 1/r term, ${\rm V_{coul}}$, is increased. Due to this effect, the domain of stability of the superconducting phases that appear near phase separation at ${\rm V_{coul} = 0}$ is not enlarged by a 1/r interaction as naively expected. Nevertheless, superconductivity exists in a wide region of parameter space, even if phase separation is suppressed. Our results have implications for some theories of the cuprates.Comment: 11 pages, 9 postscript figures are appende

Gliotoxin effects on fungal growth: Mechanisms and exploitation

Although initially investigated for its antifungal properties, little is actually known about the effect of gliotoxin on Aspergillus fumigatus and other fungi. We have observed that exposure of A. fumigatus to exogenous gliotoxin (14 lg/ml), under gliotoxin-limited growth conditions, results in significant alteration of the expression of 27 proteins (up- and down-regulated >1.9-fold; p < 0.05) including de novo expression of Cu, Zn superoxide dismutase, up-regulated allergen Asp f3 expression and down-regulated catalase and a peroxiredoxin levels. Significantly elevated glutathione GSH levels (p < 0.05), along with concomitant resistance to diamide, were evident in A. fumigatus ∆gliT, lacking gliotoxin oxidoreductase, a gliotoxin self-protection gene. Saccharomyces cerevisiae deletents (∆sod1 and ∆yap1) were hypersensitive to exogenous gliotoxin, while ∆gsh1 was resistant. Significant gliotoxin-mediated (5 µg/ml) growth inhibition (p < 0.001) of Aspergillus nidulans, Aspergillus terreus, Aspergillus niger, Cochliobolus heterostrophus and Neurospora crassa was also observed. Growth of Aspergillus flavus, Fusarium graminearum and Aspergillus oryzae was significantly inhibited (p < 0.001) at gliotoxin (10 lg/ml), indicating differential gliotoxin sensitivity amongst fungi. Re-introduction of gliT into A. fumigatus DgliT, at a different locus (ctsD; AFUA_4G07040, an aspartic protease), with selection on gliotoxin, facilitated deletion of ctsD without use of additional antibiotic selection markers. Absence of ctsD expression was accompanied by restoration of gliT expression, and resistance to gliotoxin. Thus, we propose gliT/gliotoxin as a useful selection marker system for fungal transformation. Finally, we suggest incorporation of gliotoxin sensitivity assays into all future fungal functional genomic studies