Additional file 1 of Causal associations between leisure sedentary behaviors and sleep status with frailty: insight from Mendelian randomization study

Supplementary Material

BCL11A mRNA expression in peripheral blood from pediatric β-thal patients.

(A) qRT-PCR analysis revealed that the expression levels of BCL11A mRNA were decreased in pediatric β-thal patients compared to healthy controls. (B) The dynamic of hsa-miR-190b-5p expression levels at different ages of healthy controls and pediatric β-thal patients. (C) Pearson’s correlation test analysis of the correlation between hsa-miR-190b-5p level and BCL11A mRNA expression in pediatric β-thal patients. BCL11A: B-cell lymphoma/leukemia 11A. Data expressed as mean ± SD. Compared with healthy controls, *pnsp>0.05.</p

Western blot analysis of the protein expression levels of BCL11A in 293T cells after transfected hsa-miR-190b-5p mimics and inhibitors.

Western blot analysis of the protein expression levels of BCL11A in 293T cells after transfected hsa-miR-190b-5p mimics and inhibitors.</p

hsa-miR-190b-5p expression in peripheral blood from pediatric β-thal patients.

(A) qRT-PCR analysis showed that hsa-miR-190b-5p expression levels were upregulated in pediatric β-thal patients in comparison with healthy controls. (B) The dynamic of hsa-miR-190b-5p expression levels at different ages of healthy controls and pediatric β-thal patients. miR: microRNA, β-thal: β-thalassemia, qRT-PCR: quantitative real-time PCR. Data expressed as mean ± standard deviation (SD). Compared with healthy controls, *ppnsp>0.05.</p

The hematological parameters of healthy controls and pediatric β-thal patients.

The hematological parameters of healthy controls and pediatric β-thal patients.</p

BCL11A expression was negatively regulated by hsa-miR-190b-5p.

(A, B) qRT-PCR and Western blot analysis of the mRNA and protein expression levels of BCL11A in 293T cells after transfected hsa-miR-190b-5p mimics and negative control mimics. β-actin gene was used as the internal control. (C) hsa-miR-190b-5p expression was decreased in 293T cells by hsa-miR-190b-5p inhibitors. (D,E) qRT-PCR and Western blot analysis of the mRNA and protein expression levels of BCL11A in 293T cells after transfected with hsa-miR-190b-5p inhibitors and negative control inhibitors. Data expressed as mean ± SD. Compared with negative control mimics or negative control inhibitors, *ppp<0.001.</p

hsa-miR-190b-5p as a diagnostic biomarker for pediatric β-thal patients.

ROC curves analysis of the AUC values in HbA2 alone (A), hsa-miR-190b-5p alone (B), and combination of both (C) for discriminating pediatric β-thal patients from healthy controls. ROC: receiver operating characteristic, AUC: the area under curve.</p

The relationship between hsa-miR-190b-5p expression and hematological parameters in pediatric β-thal patients without transfusion.

The relationship between hsa-miR-190b-5p expression and hematological parameters in pediatric β-thal patients without transfusion.</p

Emulsion Synthesis of Size-Tunable CH₃NH₃PbBr₃ Quantum Dots: An Alternative Route toward Efficient Light-Emitting Diodes

We report a facile nonaqueous emulsion synthesis of colloidal halide perovskite quantum dots by controlled addition of a demulsifier into an emulsion of precursors. The size of resulting CH₃NH₃PbBr₃ quantum dots can be tuned from 2 to 8 nm by varying the amount of demulsifier. Moreover, this emulsion synthesis also allows the purification of these quantum dots by precipitation from the colloidal solution and obtains solid-state powder which can be redissolved for thin film coating and device fabrication. The photoluminescence quantum yields of the quantum dots is generally in the range of 80–92%, and can be well-preserved after purification (∼80%). Green light-emitting diodes fabricated comprising a spin-cast layer of the colloidal CH₃NH₃PbBr₃ quantum dots exhibited maximum current efficiency of 4.5 cd/A, power efficiency of 3.5 lm/W, and external quantum efficiency of 1.1%. This provides an alternative route toward high efficient solution-processed perovskite-based light-emitting diodes. In addition, the emulsion synthesis is versatile and can be extended for the fabrication of inorganic halide perovskite colloidal CsPbBr₃ nanocrystals

BCL11A was a target of hsa-miR-190b-5p.

(A) The human BCL11A mRNA 3’-UTR harbored two putative binding sites (499–506 loci: ACATATC; 2553–2559 loci: ACATATC) of hsa-miR-190b-5p. These mutant binding sites (ACATATC>TGTATAG) were shown below. (B) qRT-PCR analysis of hsa-miR-190b-5p expression in 293T cells after transfected with hsa-miR-190b-5p mimics and negative control mimics. (C) Luciferase reporter assay analysis of the relative luciferase activity in 293T cells co-transfected with the WT2 or MUT2 reporter vector and hsa-miR-190b-5p mimics or negative control mimics. (D) hsa-miR-190b-5p mimics had no significant effect on the relative luciferase activity of WT1 and MUT1 reporter vectors. WT: wild type, MUT: mutant. Data expressed as mean ± SD. Compared with negative control mimics, *ppnsp>0.05.</p