Poly I:C enhances cycloheximide-induced apoptosis of tumor cells through TLR3 pathway-3

<p><b>Copyright information:</b></p><p>Taken from "Poly I:C enhances cycloheximide-induced apoptosis of tumor cells through TLR3 pathway"</p><p>http://www.biomedcentral.com/1471-2407/8/12</p><p>BMC Cancer 2008;8():12-12.</p><p>Published online 17 Jan 2008</p><p>PMCID:PMC2242792.</p><p></p>mouse TLR3 antibody at the concentration of 10 μg/ml for 4 hours before being stimulated with poly I:C (100 μg/ml)/CHX (1.5 μg/ml) for 12 hours. Apoptosis were determined as above. The data is presented as the mean ± SD

Poly I:C enhances cycloheximide-induced apoptosis of tumor cells through TLR3 pathway-5

<p><b>Copyright information:</b></p><p>Taken from "Poly I:C enhances cycloheximide-induced apoptosis of tumor cells through TLR3 pathway"</p><p>http://www.biomedcentral.com/1471-2407/8/12</p><p>BMC Cancer 2008;8():12-12.</p><p>Published online 17 Jan 2008</p><p>PMCID:PMC2242792.</p><p></p>RT-PCR. One representative experiment (out of three) is depicted. (B, C). FACS analysis of intracellular(B) and cell surface(C) expression of TLR3 in tumor cell lines. Histograms showed TLR3 expression (shadow area) with isotype controls (black lines)

Poly I:C enhances cycloheximide-induced apoptosis of tumor cells through TLR3 pathway-1

<p><b>Copyright information:</b></p><p>Taken from "Poly I:C enhances cycloheximide-induced apoptosis of tumor cells through TLR3 pathway"</p><p>http://www.biomedcentral.com/1471-2407/8/12</p><p>BMC Cancer 2008;8():12-12.</p><p>Published online 17 Jan 2008</p><p>PMCID:PMC2242792.</p><p></p>poly I:C plus CHX for 72 h. 1×PBS was added as control. 40 μl of CellTiter 96AQueous One Solution were added to each well and incubated for additional 3 hrs before final examination with absorbance at 490 nm. Optical density value shows the viability of cells tested. Results are from one representative experiment of three repeated experiments

Poly I:C enhances cycloheximide-induced apoptosis of tumor cells through TLR3 pathway-0

<p><b>Copyright information:</b></p><p>Taken from "Poly I:C enhances cycloheximide-induced apoptosis of tumor cells through TLR3 pathway"</p><p>http://www.biomedcentral.com/1471-2407/8/12</p><p>BMC Cancer 2008;8():12-12.</p><p>Published online 17 Jan 2008</p><p>PMCID:PMC2242792.</p><p></p>RT-PCR. One representative experiment (out of three) is depicted. (B, C). FACS analysis of intracellular(B) and cell surface(C) expression of TLR3 in tumor cell lines. Histograms showed TLR3 expression (shadow area) with isotype controls (black lines)

Therapeutic effects of STAT3 decoy oligodeoxynucleotide on human lung cancer in xenograft mice-1

<p><b>Copyright information:</b></p><p>Taken from "Therapeutic effects of STAT3 decoy oligodeoxynucleotide on human lung cancer in xenograft mice"</p><p>http://www.biomedcentral.com/1471-2407/7/149</p><p>BMC Cancer 2007;7():149-149.</p><p>Published online 4 Aug 2007</p><p>PMCID:PMC1988829.</p><p></p> cells were treated with 25 nmol/L STAT3 decoy ODN, STAT3 scramble ODN or vehicle control (TE) for different time using lipofectamine 2000. The cumulative number of cells was accounted using trypan blue exclusion. Values are expressed as mean ± SD of three independent experiments. A549 cells (4 × 10/well) were treated with STAT3 decoy ODN or scramble decoy ODN for 18 hours at 37°C, and then pulsed with [H]-thymidine for an additional 6 hours. The incorporation of [H]-thymidine was analyzed by liquid scintillation counting. A549 cells were plated in six well plate, after transfected with 25 nmol/L STAT3 decoy ODN or scramble ODN, the apoptotic A549 cells were detected by flow cytometry using Annexin V-FITC and PI. The histogram represented the percentage of apoptotic cells. Values are expressed as the mean ± SD of three independent experiments. Statistical significance was determined as *< 0.01 compared to other groups

Diagnostic utility of mRNA in peripheral blood and pleural fluid in patients with primary non-small cell lung cancer-1

Reference to the standard curve, as described in methods. The copy number of each mRNA was further normalized as the ratio to the copy number of in all samples. For each gene marker, when the copy number was less than 100, it could not be detectable as a negative case. All the negative results of each indicated gene were shown as number undetected. The median is marked as "" in each group. Where the frequency of negative cases is > 50%, the median cannot be shown.<p><b>Copyright information:</b></p><p>Taken from "Diagnostic utility of mRNA in peripheral blood and pleural fluid in patients with primary non-small cell lung cancer"</p><p>http://www.biomedcentral.com/1471-2407/8/156</p><p>BMC Cancer 2008;8():156-156.</p><p>Published online 31 May 2008</p><p>PMCID:PMC2424066.</p><p></p

Survival of liver MNC-derived cells in lethally irradiated mice.

<p>(A) Hepatic MNCs (10⁶) from CD45.1⁺ mice were mixed with 10⁶ support BM cells from CD45.2⁺ mice and transferred into lethally irradiated CD45.2⁺ B6 mice. (B–C) Two months post-transfer, CD45.1⁺ cells in the recipient liver, spleen, and BM were gated, and the expression of CD3 and CD19 was analyzed (n = 3 mice/group). (D) Hepatic MNCs (10⁶) from CD45.1⁺ mice were transferred into CD45.1⁻<i>Rag-1^−/−Il2rg^−/−</i> mice. (E) Two months post-transfer, CD45.1⁺ donor cells in the recipient liver, spleen, and BM were gated, and the expression of CD3e and CD19 were analyzed (n = 3 mice/group). All data are representative of 2 independent experiments.</p

Therapeutic effects of STAT3 decoy oligodeoxynucleotide on human lung cancer in xenograft mice-3

<p><b>Copyright information:</b></p><p>Taken from "Therapeutic effects of STAT3 decoy oligodeoxynucleotide on human lung cancer in xenograft mice"</p><p>http://www.biomedcentral.com/1471-2407/7/149</p><p>BMC Cancer 2007;7():149-149.</p><p>Published online 4 Aug 2007</p><p>PMCID:PMC1988829.</p><p></p> were harvested and the total cellular RNA was isolated using Trizol Reagent, mRNA levels of STAT3-correlated genes such as mcl-1, cyclin D1, bcl-xl, cyclinE, c-myc and survivin were detected using RT-PCR method. The PCR products were electrophoresed and photographed using AlphaEaseFC. After transfected with 25 nmol/L decoy ODN, the whole A549 were harvested and the whole-cell extracts were obtained. And western blotting for bcl-x1 and cyclin D1 were then examined as described in materials and methods. Histogram presented the relative expression level of each gene or protein after normalization to its corresponding internal control. Statistical significance was determined as *< 0.01 and < 0.05 compared to control

Characterizing the Lymphopoietic Kinetics and Features of Hematopoietic Progenitors Contained in the Adult Murine Liver In Vivo

<div><p>The appearance of donor-derived lymphocytes in liver transplant patients suggests that adult livers may contain cells capable of lymphopoiesis. However, only a few published studies have addressed the lymphopoietic capacity of adult liver cells, and its kinetics and features remain unclear. Herein, we investigated the lymphopoietic capacity of adult liver mononuclear cells (MNCs) and purified liver hematopoietic progenitor cells (HPCs) in vivo. Similar to bone-marrow transplantation (BMT), transplantation of liver MNCs alone was able to rescue survival of lethally irradiated mice. In terms of kinetics, liver MNC-derived myeloid lineage cells reconstituted more slowly than those from BMT. Liver MNC-derived lymphocyte lineage cells in the blood, spleen and BM also reconstituted more slowly than BMT, but lymphocytes in the liver recovered at a similar rate. Interestingly, liver MNCs predominantly gave rise to CD3⁺CD19⁻ T cells in both irradiated WT and non-irradiated lymphocyte-deficient <i>Rag-1^−/−Il2rg^−/−</i> recipients. To define the lymphopoietic potential of various cell populations within liver MNCs, we transplanted purified lineage-negative (Lin⁻) liver HPCs into recipient mice. Unlike total liver MNCs, liver HPCs reconstituted T and B cells in similar frequencies to BMT. We further determined that the predominance of T cells observed after transplanting total liver MNCs likely originated from mature T cells, as purified donor liver T cells proliferated in the recipients and gave rise to CD8⁺ T cells. Thus, the capacity of donor adult liver cells to reconstitute lymphocytes in recipients derives from both HPCs and mature T cells contained in the liver MNC population.</p></div

Therapeutic effects of STAT3 decoy oligodeoxynucleotide on human lung cancer in xenograft mice-4

<p><b>Copyright information:</b></p><p>Taken from "Therapeutic effects of STAT3 decoy oligodeoxynucleotide on human lung cancer in xenograft mice"</p><p>http://www.biomedcentral.com/1471-2407/7/149</p><p>BMC Cancer 2007;7():149-149.</p><p>Published online 4 Aug 2007</p><p>PMCID:PMC1988829.</p><p></p>immunohistochemical analysis of bcl-xl () and cyclin D1 () as described in materials and methods. The brown showed the expression levels of bcl-xl and cycln D1; the blue showed the nuclei (original magnification ×400). Each histogram presented the number of bcl-xl and cyclin D1 positive cells per High Power Field in tumor tissues. Statistical significance was determined as < 0.05