3 research outputs found
Quantitative Proteomics and Cytology of Rice Pollen Sterol-Rich Membrane Domains Reveals Pre-established Cell Polarity Cues in Mature Pollen
Cell
polarity is essential for generating diverse cell functions.
The underlying mechanisms of how a cell establishes, maintains, and
changes its polarity are poorly understood. Recently, sterol-rich
membrane microdomains are found to be associated with these processes.
However, both its exact characteristics and importance are still unclear.
Here we show microdomains change dynamically in developing and germinating
rice pollen with selective enrichment in the aperture and the tip
of newly born pollen tubes by use of the sterol-specific probe filipin.
Using the sterol extraction sensitivities of microdomain proteins
and quantitative proteomics, we identified 237 microdomain-associated
proteins from 934 identified pollen detergent resistant membrane proteins.
This proteome includes almost all of the known key regulators comprising
the polar growth network, and it shows more similarity to front–back
polarized HeLa cells than nonpolarized <i>Arabidopsis</i> suspension cells. We immunolocalize flotilin-like protein, a representative
of these sterol-dependent proteins and directly visualize microdomains
in pollen. These results indicate the presence of microdomains in
pollen and pre-established cell polarity around the aperture during
pollen maturation. Our findings reveal an atlas of the microdomain-associated
proteome in pollen. This work provides useful resources and knowledge
needed to further dissect the mechanisms for the establishment and
maintenance of cell polarity
Quantitative Proteomics and Cytology of Rice Pollen Sterol-Rich Membrane Domains Reveals Pre-established Cell Polarity Cues in Mature Pollen
Cell
polarity is essential for generating diverse cell functions.
The underlying mechanisms of how a cell establishes, maintains, and
changes its polarity are poorly understood. Recently, sterol-rich
membrane microdomains are found to be associated with these processes.
However, both its exact characteristics and importance are still unclear.
Here we show microdomains change dynamically in developing and germinating
rice pollen with selective enrichment in the aperture and the tip
of newly born pollen tubes by use of the sterol-specific probe filipin.
Using the sterol extraction sensitivities of microdomain proteins
and quantitative proteomics, we identified 237 microdomain-associated
proteins from 934 identified pollen detergent resistant membrane proteins.
This proteome includes almost all of the known key regulators comprising
the polar growth network, and it shows more similarity to front–back
polarized HeLa cells than nonpolarized <i>Arabidopsis</i> suspension cells. We immunolocalize flotilin-like protein, a representative
of these sterol-dependent proteins and directly visualize microdomains
in pollen. These results indicate the presence of microdomains in
pollen and pre-established cell polarity around the aperture during
pollen maturation. Our findings reveal an atlas of the microdomain-associated
proteome in pollen. This work provides useful resources and knowledge
needed to further dissect the mechanisms for the establishment and
maintenance of cell polarity
Quantitative Proteomics and Cytology of Rice Pollen Sterol-Rich Membrane Domains Reveals Pre-established Cell Polarity Cues in Mature Pollen
Cell
polarity is essential for generating diverse cell functions.
The underlying mechanisms of how a cell establishes, maintains, and
changes its polarity are poorly understood. Recently, sterol-rich
membrane microdomains are found to be associated with these processes.
However, both its exact characteristics and importance are still unclear.
Here we show microdomains change dynamically in developing and germinating
rice pollen with selective enrichment in the aperture and the tip
of newly born pollen tubes by use of the sterol-specific probe filipin.
Using the sterol extraction sensitivities of microdomain proteins
and quantitative proteomics, we identified 237 microdomain-associated
proteins from 934 identified pollen detergent resistant membrane proteins.
This proteome includes almost all of the known key regulators comprising
the polar growth network, and it shows more similarity to front–back
polarized HeLa cells than nonpolarized <i>Arabidopsis</i> suspension cells. We immunolocalize flotilin-like protein, a representative
of these sterol-dependent proteins and directly visualize microdomains
in pollen. These results indicate the presence of microdomains in
pollen and pre-established cell polarity around the aperture during
pollen maturation. Our findings reveal an atlas of the microdomain-associated
proteome in pollen. This work provides useful resources and knowledge
needed to further dissect the mechanisms for the establishment and
maintenance of cell polarity