Study on effects of Beta-glucan & extraction of Sargassum & Padina algae in prevention and treatment of WSD in Fenneropenaeus indicus

In this study two species of algae, Sargassum glaucescens and Padina boergeseni that found plenteously in Persian Gulf and Bushehr coast, were collected and hot water extracts of them were lyophilized. F. indicus (11.32±1.20 g), after two weeks adaptation in Shoghab research station were immersed in seawater (39 ppt and 25±1 ○C) containing hot-water extract of each brown algae, S.glaucescens and P.boergeseni, at 100, 300 and 500 mg/l concentration, Survival rate and immunological parameters (total haemocyte count (THC), total plasma protein (TPP), Phagocytic activity, bacterial clearance efficiency and bactericidal activity) were examined. In addition effect of dietary administration of beta 1, 3 1, 6 glucan on prevention of White Spot Disease and immunological parameters of shrimp were investigated. According to results, immersion in seawater containing 300 and 500 mg/l concentration of algal hot-water extract after 2 and 3 hours or oral administration of beta 1,3 1,6 glucan at level of 10 g/kg diet for 14 days significantly enhanced THC, TPP, Phagocytic activity, bacterial clearance efficiency and bactericidal activity. Immersion in seawater containing 100, 300 and 500 mg/l hot-water extract of S.glaucescens after 3 hours, improved the survival rate of WSSV-infected F. indicus

Introduction of Litopenaeus vannamei to shrimp culture industry of Iran

Various Problems in front of shrimp culture, mainly in biological fields and restrictions of production cycle, emphasized the species diversity in aquaculture industry of Iran in fourth socio- economic development plan of fisheries department. By this means, the Iranian Fisheries Research Organization, defined a Programme with four sub- projects, and started the activities on beginning of 2004. On the year 2004, 80 pairs of SPF/SPR Litopenaeus vannamei broodstocks imported from Hawaii, but late importation and prolonged acclimatization period, caused propogation and PL production on mid-September. Despite of terminated culture season, some PL's stocked in earthen ponds of Helleh site in Bushehr province. Results of this phase showed that, L.vannamei could support the cold winter of about 9 C temperature and about 20000 broodstocks harvested at the next year. At the next year, 55 pairs of SPF/SPR broodstocks imported by the same way, and after production of PL in Bandargah hatchery, the farming carried out in Helleh research earthen ponds. At this year, shrimp farms of Bushehr province infected with WSSV, but L.vannamei growth was very good, and gained more than19g after 90days farming. On the year 2006, the farming pilot project repeated, and the way for extension smoothed. In a parallel action, introduction of L.vannamei to Choebdeh site (Khouzestan province) showed remarkable results after 5 years collapses by WSSV. The results of broodstocking, breeding and farming research projects transferred very soon to shrimp producers, as the production of L.vannamei in Khouzestan and Bushehr provinces raised to 2575tons in the year 2008. In addition the condition for introduction of this species to Golestan province smoothed on 2008, and stocking at rate of 5-6 individuals per m2 in earthen ponds of Gomishan resulted in 20g. Shrimp weight after 75 days culture. This stock used as brood stock for commercial production on 2009 by private sector. It seems that all four southern provinces are going to utilize L.vannamei as the main species in 2009, and domesticated brood stocks

Quantitative and qualitative development of shrimp culture in Iran

Primary researches on aquaculture of endemic shrimp species carried out since 1984-5 by Iranian Fisheries Research Organization (IFRO) .After a decade, the condition for extension and development of shrimp culture in the country prepared. Despite huge investment of government for preparation and construction of shrimp sites, only small part of present potential in southern coast of the country is under production. About 18 different shrimp species inhabit in Persian Gulf and Sea of Oman, in which 4 species have larger size, and more abundant, and harvest commercially . Penaeus semisulcatus, Fenneropenaeus merguiensis and Metapenaeus affinis as commercial species distributed throughout of Persian Gulf from mouth of Arvand River in north- west to strait of Hormoz, and also some parts of Oman Sea. While Fenneropenaeus indicus distributed mainly in coastal area of Oman Sea. Shrimp culture begin in Iran by P.semisulcatus, continued by imported Penaeus monodon, and developed by using of F.indicus. After confront to slow growth and viral diseases in 2001 (Khouzestan province) and 2005 (Bushehr Province), at the same time I.F.R.O acquired the bio-technique for production of Litopenaeus vannamei, and hence the condition for introduction the new species to the shrimp culture industry provided. In accordance for development of shrimp culture, I.F.R.O carried out various research activities, and established specific research center for this purpose. Research projects regarding brood-stock management, breeding and larvae production, grow out, water management and disease identification and treatment have been carried, and induced considerable impacts on quantitative and qualitative development of shrimp culture in the country

Investigation of the influence of different level of plant protein (30, 50 and 70 percent) in Litopenaeus vannamei shrimp diet, with 38 percent protein

The influence of different percent’s of plant protein (30, 50 and 70) in the diet of white leg shrimp (Litopenaeus vannamei) with 38 percent protein, in comparison with a commercial shrimp diet (Fenneropenaeus indicus) with 39 percent protein (contain20 percent plant protein) was studied. Initial weight of shrimp was 10±1 gram. The average increase of shrimp weight in the end of culture period in experimental treatment 1 (contain 30 percent plant protein and 70 percent animal protein), 5.89±0.06 gram, experimental treatment 2 (contain 50 percent plant protein and 50 percent animal protein), 6.22±0.25 gram, experimental treatment 3 (contain 70 percent plant protein and 30 percent animal protein) 6/19±0/24 gram and in the control treatment (contain 20 percent plant protein and 80 percent animal protein) 6/42±0/40 were measured. Generally in the study of influence of experimental diets and control diet on the growth rate(GR), food conversion rate (FCR), protein efficiency ratio (PER), specific growth rate (SGR), average daily gain (ADG), demonstrate that, mentioned parameters in the control treatment is better than experimental treatments. But no significant difference measured between them (p>0/05). Also results showed that, application of diets contain 50 percent of plant protein (with 22 percent soybean meal), and 70 percent of plant protein (with 72 percent soybean meal), can decrease costs of diet preparation and therefore shrimp production

Study on brood stock management of Litopenaeus vannamei using imported post larvae

In order to domesticate Litopenaeus vannamei as a solution to single species shrimp aquaculture of Penaeus indicus and to reduce risk of shrimp culture in emergency cases, this study was carried out. Initially the postlarvae reared from imported L. vannamei were cultured. After culture season the adult shrimps were transported to greenhouse ponds for wintering. It is then relocated to hatchery saloon. After eye stalk ablation and maturation the female brood stocks were introduced to matured male for copulation at ponds. During the study period, male and female shrimp’s attained full maturation at end – April by temperature over 25ºC, the copulation process was started significantly. Finally of the 12 instances of copulation, one instance resulted nauplii production. The best maturation was observed at 28 ֯C ±1 and salinity30 to 33PPT and pH of 8 to 8.3 Fresh sea worms and squid showed the highest effect on fecundity in brood stocks. Besides, the spring season was obtained to be the best period for brood stock production in Bushehr province

Comparative analysis of the growth and survival of protozoa stages of Penaeus semisulcatus fed with four phytoplankton species

Four genera of phytoplankton including Tetrasehnis, Chaetoceros, Skeletonema and Chlorella were used as food for protozoa stages of Penaeus semisulcatus in 10 treatments each with three replications of singular and double diets. In singular diet consisted of 40,000 cells/ml of Skeletonema, we recorded a survival rate 82% growth rate 3.55 mm and period of development stage (Z I-MI) 92h which were higher than other treatments. In double diet comprised of Chaetoceros and Tetrasehnis, the survival rate in Zl stage 97% higher than other treatments while in Z2 and Z3 stages, the highest survival rate was 9500, achieved by a double diet consisted of Skeletonema and Chaetoceros. In the latter diet, the growth rate and period of development for Z I-MI stages and growth rates in singular diets are higher than double diets of the phytoplankton species