3 research outputs found
Computational analysis of amino acids and their sidechain analogs in crowded solutions of RNA nucleobases with implications for the mRNA–protein complementarity hypothesis
Many critical processes in the cell involve direct binding between RNAs and proteins, making it imperative to fully understand the physicochemical principles behind such interactions at the atomistic level. Here, we use molecular dynamics simulations and 15 μs of sampling to study the behavior of amino acids and amino acid sidechain analogs in high-concentration aqueous solutions of standard RNA nucleobases. Structural and energetic analysis of simulated systems allows us to derive interaction propensity scales for different amino acid/nucleobase combinations. The derived scales closely match and greatly extend the available experimental data, providing a comprehensive foundation for studying RNA–protein interactions in different contexts. By using these scales, we demonstrate a statistically significant connection between nucleobase composition of human mRNA coding sequences and nucleobase interaction propensities of their cognate protein sequences. For example, pyrimidine density profiles of mRNAs match uracil-propensity profiles of their cognate proteins with a median Pearson correlation coefficient of R = −0.70. Our results provide support for the recently proposed hypotheses that mRNAs and their cognate proteins may be physicochemically complementary to each other and bind, especially if unstructured, with the complementarity level being negatively influenced by mRNA adenine content. Finally, we utilize the derived scales to refine the complementarity hypothesis and closely examine its physicochemical underpinnings.ISSN:1362-4962ISSN:0301-561
Inosine Nucleobase Acts as Guanine in Interactions with Protein Side Chains
A central intermediate
in purine catabolism, the inosine nucleobase
hypoxanthine is also one of the most abundant modified nucleobases
in RNA and plays key roles in the regulation of gene expression and
determination of cell fate. It is known that hypoxanthine acts as
guanine when interacting with other nucleobases and base pairs most
favorably with cytosine. However, its preferences when it comes to
interactions with amino acids remain unknown. Here we present for
the first time the absolute binding free energies and the associated
interaction modes between hypoxanthine and all standard, non-glycyl/non-prolyl
amino acid side chain analogs as derived from molecular dynamics simulations
and umbrella sampling in high- and low-dielectric environments. We
illustrate the biological relevance of the derived affinities by providing
a quantitative explanation for the specificity of hypoxanthine-guanine
phosphoribosyltransferase, a key enzyme in the purine salvage pathway.
Our results demonstrate that in its affinities for protein side chains,
hypoxanthine closely matches guanine, much more so than its precursor
adenine