5 research outputs found
An assay for secologanin in plant tissues based on enzymatic conversion into strictosidine
The secoiridoid glucoside secologanin is the terpenoid building block in the biosynthesis of terpenoid indole
alkaloids. A method for its determination in plant tissues and in cell suspension cultures has been developed.
This assay is based on the condensation of secologanin with tryptamine, yielding strictosidine, in a reaction
catalysed by the enzyme strictosidine synthase (STR; E.C. 4.3.3.2). Subsequently, the formation of
strictosidine is quantified by high performance liquid chromatography (HPLC). STR was isolated from
transgenic Nicotiana tabacum cells expressing a cDNA-derived gene coding for STR from Catharanthus
roseus. The high specificity of STR for secologanin, in combination with a sensitive and selective HPLC
system, allows a simple extraction of secologanin from plant tissue. The detection limit of this method is
15 ng secologanin. Using this assay, secologanin contents were determined in tissues of various plant species;
Lonicera xylosteum hairy roots were found to contain 1% of secologanin on a dry weight basis. # 1998 John
Wiley & Sons, Ltd.info:eu-repo/semantics/publishedVersio
Influence of Precursor Availability on Alkaloid Accumulation by Transgenic Cell Line of Catharanthus roseus
An assay for secologanin in plant tissues based on enzymatic conversion into strictosidine
An assay for secologanin in plant tissues based on enzymatic conversion into strictosidine
The secoiridoid glucoside secologanin is the terpenoid building block in the biosynthesis of terpenoid indole
alkaloids. A method for its determination in plant tissues and in cell suspension cultures has been developed.
This assay is based on the condensation of secologanin with tryptamine, yielding strictosidine, in a reaction
catalysed by the enzyme strictosidine synthase (STR; E.C. 4.3.3.2). Subsequently, the formation of
strictosidine is quantified by high performance liquid chromatography (HPLC). STR was isolated from
transgenic Nicotiana tabacum cells expressing a cDNA-derived gene coding for STR from Catharanthus
roseus. The high specificity of STR for secologanin, in combination with a sensitive and selective HPLC
system, allows a simple extraction of secologanin from plant tissue. The detection limit of this method is
15 ng secologanin. Using this assay, secologanin contents were determined in tissues of various plant species;
Lonicera xylosteum hairy roots were found to contain 1% of secologanin on a dry weight basis. # 1998 John
Wiley & Sons, Ltd.info:eu-repo/semantics/publishedVersio