94 research outputs found

    The Combination of Human Urinary Kallidinogenase and Mild Hypothermia Protects Adult Rats Against Hypoxic-Ischemic Encephalopathy-Induced Injury by Promoting Angiogenesis and Regeneration

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    Objectives: Human Urinary Kallidinogenase (HUK) is a tissue kallikrein that plays neuroprotective role in ischemic conditions via different mechanisms. Mild hypothermia (MH) is another robust neuroprotectant that reduces mortality but does not profoundly ameliorate the neurological outcome in hypoxic-ischemic encephalopathy (HIE) patients. However, whether the combination of HUK and MH can be used as a promising neuroprotective treatment in HIE is unknown. Methods: One-hundred and forty-four adult Wistar rats were randomly divided into five groups: Sham, HIE, HUK, MH and a combination of HUK and MH treatment. The HIE rat model was established by right carotid dissection followed by hypoxia aspiration. The survival curve was created within 7 days, and the neurological severity scores (NSS) were assessed at days 0, 1, 3, and 7. Nissl staining, Terminal deoxynucleotidyl transferase-mediated dUTP nick end-labeling (TUNEL), immunofluorescent staining and western blotting were used to evaluate neuronal survival, apoptosis and necrosis, tight-junction proteins Claudin-1 and Zonula occludens-1 (ZO-1), vascular endothelial growth factor (VEGF), doublecortex (DCX), bradykinin receptor B1 (BDKRB1), BDKRB2 and Ki67 staining. Results: The combined treatment rescued all HIE rats from death and had a best survival curve compared to HIE. The Combination also reduced the NSS scores after HIE at days 7, better than HUK or MH alone. The combination of HUK and MH reserved more cells in Nissl staining and inhibited neuronal apoptosis and necrosis as well as significantly attenuated HIE-induced decreases in claudin-1, ZO-1, cyclin D1 and BDKRB1/B2 in comparison to HUK or MH treatment alone. Moreover, the combined treatment increased the expression of VEGF and DCX as well as the number of Ki67-labeled cells. Conclusions: This study demonstrates that both HUK and MH are neuroprotective after HIE insult; however, the combined therapy with HUK and MH enhanced the efficiency and efficacy of either therapy alone in the treatment of HIE, at least partially by promoting angiogenesis and regeneration and rescuing tight-junction loss. The combination of HUK and MH seems to be a feasible and promising clinical strategy to alleviate cerebral injury following HIE insult. Highlights: -The combination of HUK and MH distinctly reduces neurological dysfunction in HIE rats.-HUK enhances the neuroprotective effects of MH in HIE.-MH attenuates tight-junction disruption, upregulates the BDKR B1/2, DCX and cyclin D1.-The combination of MH and HUK enhances the expressions of MH/HUK mediated-BDKR B1/2, DCX, cyclin D1 and Ki67 positive cells

    The Combination of Human Urinary Kallidinogenase and Mild Hypothermia Protects Adult Rats Against Hypoxic-Ischemic Encephalopathy-Induced Injury by Promoting Angiogenesis and Regeneration

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    Objectives: Human Urinary Kallidinogenase (HUK) is a tissue kallikrein that plays neuroprotective role in ischemic conditions via different mechanisms. Mild hypothermia (MH) is another robust neuroprotectant that reduces mortality but does not profoundly ameliorate the neurological outcome in hypoxic-ischemic encephalopathy (HIE) patients. However, whether the combination of HUK and MH can be used as a promising neuroprotective treatment in HIE is unknown.Methods: One-hundred and forty-four adult Wistar rats were randomly divided into five groups: Sham, HIE, HUK, MH and a combination of HUK and MH treatment. The HIE rat model was established by right carotid dissection followed by hypoxia aspiration. The survival curve was created within 7 days, and the neurological severity scores (NSS) were assessed at days 0, 1, 3, and 7. Nissl staining, Terminal deoxynucleotidyl transferase-mediated dUTP nick end-labeling (TUNEL), immunofluorescent staining and western blotting were used to evaluate neuronal survival, apoptosis and necrosis, tight-junction proteins Claudin-1 and Zonula occludens-1 (ZO-1), vascular endothelial growth factor (VEGF), doublecortex (DCX), bradykinin receptor B1 (BDKRB1), BDKRB2 and Ki67 staining.Results: The combined treatment rescued all HIE rats from death and had a best survival curve compared to HIE. The Combination also reduced the NSS scores after HIE at days 7, better than HUK or MH alone. The combination of HUK and MH reserved more cells in Nissl staining and inhibited neuronal apoptosis and necrosis as well as significantly attenuated HIE-induced decreases in claudin-1, ZO-1, cyclin D1 and BDKRB1/B2 in comparison to HUK or MH treatment alone. Moreover, the combined treatment increased the expression of VEGF and DCX as well as the number of Ki67-labeled cells.Conclusions: This study demonstrates that both HUK and MH are neuroprotective after HIE insult; however, the combined therapy with HUK and MH enhanced the efficiency and efficacy of either therapy alone in the treatment of HIE, at least partially by promoting angiogenesis and regeneration and rescuing tight-junction loss. The combination of HUK and MH seems to be a feasible and promising clinical strategy to alleviate cerebral injury following HIE insult.Highlights: -The combination of HUK and MH distinctly reduces neurological dysfunction in HIE rats.-HUK enhances the neuroprotective effects of MH in HIE.-MH attenuates tight-junction disruption, upregulates the BDKR B1/2, DCX and cyclin D1.-The combination of MH and HUK enhances the expressions of MH/HUK mediated-BDKR B1/2, DCX, cyclin D1 and Ki67 positive cells

    Synthesis and White-Light Emission of ZnO/HfO2: Eu Nanocables

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    ZnO/HfO2:Eu nanocables were prepared by radio frequency sputtering with electrospun ZnO nanofibers as cores. The well-crystallized ZnO/HfO2:Eu nanocables showed a uniform intact core–shell structure, which consisted of a hexagonal ZnO core and a monoclinic HfO2 shell. The photoluminescence properties of the samples were characterized. A white-light band emission consisted of blue, green, and red emissions was observed in the nanocables. The blue and green emissions can be attributed to the zinc vacancy and oxygen vacancy defects in ZnO/HfO2:Eu nanocables, and the yellow–red emissions are derived from the inner 4f-shell transitions of corresponding Eu3+ ions in HfO2:Eu shells. Enhanced white-light emission was observed in the nanocables. The enhancement of the emission is ascribed to the structural changes after coaxial synthesis

    A study of CP violation in B-+/- -> DK +/- and B-+/- -> D pi(+/-) decays with D -> (KSK +/-)-K-0 pi(-/+) final states

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    A first study of CP violation in the decay modes B±→[KS0K±π∓]Dh±B^\pm\to [K^0_{\rm S} K^\pm \pi^\mp]_D h^\pm and B±→[KS0K∓π±]Dh±B^\pm\to [K^0_{\rm S} K^\mp \pi^\pm]_D h^\pm, where hh labels a KK or π\pi meson and DD labels a D0D^0 or D‾0\overline{D}^0 meson, is performed. The analysis uses the LHCb data set collected in pppp collisions, corresponding to an integrated luminosity of 3 fb−1^{-1}. The analysis is sensitive to the CP-violating CKM phase γ\gamma through seven observables: one charge asymmetry in each of the four modes and three ratios of the charge-integrated yields. The results are consistent with measurements of γ\gamma using other decay modes

    Studies of beauty baryon decays to D0ph− and Λ+ch− final states

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    Search for CP violation using T-odd correlations in D-0 -> K+K-pi(+)pi(-) decays

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    A search for CPCP violation using TT-odd correlations is performed using the four-body D0→K+K−π+π−D^0 \to K^+K^-\pi^+\pi^- decay, selected from semileptonic BB decays. The data sample corresponds to integrated luminosities of 1.0 fb−11.0\,\text{fb}^{-1} and 2.0 fb−12.0\,\text{fb}^{-1} recorded at the centre-of-mass energies of 7 TeV and 8 TeV, respectively. The CPCP-violating asymmetry aCPT-odda_{CP}^{T\text{-odd}} is measured to be (0.18±0.29(stat)±0.04(syst))%(0.18\pm 0.29\text{(stat)}\pm 0.04\text{(syst)})\%. Searches for CPCP violation in different regions of phase space of the four-body decay, and as a function of the D0D^0 decay time, are also presented. No significant deviation from the CPCP conservation hypothesis is found

    Measurement of CP asymmetry in B-s(0) -> D-s(-/+) K--/+ decays

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    We report on measurements of the time-dependent CP violating observables in Bs0→Ds∓K±B^0_s\rightarrow D^{\mp}_s K^{\pm} decays using a dataset corresponding to 1.0 fb−1^{-1} of pp collisions recorded with the LHCb detector. We find the CP violating observables Cf=0.53±0.25±0.04C_f=0.53\pm0.25\pm0.04, AfΔΓ=0.37±0.42±0.20A^{\Delta\Gamma}_f=0.37\pm0.42\pm0.20, AfˉΔΓ=0.20±0.41±0.20A^{\Delta\Gamma}_{\bar{f}}=0.20\pm0.41\pm0.20, Sf=−1.09±0.33±0.08S_f=-1.09\pm0.33\pm0.08, Sfˉ=−0.36±0.34±0.08S_{\bar{f}}=-0.36\pm0.34\pm0.08, where the uncertainties are statistical and systematic, respectively. We use these observables to make the first measurement of the CKM angle γ\gamma in Bs0→Ds∓K±B^0_s\rightarrow D^{\mp}_s K^{\pm} decays, finding γ\gamma = (115−43+28_{-43}^{+28})∘^\circ modulo 180∘^\circ at 68% CL, where the error contains both statistical and systematic uncertainties.We report on measurements of the time-dependent CP violating observables in Bs0_{s}^{0}  → Ds∓_{s}^{∓} K±^{±} decays using a dataset corresponding to 1.0 fb−1^{−1} of pp collisions recorded with the LHCb detector. We find the CP violating observables Cf_{f} = 0.53±0.25±0.04, AfΔΓ_{f}^{ΔΓ}  = 0.37 ± 0.42 ± 0.20, Af‾ΔΓ=0.20±0.41±0.20 {A}_{\overline{f}}^{\varDelta \varGamma }=0.20\pm 0.41\pm 0.20 , Sf_{f} = −1.09±0.33±0.08, Sf‾=−0.36±0.34±0.08 {S}_{\overline{f}}=-0.36\pm 0.34\pm 0.08 , where the uncertainties are statistical and systematic, respectively. Using these observables together with a recent measurement of the Bs0_{s}^{0} mixing phase −2βs_{s} leads to the first extraction of the CKM angle γ from Bs0_{s}^{0}  → Ds∓_{s}^{∓} K±^{±} decays, finding γ = (115− 43+ 28_{− 43}^{+ 28} )° modulo 180° at 68% CL, where the error contains both statistical and systematic uncertainties.We report on measurements of the time-dependent CP violating observables in Bs0→Ds∓K±B^0_s\rightarrow D^{\mp}_s K^{\pm} decays using a dataset corresponding to 1.0 fb−1^{-1} of pp collisions recorded with the LHCb detector. We find the CP violating observables Cf=0.53±0.25±0.04C_f=0.53\pm0.25\pm0.04, AfΔΓ=0.37±0.42±0.20A^{\Delta\Gamma}_f=0.37\pm0.42\pm0.20, AfˉΔΓ=0.20±0.41±0.20A^{\Delta\Gamma}_{\bar{f}}=0.20\pm0.41\pm0.20, Sf=−1.09±0.33±0.08S_f=-1.09\pm0.33\pm0.08, Sfˉ=−0.36±0.34±0.08S_{\bar{f}}=-0.36\pm0.34\pm0.08, where the uncertainties are statistical and systematic, respectively. Using these observables together with a recent measurement of the Bs0B^0_s mixing phase −2βs-2\beta_s leads to the first extraction of the CKM angle γ\gamma from Bs0→Ds∓K±B^0_s \rightarrow D^{\mp}_s K^{\pm} decays, finding γ\gamma = (115−43+28_{-43}^{+28})∘^\circ modulo 180∘^\circ at 68% CL, where the error contains both statistical and systematic uncertainties

    Study of forward Z + jet production in pp collisions at √s=7 TeV

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    A measurement of the Z(→μ+μ−)Z(\rightarrow\mu^+\mu^-)+jet production cross-section in pppp collisions at a centre-of-mass energy s=7\sqrt{s} = 7 TeV is presented. The analysis is based on an integrated luminosity of 1.0 fb−11.0\,\text{fb}^{-1} recorded by the LHCb experiment. Results are shown with two jet transverse momentum thresholds, 10 and 20 GeV, for both the overall cross-section within the fiducial volume, and for six differential cross-section measurements. The fiducial volume requires that both the jet and the muons from the Z boson decay are produced in the forward direction (2.0<η<4.52.0<\eta<4.5). The results show good agreement with theoretical predictions at the second-order expansion in the coupling of the strong interaction.A measurement of the Z(→μ+μ−)Z(\rightarrow\mu^+\mu^-)+jet production cross-section in pppp collisions at a centre-of-mass energy s=7\sqrt{s} = 7 TeV is presented. The analysis is based on an integrated luminosity of 1.0 fb−11.0\,\text{fb}^{-1} recorded by the LHCb experiment. Results are shown with two jet transverse momentum thresholds, 10 and 20 GeV, for both the overall cross-section within the fiducial volume, and for six differential cross-section measurements. The fiducial volume requires that both the jet and the muons from the Z boson decay are produced in the forward direction (2.0<η<4.52.0<\eta<4.5). The results show good agreement with theoretical predictions at the second-order expansion in the coupling of the strong interaction

    Measurement of Upsilon production in collisions at root s=2.76 TeV

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    The production of Υ(1S)\Upsilon(1S), Υ(2S)\Upsilon(2S) and Υ(3S)\Upsilon(3S) mesons decaying into the dimuon final state is studied with the LHCb detector using a data sample corresponding to an integrated luminosity of 3.3 pb−1pb^{-1} collected in proton-proton collisions at a centre-of-mass energy of s=2.76\sqrt{s}=2.76 TeV. The differential production cross-sections times dimuon branching fractions are measured as functions of the Υ\Upsilon transverse momentum and rapidity, over the ranges $p_{\rm T} Upsilon(1S) X) x B(Upsilon(1S) -> mu+mu-) = 1.111 +/- 0.043 +/- 0.044 nb, sigma(pp -> Upsilon(2S) X) x B(Upsilon(2S) -> mu+mu-) = 0.264 +/- 0.023 +/- 0.011 nb, sigma(pp -> Upsilon(3S) X) x B(Upsilon(3S) -> mu+mu-) = 0.159 +/- 0.020 +/- 0.007 nb, where the first uncertainty is statistical and the second systematic
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