Intratumoral delivery of IL-18 naked DNA induces T-cell activation and Th1 response in a mouse hepatic cancer model-3

<p><b>Copyright information:</b></p><p>Taken from "Intratumoral delivery of IL-18 naked DNA induces T-cell activation and Th1 response in a mouse hepatic cancer model"</p><p>http://www.biomedcentral.com/1471-2407/7/87</p><p>BMC Cancer 2007;7():87-87.</p><p>Published online 23 May 2007</p><p>PMCID:PMC1903361.</p><p></p>edium alone or that containing CT26 lysates. Numbers within the gates in the FACS plots depict the percentage of CD69IFN-γCD4cells. Data are presented from experiments that were repeated at least twice

Intratumoral delivery of IL-18 naked DNA induces T-cell activation and Th1 response in a mouse hepatic cancer model-2

<p><b>Copyright information:</b></p><p>Taken from "Intratumoral delivery of IL-18 naked DNA induces T-cell activation and Th1 response in a mouse hepatic cancer model"</p><p>http://www.biomedcentral.com/1471-2407/7/87</p><p>BMC Cancer 2007;7():87-87.</p><p>Published online 23 May 2007</p><p>PMCID:PMC1903361.</p><p></p>smid DNA was injected directly into the tumors on day 7. Tumor growth was measured on days 1, 4, 7, and 14 after plasmid implantation. Data are presented as the mean of tumor weights of 8 mice/group

Intratumoral delivery of IL-18 naked DNA induces T-cell activation and Th1 response in a mouse hepatic cancer model-4

<p><b>Copyright information:</b></p><p>Taken from "Intratumoral delivery of IL-18 naked DNA induces T-cell activation and Th1 response in a mouse hepatic cancer model"</p><p>http://www.biomedcentral.com/1471-2407/7/87</p><p>BMC Cancer 2007;7():87-87.</p><p>Published online 23 May 2007</p><p>PMCID:PMC1903361.</p><p></p>, and cultured with anti-CD3 and anti-CD28 antibodies for 3 days before performing ELISA (* = 0.006)

Intratumoral delivery of IL-18 naked DNA induces T-cell activation and Th1 response in a mouse hepatic cancer model-1

<p><b>Copyright information:</b></p><p>Taken from "Intratumoral delivery of IL-18 naked DNA induces T-cell activation and Th1 response in a mouse hepatic cancer model"</p><p>http://www.biomedcentral.com/1471-2407/7/87</p><p>BMC Cancer 2007;7():87-87.</p><p>Published online 23 May 2007</p><p>PMCID:PMC1903361.</p><p></p>f CT26 tumor-bearing mice treated with plasmids were harvested at 7 days after plasmid injection, and cells analyzed by flow cytometry using the corresponding FITC- or PE- or PE-Cy5-labeled antibodies and isotype control

Intratumoral delivery of IL-18 naked DNA induces T-cell activation and Th1 response in a mouse hepatic cancer model-5

<p><b>Copyright information:</b></p><p>Taken from "Intratumoral delivery of IL-18 naked DNA induces T-cell activation and Th1 response in a mouse hepatic cancer model"</p><p>http://www.biomedcentral.com/1471-2407/7/87</p><p>BMC Cancer 2007;7():87-87.</p><p>Published online 23 May 2007</p><p>PMCID:PMC1903361.</p><p></p>oses of control plasmid or that expressing IL-18 until day 7 after tumor inoculation. Liver tissue was obtained on day 4 after direct injection of IL-18 plasmid DNA, and ELISA used to evaluate IL-18 protein levels. Representative data determined from two separate experiments

Induction of HI Abs upon immunization with Delta H1N1.

<p>On the day before challenge as described in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0039921#pone-0039921-g003" target="_blank">Figure 3</a>, the sera were collected and the titers of HI Abs were determined against A/PR8 virus. The values represent the mean + SEM antibody titers of sera from 5 mice per group.</p

SL immunization with DelNS1 vaccine activates mucosal and extramucosal CD4+ T cells.

<p>CFSE-labeled CD4⁺ T cells from HA-TCR transgenic mice were adoptively transferred into naïve mice. One day later, recipients were given SL 2×10⁷ pfu of Delta H1N1, 2×10⁵ pfu of mouse-adapted wt live or 40 µg of formalin-inactivated A/PR8. Three days after the immunization, proliferating (CFSE stained) HA-TCR CD4⁺ T cells were detected in cervical lymph nodes (CLN), lungs, mediastinal lymph nodes (MdLN) and spleens by FACS analysis. The data are representative of two experiments showing similar results.</p

Levels of virus-specific IgG induced in mucosal and systemic compartments upon immunization with DeltaNS1 IAV.

<p>BALB/c mice were immunized with different doses of Delta H1N1 (▴ H1N1) or wt live virus A/PR8/34 (PR8) H1N1 (WT) via the sublingual (SL) or intranasal (IN) route. Four weeks later, titers of H1N1 (A/PR8) virus-specific IgG in sera and secretions were determined by ELISA. The values represent mean + SEM ELISA titers determined on groups of 5 mice.</p

Induction of virus-specific IgG and IgA upon immunization with Delta H5N1.

<p>BALB/c mice were immunized with different doses of Delta H5N1 (▴ H5N1) or wt live virus A/PR8/34 (PR8) H1N1 via the sublingual (SL), nasal (N) or intranasal (IN) route. Four weeks later sera were collected and the levels of H5N1 virus-specific IgG and IgA were determined by Delta H5N1 virus-coated ELISA plates. The values represent the mean + SEM (vertical bars) end point ELISA antibody titers determined on 5 mice per group.</p

Levels of virus-specific IgA induced in mucosal and systemic compartments upon immunization with DeltaNS1 IAV.

<p>BALB/c mice were immunized as described in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0039921#pone-0039921-g006" target="_blank">Figure 6</a>. Four weeks later, titers of H1N1 (A/PR8) virus-specific IgA in sera and secretions were determined by ELISA. The values represent mean + SEM ELISA titers determined on groups of 5 mice.</p