1 research outputs found
Real-Time Cell-Electronic Sensing of Coal Fly Ash Particulate Matter for Toxicity-Based Air Quality Monitoring
The development of
a unique bioassay for cytotoxicity analysis
of coal fly ash (CFA) particulate matter (PM) and its potential application
for air quality monitoring is described. Using human cell lines, A549
and SK-MES-1, as live probes on microelectrode-embedded 96-well sensors,
impedance changes over time are measured as cells are treated with
varying concentrations (1 μg/mL–20 mg/mL) of CFA samples.
A dose-dependent impedance change is determined for each CFA sample,
from which an IC<sub>50</sub> histogram is obtained. The assay was
successfully applied to examine CFA samples collected from three coal-fired
power plants (CFPs) in China. The samples were separated into three
size fractions: PM2.5 (<2.5 μm), PM10-2.5 (2.5 μm < <i>x</i> < 10 μm), and PM10 (>10 μm). Dynamic
cell-response
profiles and temporal IC<sub>50</sub> histograms of all samples show
that CFA cytotoxicity depends on concentration, exposure time (0–60
h), and cell-type (SK-MES-1 > A549). The IC<sub>50</sub> values
differentiate
the cytotoxicity of CFA samples based on size fraction (PM2.5 ≈
PM10-2.5 ≫ PM10) and the sampling location (CFP2 > CFP1
≈
CFP3). Differential cytotoxicity measurements of particulates in human
cell lines using cell-electronic sensing provide a useful tool for
toxicity-based air quality monitoring and risk assessment