13 research outputs found
Additional file 2 of Whole-genome sequencing and functional annotation of pathogenic Paraconiothyrium brasiliense causing human cellulitis
Additional file 2: Table S2. The secretome of P. brasiliense GGX 41
Additional file 1 of Whole-genome sequencing and functional annotation of pathogenic Paraconiothyrium brasiliense causing human cellulitis
Additional file 1: Table S1. KEGG functional annotation of P. brasiliense GGX 41
RNAi inhibits viral protein.
<p>RD cells transfected with siRNA were infected with strain EV71-2006-52-9 at an MOI of 0.01. At 36 h postinfection, total protein was extracted and analyzed with western blotting. β-Actin was used as the internal loading control. The protein measurements were made in three independent experiments.</p
Sequences of the synthesized siRNA molecules and their positions in the EV71 2A<sup>pro</sup> genomic region.
<p>Sequences of the synthesized siRNA molecules and their positions in the EV71 2A<sup>pro</sup> genomic region.</p
RNAi inhibits viral RNA.
<p>RD cells were treated with each siRNA and then infected with strain EV71-2006-52-9 at an MOI of 0.01. At 24 h postinfection, the viral RNA was extracted and analyzed with real-time RT–PCR. The data shown represent the means ± SD of three independent experiments (**p < 0.01).</p
Transfection efficiency of siRNA in RD cells.
<p>(A) Cellular distribution of BLOCK-iT Fluorescent Oligo in transfected RD cells. RD cells were transfected with different concentrations of BLOCK-iT Fluorescent Oligo and 2 μl of Lipofectamine 2000. At 24 h after transfection, the cells were observed under a fluorescence microscope. (B) RD cells transfected with BLOCK-iT Fluorescent Oligo were quantified with flow cytometry. The cells were assayed in three independent experiments.</p
siRNAs protect cells against EV71-induced cytopathic effects.
<p>Morphological changes in RD cells were observed after infection. Cells were transfected with each siRNA at a final concentration of 60 nM and then infected with strain EV71-2006-52-9 at an MOI of 0.01. Micrographs were taken at 48 h postinfection under an inverted microscope. The tests were performed in three independent experiments.</p
RNAi inhibits the replication of different EV71 strains.
<p>RD cells transfected with siRNA were infected with strain EV71-2008-43-16 at an MOI of 0.01. Total protein was extracted at 36 h after infection, and VP1 expression was analyzed with western blotting. β-Actin was used as the internal loading control. Protein measurements were made in three independent experiments.</p
Viral titer assay.
<p>Virus titers were determined in term of TCID<sub>50</sub>. RD cells pretreated with siRNAs were infected with strain EV71-2006-52-9 at an MOI of 0.01. At 48 h postinfection, the supernatants were collected to detect the progeny viral titers. Data are the means ± SD of three independent experiments (**p < 0.01).</p
Distribution of the outbreak strains and onset time.
<p>Distribution of the outbreak strains and onset time.</p