11 research outputs found
A New MAP Kinase Protein Involved in Estradiol-Stimulated Reproduction of the Helminth Parasite Taenia crassiceps
MAP kinases (MAPK) are involved in the regulation of cellular
processes such as reproduction and growth. In parasites, the role
of MAPK has been scarcely studied. Here, we describe the
participation of an ERK-like protein in estrogen-dependent
reproduction of the helminth parasite Taenia
crassiceps. Our results show that 17ÎČ-estradiol
induces a concentration-dependent increase in the bud number of in
vitro cultured cysticerci. If parasites are also incubated in
presence of an ERK-inhibitor, the stimulatory effect of estrogen
is blocked. The expression of ERK-like mRNA and its corresponding
protein was detected in the parasite. The ERK-like protein was
over-expressed by all treatments. Nevertheless, a strong induction
of phosphorylation of this protein was observed only in response
to 17ÎČ-estradiol. Cross-contamination by host cells was
discarded by flow cytometry analysis. Parasite cells expressing
the ERK-like protein were exclusively located at the subtegument
tissue by confocal microscopy. Finally, the ERK-like protein was
separated by bidimensional electrophoresis and then sequenced,
showing the conserved TEY activation motif, typical of all known
ERK 1/2 proteins. Our results show that an ERK-like protein is
involved in the molecular signalling during the interaction
between the host and T. crassiceps, and may be
considered as target for anti-helminth drugs design
La señalizaciĂłn a travĂ©s de los receptores FcyRI y FcyRII en cĂ©lulas monocĂticas es determinada por el estado de diferenciaciĂłn celular /
\ua0tesis que para obtener el grado de Doctor en Ciencias Biomédicas, presenta Claudia Hallal Calleros ; asesor Enrique Ortega Soto. 85, [11] påginas :\ua0ilustraciones. Doctorado en Ciencias Biomédicas\ua0UNAM, Instituto de Investigaciones Biomédicas,\ua0200
In Vitro Ovicidal and Cestocidal Effects of Toxins from Bacillus thuringiensis on the Canine and Human Parasite Dipylidium caninum
Bacillus thuringiensis is a gram-positive soil-dwelling bacterium that is commonly used as a biological pesticide. is bacterium may also be used for biological control of helminth parasites in domestic animals. In this study, we evaluated the possible ovicidal and cestocidal effects of a total protein extract of B. thuringiensis native strains on the zoonotic cestode parasite of dogs, Dipylidium caninum (D. caninum). Dose and time response curves were determined by coincubating B. thuringiensis proteins at concentration ranging from 100 to 1000 g/mL along with 4000 egg capsules of D. caninum. Egg viability was evaluated using the trypan blue exclusion test. e lethal concentration of toxins on eggs was 600 g/ml, and the best incubation time to produce this effect was 3 h. In the adult stage, the motility and the thickness of the tegument were used as indicators of damage. e motility was inhibited by 100% aer 8 hours of culture compared to the control group, while the thickness of the cestode was reduced by 34%. Conclusively, proteins of the strain GP526 of B. thuringiensis directly act upon D. caninum showing ovicidal and cestocidal effects. us, B. thuringiensis is proposed as a potential biological control agent against this zoonosis
In Vitro Ovicidal and Cestocidal Effects of Toxins from Bacillus thuringiensis on the Canine and Human Parasite Dipylidium caninum
Bacillus thuringiensis is a gram-positive soil-dwelling bacterium that is commonly used as a biological pesticide. This bacterium may also be used for biological control of helminth parasites in domestic animals. In this study, we evaluated the possible ovicidal and cestocidal effects of a total protein extract of B. thuringiensis native strains on the zoonotic cestode parasite of dogs, Dipylidium caninum (D. caninum). Dose and time response curves were determined by coincubating B. thuringiensis proteins at concentration ranging from 100 to 1000âÎŒg/mL along with 4000 egg capsules of D. caninum. Egg viability was evaluated using the trypan blue exclusion test. The lethal concentration of toxins on eggs was 600âÎŒg/ml, and the best incubation time to produce this effect was 3âh. In the adult stage, the motility and the thickness of the tegument were used as indicators of damage. The motility was inhibited by 100% after 8 hours of culture compared to the control group, while the thickness of the cestode was reduced by 34%. Conclusively, proteins of the strain GP526 of B. thuringiensis directly act upon D. caninum showing ovicidal and cestocidal effects. Thus, B. thuringiensis is proposed as a potential biological control agent against this zoonosis