Additional file 3: of A gel-based PCR method to differentiate sheeppox virus field isolates from vaccine strains

Figure S2. Determination of the limits of detection of the PCR assay. Defined amount for the plasmid genotype standard (104, 103, 100, 80, 60, 40, 20, 10, 1 and 0) for SPPV vaccine (A) and SPPV field isolates (B) were tested in parallel reactions and run on agarose gel. (PDF 77 kb

Additional file 2: of A gel-based PCR method to differentiate sheeppox virus field isolates from vaccine strains

Figure S1. Gel picture of PCR products for the remaining capripoxvirus samples. These sample were tested in this study, but not presented in Fig. 2 of the manuscript. The PCR products of 218 bp, 302 bp and 338 bp represent SPPV vaccine strains, SPPV field isolates/GTPVs, and LSDVs respectively. First row: MM = 50 bp DNA ladder; a = positive control plasmid of the SPPV field isolates; b = positive control plasmid of the SPPV vaccine strain; c = Negative control; Lane 5 to 15 (sample 23 to 33 in Table 1 of the manuscript). Second row: MM = 50 bp; Lane 2 to 14 (sample 34 to 46 in Table 1 of the manuscript). (PDF 104 kb

Additional file 4: of A gel-based PCR method to differentiate sheeppox virus field isolates from vaccine strains

Figure S3. Gel picture of PCR for the non-capripoxvirus samples tested in this study. MM = 50 bp DNA ladder; a = positive control plasmid of the SPPV field isolates; b = positive control plasmid of the SPPV vaccine strain; c = Negative control; 1–5 (ORF viruses); 6 (BPSV); 7–8 (Mccp); 9 (cDNA, PPRV); 10 (BOHV-1); 11 (BOHV-2). (PDF 45 kb

Additional file 1: of A gel-based PCR method to differentiate sheeppox virus field isolates from vaccine strains

Table S1. Non-capripoxvirus samples tested for specificity study. (DOCX 16 kb

EQA for RVFV virus genome detection, RNA extraction and RT PCR instruments and methods.

<p>na = not available information</p><p>* Laboratory providing multiple datasets</p><p>**Friedrich-Loeffler-Institut (in-house) using primer sequences published by Bird et al. (2007)</p><p>EQA for RVFV virus genome detection, RNA extraction and RT PCR instruments and methods.</p

Distribution of the correct results of laboratories participating to the EQAs for RVF antibodies detection (IgG and IgM).

<p>Group 1 includes laboratories, which correctly classified 15 out of the 15 tested samples, Group 2 represents the laboratory failing to one test result. For IgG EQA group 1 includes the laboratories: #1, #2, #3, #4, #5, #6, #7a, #8, #9, #9a, #9b, #10, while in group 2 #7. For IgM EQA group 1 includes #1, #2, #4, #5, #6, #7, #7a, #8, #9, #9a, #9b, #10 and group 2 #3.</p

Results of the EQA for RVFV virus genome detection.

<p>Neg: RVFV seronegative bovine serum. + / -: samples identified as positive or negative by the participants. FP: false positive result. FN: false negative result. Inc: Inconclusive results.</p><p>* Laboratory providing multiple datasets</p><p>Results of the EQA for RVFV virus genome detection.</p

Results of the EQA for RVF IgM antibodies detection.

<p>Wild rum: wild ruminants (<i>Antidorcasmarsupialis</i>). Pos: RVF seropositive status; Neg: RVF seronegative status. + /—: samples identified as positive or negative by the participants. FP: false positive</p><p>^<i>†</i>IDvet: ID. Screen RVF IgM Capture</p><p>^<i>§</i><i>In-house assay</i>: test based on crude cell lysate as antigen</p><p>* Laboratory providing multiple datasets</p><p>Results of the EQA for RVF IgM antibodies detection.</p

Results of the EQA for RVFV virus genome detection, cycle threshold (Ct) values.

<p>Neg: RVFV seronegative bovine serum. No Ct: result above the threshold.</p><p>* Laboratory providing multiple datasets</p><p>Results of the EQA for RVFV virus genome detection, cycle threshold (Ct) values.</p

Distribution of the correct results of laboratories participating to EQA for RVFV genome detection.

<p>Group 1 represents laboratories which correctly classified 15 out of the 15 tested samples (#1a, #7, #8, #9, #10). Group 2 represents the laboratory failing to one test result (#1). Group 3 represents the laboratory, which misidentified 5 test results (#2).</p