Simultaneous Determination of Co-formulated Matrine and Secnidazole in Suppositories by Reverse Phase High Performance Liquid Chromatography

Purpose: To develop and validate a new, simple, sensitive and accurate high performance liquid chromatographic (HPLC) method for the simulataneous determination of matrine and secnidazole in suppositories.Methods: The method involved using a SinoChoom ODS-BP C18 column (5 ƒÊm, 4.6 mm ~ 200 mm) and mobile phase consisting of  acetonitrile.triethylamine (0.05 %) in 0.025 mol/L KH2PO4 (20:80, v/v).The flow rate was 1 mL/min and detection was monitored at 210 and 311 nm for matrine and secnidazole, respectively. Total run time was 10 min and the column was maintained at 35 oC.Results: The excipients in the suppository did not interfere with the drug peaks. Matrine was eluted at a retention time (RT) of 4.30 min while linearity for the quantification of drug was obtained in the concentration range of 10.0 - 100.0 ƒÊg/mL (r2 = 0.9991). Secnidazole was eluted at a retention time (tr) of 6.69 min and linearity for the quantification of the drug was obtained in the concentration range of 10.0 - 150.0 ƒÊg/mL (r2 = 0.9993). Intra- and inter-day variations were < 1.0 % for both matrine andsecnidazole.Conclusion: The developed HPLC method was validated according to International Conference on Harmonisation (ICH) guidelines and proved to be suitable for the simultaneous determination of matrine and secnidazole in suppositories.Keywords: Matrine, Secnidazole, Suppository, HPLC, Assa

2-(Ethyl­sulfin­yl)imidazo[1,2-a]pyridine-3-sulfonamide

The supra­molecular structure of the title compound, C9H11N3O3S2, is defined by two inter­molecular hydrogen bonds. Pairs of N—H⋯N hydrogen bonds link the mol­ecules into centrosymmetric dimers and N—H⋯O hydrogen bonds link the dimers into a tubular chain structure running parallel to the a axis

Cooperative Beamforming Design for Multiple RIS-Assisted Communication Systems

Reconfigurable intelligent surface (RIS) provides a promising way to build programmable wireless transmission environments. Owing to the massive number of controllable reflecting elements on the surface, RIS is capable of providing considerable passive beamforming gains. At present, most related works mainly consider the modeling, design, performance analysis and optimization of single-RIS-assisted systems. Although there are a few of works that investigate multiple RISs individually serving their associated users, the cooperation among multiple RISs is not well considered as yet. To fill the gap, this paper studies a cooperative beamforming design for multi-RIS-assisted communication systems, where multiple RISs are deployed to assist the downlink communications from a base station to its users. To do so, we first model the general channel from the base station to the users for arbitrary number of reflection links. Then, we formulate an optimization problem to maximize the sum rate of all users. Analysis shows that the formulated problem is difficult to solve due to its non-convexity and the interactions among the decision variables. To solve it effectively, we first decouple the problem into three disjoint subproblems. Then, by introducing appropriate auxiliary variables, we derive the closed-form expressions for the decision variables and propose a low-complexity cooperative beamforming algorithm. Simulation results have verified the effectiveness of the proposed algorithm through comparison with various baseline methods. Furthermore, these results also unveil that, for the sum rate maximization, distributing the reflecting elements among multiple RISs is superior to deploying them at one single RIS

Loureirin B attenuates amiodarone-induced pulmonary fibrosis by suppression of TGFβ1/Smad2/3 pathway

Purpose: To investigate the therapeutic effect of loureirin B (LB) on amiodarone (AD)-induced pulmonary fibrosis (PF).Methods: Forty-eight male C57BL/6 mice, 8–10 weeks of age, were divided into four groups (n=12). Oral administration of amiodarone hydrochloride (AD) was performed for 4 weeks to induce pulmonary fibrosis. The degree of fibrosis was assessed by Masson staining, while collagen I and α-smooth muscle actin (α-SMA) levels were evaluated by Western blot analysis. ELISA was used to measure the levels of cytokines TNF-α, IL-1β, and IL-6 in bronchoalveolar lavage fluid (BALF) and lung tissue. Levels of p- Smad2, Smad2, p-Smad3 and Smad3 were determined by western blotting.Results: AD treatment increased the collagen levels and expression levels of collagen I and α-smooth muscle actin (α-SMA) in lung tissue and of inflammatory cytokines TNF-α, IL-1β, and IL-6, in both bronchoalveolar lavage fluid (BALF) and lung tissue in a dose-dependent manner (p < 0.01).Furthermore, AD increased the levels of p-Smad2/3. AD-induced increases in collagen I and α-SMA levels were reversed by loureirin B (LB). In addition, LB reduced AD-induced increased levels of the inflammatory cytokines TNF-α, IL-1β, and IL-6 in both bronchoalveolar lavage fluid (BALF) and lung tissue (p < 0.01).Conclusion: These results demonstrate that LB downregulates expression of fibrosis-related proteins and suppresses AD-induced PF. The  mechanism responsible for the protective effect of LB on ADinduced PF might involve inhibition of the Smad2/3 pathway. Thus, LB is a potential therapeutic agent for the management of PF. Keywords: Amiodarone, Loureirin B, Pulmonary fibrosis, Smad, Inflammatio

Combustion Catalyst: Nano‐Fe2O3 and Nano‐Thermite Al/ Fe2O3 with Different Shapes

In order to enable the energetic materials to possess a more powerful performance, adding combustion catalysts is a quite effective method. Granular, oval, and polyhedral Fe2O3 particles have been prepared by the hydrothermal method and used to fabricate Al/Fe2O3 thermites. All the Fe2O3 and Al/Fe2O3 thermite samples were characterized using a combination of experimental techniques including scanning electron microscopy (SEM), energy dispersive spectrometer (EDS), X‐ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), transmission electron microscope (TEM), and high‐resolution TEM (HRTEM). The non‐isothermal decomposition kinetics of the composites and nitrocellulose (NC) can be modeled by the Avrami‐Erofeev equation f(α)=3(1–α)[–ln(1–α)]1/3/2 in differential form. Through the thermogravimetric analysis infrared (TG‐IR) analysis of decomposition processes and products, it is speculated that Fe2O3 and Al/Fe2O3 can effectively accelerate the thermal decomposition reaction rate of NC by promoting the O‐NO2 bond cleavage. Adding oxides or thermites can distinctly increase the burning rate, decrease the burning rate pressure exponent, increase the flame temperature, and improve the combustion wave structures of the ammonium perchlorate/hydroxyl‐terminated polybutadiene (AP/HTPB) propellants. Among the three studied, different shapes of Fe2O3, the granular Fe2O3, and its corresponding thermites (Al/Fe2O3(H)) exhibit the highest burning rate due to larger surface area associated with smaller particle size. Moreover, Al/Fe2O3(H) thermites have more effective combustion‐supporting ability for AP/HTPB propellants than Fe2O3 structures and the other two as‐prepared Al/Fe2O3 thermites

A Blockchain Traceable Scheme with Oversight Function

Many blockchain researches focus on the privacy protection. However, criminals can leverage strong privacy protection of the blockchain to do illegal crimes (such as ransomware) without being punished. These crimes have caused huge losses to society and users. Implementing identity tracing is an important step in dealing with issues arising from privacy protection. In this paper, we propose a blockchain traceable scheme with oversight function (BTSOF). The design of BTSOF builds on SkyEye (Tianjun Ma et al., Cryptology ePrint Archive 2020). In BTSOF, the regulator must obtain the consent of the committee to enable tracing. Moreover, we construct a non-interactive verifiable multi-secret sharing scheme (VMSS scheme) and leverage the VMSS scheme to design a distributed multi-key generation (DMKG) protocol for the Cramer-Shoup public key encryption scheme. The DMKG protocol is used in the design of BTSOF. We provide the security definition and security proof of the VMSS scheme and DMKG protocol

SkyEye: A Traceable Scheme for Blockchain

Many studies focus on the blockchain privacy protection. Unfortunately, the privacy protection brings some issues (e.g., money-laundering problem). Tracing users\u27 identities is a critical step in addressing these issues. When each user\u27s identity in the blockchain data is determined, the regulator can do some regulatory operations (such as Big Data analysis) to decide who should be punished or who should own the lost data. In this paper, we propose SkyEye, a traceable scheme for blockchain, that can be applied to a class of blockchain application. SkyEye enables the regulator to trace users\u27 identities. Moreover, we demonstrate the security of SkyEye under specific cryptographic assumptions. Finally, we implement two prototypes of SkyEye, and evaluate the running time and related data storage requirements by performing the aforementioned prototypes

Proteins Identified from Saliva and Salivary Glands of the Chinese Gall Aphid Schlechtendalia chinensis

Aphid saliva plays an essential role in the interaction between aphids and their host plants. Several aphid salivary proteins have been identified but none from galling aphids. Here the salivary proteins from the Chinese gall aphid are analyzed, Schlechtendalia chinensis, via an LC-MS/MS analysis. A total of 31 proteins are identified directly from saliva collected via an artificial diet, and 141 proteins are identified from extracts derived from dissected salivary glands. Among these identified proteins, 17 are found in both collected saliva and dissected salivary glands. In comparison with salivary proteins from ten other free-living Hemipterans, the most striking feature of the salivary protein from S. chinensis is the existence of high proportion of proteins with binding activity, including DNA-, protein-, ATP-, and iron-binding proteins. These proteins maybe involved in gall formation. These results provide a framework for future research to elucidate the molecular basis for gall induction by galling aphids

Comprehensive transcriptome analysis reveals novel genes involved in cardiac glycoside biosynthesis and mlncRNAs associated with secondary metabolism and stress response in Digitalis purpurea

<p>Abstract</p> <p>Background</p> <p><it>Digitalis purpurea </it>is an important ornamental and medicinal plant. There is considerable interest in exploring its transcriptome.</p> <p>Results</p> <p>Through high-throughput 454 sequencing and subsequent assembly, we obtained 23532 genes, of which 15626 encode conserved proteins. We determined 140 unigenes to be candidates involved in cardiac glycoside biosynthesis. It could be grouped into 30 families, of which 29 were identified for the first time in <it>D. purpurea</it>. We identified 2660 mRNA-like npcRNA (mlncRNA) candidates, an emerging class of regulators, using a computational mlncRNA identification pipeline and 13 microRNA-producing unigenes based on sequence conservation and hairpin structure-forming capability. Twenty five protein-coding unigenes were predicted to be targets of these microRNAs. Among the mlncRNA candidates, only 320 could be grouped into 140 families with at least two members in a family. The majority of <it>D. purpurea </it>mlncRNAs were species-specific and many of them showed tissue-specific expression and responded to cold and dehydration stresses. We identified 417 protein-coding genes with regions significantly homologous or complementary to 375 mlncRNAs. It includes five genes involved in secondary metabolism. A positive correlation was found in gene expression between protein-coding genes and the homologous mlncRNAs in response to cold and dehydration stresses, while the correlation was negative when protein-coding genes and mlncRNAs were complementary to each other.</p> <p>Conclusions</p> <p>Through comprehensive transcriptome analysis, we not only identified 29 novel gene families potentially involved in the biosynthesis of cardiac glycosides but also characterized a large number of mlncRNAs. Our results suggest the importance of mlncRNAs in secondary metabolism and stress response in <it>D. purpurea</it>.</p