A scientific note on the partial nucleotide sequence of a US strain of Kashmir bee virus isolated from Apis mellifera L.

International audienc

A scientific note on the detection of Kashmir bee virus in individual honeybees and Varroa jacobsoni mites

International audienc

Comparative stability of oxytetracycline and tylosin in sugar syrup

Measurements of OTC stability in apicultural dosage forms have relied on methods (fluorescence, bactericidal effects) that are not specific to OTC. We have measured the stability of OTC and tylosin in sucrose syrup using high-performance liquid chromatography. At 34 °C in the dark, OTC in syrup made from highly purified sucrose had a half life of 7.6 days. Tylosin was considerably more stable, with a half life of 186 days; the half life of the total tylosin complex was 287 days. In syrup prepared from commercial-grade sugar, both materials were less stable, with OTC having a half life of 6.3 days and tylosin about 75 days. Microbiological data paralleled these findings, with a rapid decrease of inhibition zone against Paenibacillus larvae with OTC, and a persistence of inhibition with tylosin. © Inra/DIB/AGIB/Elsevier, Pari

A scientific note on the identification of honey bee semen using a mitochondrial DNA marker

Note scientifique sur l'identification du sperme de l'abeille domestique à l'aide d'un marqueur d'ADN mitochondrial

Tracking an invasive honey bee pest: mitochondrial DNA variation in North American small hive beetles

We describe the current and past distributions of North American small hive beetles (Aethina tumida) having two distinct mitochondrial DNA haplotypes. A collection of 539 hive beetles showed irregular distributions of these haplotypes across the southeastern US. Beetles from the first collections made in coastal South Carolina showed haplotype NA1, exclusively. This haplotype is less common in Georgia and was not observed in North Carolina. Later collections from this and other parts of South Carolina appear more similar to those found in other states. The body size of beetles was not correlated with their haplotype, suggesting that differences in haplotype frequency do not reflect selection pressures on covarying differences in the genomes of these beetles. We discuss the implications for inferring the number of separate hive beetle introductions to the US, and for estimating the migration dynamics by hive beetles as they expand their ranges in the New World

Analysis of Africanized honey bee mitochondrial DNA reveals further diversity of origin

Within the past 40 years, Africanized honey bees spread from Brazil and now occupy most areas habitable by the species Apis mellifera, from Argentina to the southwestern United States. The primary genetic source for Africanized honey bees is believed to be the sub-Saharan honey bee subspecies A. m. scutellata. Mitochondrial markers common in A. m. scutellata have been used to classify Africanized honey bees in population genetic and physiological studies. Assessment of composite mitochondrial haplotypes from Africanized honey bees, using 4 base recognizing restriction enzymes and COI-COII intergenic spacer length polymorphism, provided evidence for a more diverse mitochondrial heritage. Over 25% of the "African" mtDNA found in Africanized populations in Argentina are derived from non-A. m. scutellata sources.<br>Nos últimos 40 anos, abelhas africanizadas se espalharam a partir do Brasil e agora ocupam a maioria das áreas habitáveis pela espécie Apis mellifera, da Argentina ao sudoeste dos Estados Unidos. Acredita-se que a fonte genética primária das abelhas africanizadas seja a subespécie subsaariana de abelha A. m. scutellata. Marcadores mitocondriais comuns em A. m. scutellata têm sido usados para classificar abelhas africanizadas em estudos de fisiologia e genética de população. A avaliação de haplótipos mitocondriais compostos em abelhas africanizadas, usando 3 enzimas de restrição e um polimorfismo de comprimento no espaçador intergênico "COI-COII", evidenciou uma herança mitocondrial mais diversa. Mais de 25% do mtDNA "africano" encontrado em populações africanizadas na Argentina são derivados de fontes não relacionadas a A. m. scutellata