Sorrow's family : a novella

Miranda is trying to establish her own identity and build a rewarding relationship with her sister. But she must come to terms with the breakup of her marriage and her mother's suicide

Minimal information for studies of extracellular vesicles (MISEV2023): From basic to advanced approaches

Extracellular vesicles (EVs), through their complex cargo, can reflect the state of their cell of origin and change the functions and phenotypes of other cells. These features indicate strong biomarker and therapeutic potential and have generated broad interest, as evidenced by the steady year-on-year increase in the numbers of scientific publications about EVs. Important advances have been made in EV metrology and in understanding and applying EV biology. However, hurdles remain to realising the potential of EVs in domains ranging from basic biology to clinical applications due to challenges in EV nomenclature, separation from non-vesicular extracellular particles, characterisation and functional studies. To address the challenges and opportunities in this rapidly evolving field, the International Society for Extracellular Vesicles (ISEV) updates its 'Minimal Information for Studies of Extracellular Vesicles', which was first published in 2014 and then in 2018 as MISEV2014 and MISEV2018, respectively. The goal of the current document, MISEV2023, is to provide researchers with an updated snapshot of available approaches and their advantages and limitations for production, separation and characterisation of EVs from multiple sources, including cell culture, body fluids and solid tissues. In addition to presenting the latest state of the art in basic principles of EV research, this document also covers advanced techniques and approaches that are currently expanding the boundaries of the field. MISEV2023 also includes new sections on EV release and uptake and a brief discussion of in vivo approaches to study EVs. Compiling feedback from ISEV expert task forces and more than 1000 researchers, this document conveys the current state of EV research to facilitate robust scientific discoveries and move the field forward even more rapidly

THE COMPUTER: AN ASSESSMENT OF TEACHER TRAINING, TEACHER ATTITUDES, AND COMPUTER INVENTORY (HARDWARE AND SOFTWARE) IN GRADES K-8 IN THE METROPOLITAN PUBLIC SCHOOLS OF NASHVILLE-DAVIDSON COUNTY (TENNESSEE)

Data were gathered from teachers through a questionnaire concerning their attitudes and training of computers. The questionnaire contained demographic data, three openended questions, and the Minnesota Computer Literacy and Awareness Assessment, Form 8, Part I, consisting of twenty items. Teachers were asked to either strongly agree, agree, be undecided, disagree, or strongly disagree to the statements. Data were also gathered from appointed school computer coordinators through an assessment form concerning equipment, software, location of computers, and their perception of computer use in the schools. The findings revealed that experience in years of teaching appeared to have no significance in relationship to positive attitudes of teachers towards computers. Positive attitudes were found in teachers and coordinators who had previous training on computers. They wanted to learn more about the computer and to be on school computer committees. Many of these teachers owned their own computers. The teachers with training usually provided their students with more computer time. Trained teachers reflected more enjoyment in computers, and more confidence in their ability to use computers. More positive attitudes were reflected in teachers and coordinators who were under 40 years of age and who had higher degrees of learning. They had positive feelings toward the integration of computers into the educational system and revealed less anxiety in working with computers. Teachers\u27 comments on the open-ended questions revealed needs for more computers, more software, released time for training, computer laboratories with teachers, and numerous other concerns. Recommendations included further studies of computer programs in other school systems, a staff for the system-wide computer coordinator, better communications from the school system concerning seminars and availability of help and information for the teachers, released time for training, a system of sharing and previewing software, a computer laboratory with a teacher for each school, and the development of a system-wide computer program with a curriculum for grades K-6. Other recommendations were suggestions to interview the teachers for their needs, seek out skilled teachers in computer science and hire them, and use available laboratories within the system for seminars

Minimal information for studies of extracellular vesicles (MISEV2023): From basic to advanced approaches

Extracellular vesicles (EVs), through their complex cargo, can reflect the state of their cell of origin and change the functions and phenotypes of other cells. These features indicate strong biomarker and therapeutic potential and have generated broad interest, as evidenced by the steady year-on-year increase in the numbers of scientific publications about EVs. Important advances have been made in EV metrology and in understanding and applying EV biology. However, hurdles remain to realising the potential of EVs in domains ranging from basic biology to clinical applications due to challenges in EV nomenclature, separation from non-vesicular extracellular particles, characterisation and functional studies. To address the challenges and opportunities in this rapidly evolving field, the International Society for Extracellular Vesicles (ISEV) updates its ‘Minimal Information for Studies of Extracellular Vesicles’, which was first published in 2014 and then in 2018 as MISEV2014 and MISEV2018, respectively. The goal of the current document, MISEV2023, is to provide researchers with an updated snapshot of available approaches and their advantages and limitations for production, separation and characterisation of EVs from multiple sources, including cell culture, body fluids and solid tissues. In addition to presenting the latest state of the art in basic principles of EV research, this document also covers advanced techniques and approaches that are currently expanding the boundaries of the field. MISEV2023 also includes new sections on EV release and uptake and a brief discussion of in vivo approaches to study EVs. Compiling feedback from ISEV expert task forces and more than 1000 researchers, this document conveys the current state of EV research to facilitate robust scientific discoveries and move the field forward even more rapidly