THE IMPLEMENTATION OF ROLE PLAY IN TEACHING SPEAKING NARRATIVE TEXT FOR EIGHTH GRADERS OF SMPN 1 SUMBERREJO BOJONEGORO

Abstrak Penelitian ini dibuat untuk menggambarkan penerapan tekhnik role play untuk pengajaran berbicara teks naratif untuk kelas 8 SMPN 1 Sumberrejo Bojonegoro. Penelitian ini mengambil beberapa permasalahan: (1) bagaimana guru menerapkan tekhnik role play dalam mengajar berbicara teks naratif untuk kelas 8? (2) bagaimana respon siswa terhadap tekhnik role play dalam pembelajaran berbicara teks naratif?. Berdasarkan penelitian yang telah di lakukan, bisa di simpulkan pertama bahwa guru bahasa Inggris yang menerapkan role play dalam mengajar berbicara teks naratif menggunakan langkah yang sangat baik. Langkah- langkah yang di gunakan guru yang pertama memberikan materi yang simple kepada siswa. Kemudian guru mengajarkan dialog-dialognya. Sebelum maju di depan kelas, guru menyuruh siswa untuk memperaktekkan terlebih dahulu di dalam groupnya masing-masing. Guru juga membolehkan siswa merubah dialog untuk membuat variasi-variasi. Untuk kesimpulan yang kedua, siswa memberikan respon yang positif terhadap penerapan role play dalam pengajaran berbicara naratif teks. Meskipun beberapa dari mereka merasa sulit dalam melakukan role play, mereka mengatakan role play memotivasi untuk berbicara bahasa Inggris. Kata kunci: Pengajaran berbicara, teks naratif dan role play. Abstract This study is designed to describe the implementation of role play in teaching speaking narrative text for eighth graders SMPN 1 Sumberrejo Bojonegoro. This study composes some questions: (1) how does the teacher implement role play in teaching speaking narrative text for eighth graders? (2) how are the students’ responses toward the implementation of role play in teaching speaking narrative text? teaching and learning process of writing narrative text through ROLE PLAY technique?. Based on the study, they are the conclusions; first, the teacher implements role play in teaching speaking narrative text very well. She gives the simple materials and the simple dialogues. The teacher teaches the dialogues and has the students to practice before performing in front of the class. She also allows them to modify the dialogues to make a variation. Second, the students give positive response toward the implementation of role-play in teaching speaking narrative text. Although some students still get difficulty in doing role play. They said that role-play is able to increase students’ motivation to speak English. Key words: Teaching speaking, narrative text and role play

Pilot solar desalination plants in Bangladesh

Pilot solar desalination plants in Banglades

Toxorhynchites-fluorescent antibody system for the detection of bluetongue virus from Culicoides midges (Diptera: Ceratopogonidae)

A new system, the Toxorhynchites-fluorescent antibody (TFA) test in which the larvae of Toxorhynchites splendens mosquitoes were used for the detection of bluetongue virus (BTV) from Culicoides midges, was developed. Twenty-seven pools of Culicoides midges were collected from bluetongue-prone areas of Tamil Nadu by use of the light-trap and suction-trap methods. A suspension of each pool was injected intrathoracically into T. splendens IV instar larvae and inoculated onto Vero cell monolayers. An indirect fluorescent antibody technique and an immunoperoxidase test were used to detect BTV antigen in smears of crushed midges, crushed larval head smears after incubation for 7 d at 28° and cell monolayers showing cytopathic effects 48 h post inoculation. The suspensions were also injected intravenously into embryonated chicken eggs, and the characteristic BTV-induced lesion(s), viz. cherry-red appearance of embryos, were observed after 48 h. Virus was confirmed by a qualitative neutralization test conducted simultaneously in embryonated chicken eggs. A total of seven out of 27 samples (26%) were positive for the presence of BTV antigen in all the diagnostic systems used. Since BTV propagates readily in experimentally infected T. splendens larvae and the BTV antigen can be detected by the fluorescent antibody technique with a sensitivity comparable to that for virus propagated in tissue culture and embryonated eggs, the TFA system can be adopted as a new method for the isolation of BTV from vectors. The advantages of the TFA system are discussed.The articles have been scanned in colour with a HP Scanjet 5590; 600dpi. Adobe Acrobat X Pro was used to OCR the text and also for the merging and conversion to the final presentation PDF-format

Prevalence of extended spectrum β-lactamase, AmpC β-lactamase and metallo β-lactamase mediated resistance in Escherichia coli from diagnostic and tertiary healthcare centers in south Bangalore, India

Background: The increasing reports on multidrug resistant Escherichia coli has become a potential threat to global health. Here, we present a cross-sectional study to characterize extended spectrum β-lactamase, AmpC β-lactamase and metallo β-lactamase producing E. coli isolated from different human clinical samples.Methods: A total of 300 clinical Gram negative bacterial isolates were collected and re-characterized for the identification of E. coli following standard microbiological techniques. The antimicrobial susceptibility of E. coli isolates was initially screened by Kirby-Bauer disk diffusion and MIC methods. The resistant isolates were confirmed to be ESBL, AmpC and MBL producers by their respective phenotypic confirmatory tests of combined disc method.Results: We identified 203 (68%) E. coli and 97 (32%) Non-E. coli isolates. The highest recovery of E. coli was from urine samples 72 (35%). Combined disc method using ceftazidime/ceftazidime+clavulanic acid and cefotaxime/cefotaxime+clavulanic acid confirmed 156 (79%) and 144 (73%) E. coli as ESBL producers, respectively. Thirty-four (34%) and 16 (27%) resistant E. coli isolates were confirmed to be AmpC and MBL producers, likewise.Conclusions: Increased prevalence of ESBL, AmpC and MBL producing E. coli were observed. Beta-lactamase mediated resistance appears to be prime mechanism in the multidrug resistant E. coli. Thus, early detection of beta lactamase producing E. coli is necessary to avoid treatment failure and prevent the spread of MDR

A hybrid automata framework for an adaptive impedance control of a robot-assisted training system

There is an increasing demand for an effective and adaptive robot-assisted training system for traumatic brain injury patients which can considerably promote their sensorimotor control performance, apart from ensuring the safety of the patients. This study focuses on the impedance control framework to simultaneously track the position trajectory while regulating the apparent impedance of the robot. The framework is based on the hybrid automata model that is used to govern the desired trajectory deployed by the robot-assisted training in assisting rehabilitative motion. A designed experimental setup was developed to evaluate the performance of the proposed hybrid automata scheme. Preliminary simulation results demonstrated the excellent response of the proposed framework with its ability to track the desired trajectory as well as the varying patients’ arm impedance profile. © Springer Nature Singapore Pte Ltd. 2020

Insight into the dehydration behaviour of scandium-substituted barium titanate perovskites via simultaneous in situ neutron powder thermodiffractometry and thermogravimetric analysis

Hydration-dehydration cycles are critical to the mechanical performance of ceramic proton conductors. The development of in situ methods is desirable in order to study their structural response under conditions that mimic the operating ones. Neutron powder diffraction studies combined with simultaneous thermogravimetric analysis were performed on the hydrated forms of two members of the oxygen deficient perovskite BaTi1−xScxO3−δseries, with x = 0.5 and x = 0.7. Rietveld analyses agreed with in situ gravimetric data, allowing correlation of occupancy factors of the oxygen site to hydration levels and other structural data. Dehydration is an activated process that impacts on structural parameters and the level of Sc substitution was found to control the structural response during in situ dehydration, with higher Sc content leading to significantly greater volume contraction. This was rationalised by the chemical expansion due to hydration of oxygen vacancies within the x = 0.5 sample being anomalously small. Furthermore, the behaviour of the x = 0.5 system revealed an unexpected cell expansion during the early stages of dehydration, suggesting the hydration level may influence the thermal expansion coefficient (TEC)

MyD88 TIR domain higher-order assembly interactions revealed by microcrystal electron diffraction and serial femtosecond crystallography.

MyD88 and MAL are Toll-like receptor (TLR) adaptors that signal to induce pro-inflammatory cytokine production. We previously observed that the TIR domain of MAL (MALTIR) forms filaments in vitro and induces formation of crystalline higher-order assemblies of the MyD88 TIR domain (MyD88TIR). These crystals are too small for conventional X-ray crystallography, but are ideally suited to structure determination by microcrystal electron diffraction (MicroED) and serial femtosecond crystallography (SFX). Here, we present MicroED and SFX structures of the MyD88TIR assembly, which reveal a two-stranded higher-order assembly arrangement of TIR domains analogous to that seen previously for MALTIR. We demonstrate via mutagenesis that the MyD88TIR assembly interfaces are critical for TLR4 signaling in vivo, and we show that MAL promotes unidirectional assembly of MyD88TIR. Collectively, our studies provide structural and mechanistic insight into TLR signal transduction and allow a direct comparison of the MicroED and SFX techniques

MyD88 TIR domain higher-order assembly interactions revealed by microcrystal electron diffraction and serial femtosecond crystallography.

MyD88 and MAL are Toll-like receptor (TLR) adaptors that signal to induce pro-inflammatory cytokine production. We previously observed that the TIR domain of MAL (MALTIR) forms filaments in vitro and induces formation of crystalline higher-order assemblies of the MyD88 TIR domain (MyD88TIR). These crystals are too small for conventional X-ray crystallography, but are ideally suited to structure determination by microcrystal electron diffraction (MicroED) and serial femtosecond crystallography (SFX). Here, we present MicroED and SFX structures of the MyD88TIR assembly, which reveal a two-stranded higher-order assembly arrangement of TIR domains analogous to that seen previously for MALTIR. We demonstrate via mutagenesis that the MyD88TIR assembly interfaces are critical for TLR4 signaling in vivo, and we show that MAL promotes unidirectional assembly of MyD88TIR. Collectively, our studies provide structural and mechanistic insight into TLR signal transduction and allow a direct comparison of the MicroED and SFX techniques