42 research outputs found
Detection of Borrelia-specific 16S rRNA sequence in total RNA extracted from Ixodes ricinus ticks
A reverse transcriptase - polymerase chain reaction based assay for Borrelia species detection in ticks was developed. The method was based on amplification of 552 nucleotide bases long sequence of 16S rRNA, targeted by Borrelia specific primers. In the present study, total RNA extracted from Ixodes ricinus ticks was used as template. The results showed higher sensitivity for Borrelia detection as compared to standard dark-field microscopy. Method specificity was confirmed by cloning and sequencing of obtained 552 base pairs long amplicons. Phylogenetic analysis of obtained sequences showed that they belong to B. lusitaniae and B. afzelii genospecies. RT-PCR based method presented in this paper could be very useful as a screening test for detecting pathogen presence, especially when in investigations is required extraction of total RNA from ticks
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Absorption cross sections and kinetic considerations of the IO radical as determined by laser flash photolysis/laser absorption spectroscopy
Independent measurements of the absorption cross section of the IO radical for the A/sup 2/II-X/sup 2/II band system are reported. A value of (3.1 +- 0.6) x 10/sup -17/ cm/sup 2/ was observed for the (4,0) bandhead in good agreement with an earlier investigation. Results are also reported for the (5,0), (3,0), (2,0), and (1,0) bands over the wavelength range of 410-470 nm. However, no measurable (.. products (3) has been measured at 760 Torr of N/sub 2/ and 300 K. The measured effective bimolecular rate constant was found to be (6.6 +- 2) x 10/sup -11/ cm/sup 3/ x molecule/sup -1/ x s/sup -1/, in good agreement with recent results by Sander (1986)
Comparison of Macroinvertebrate-Derived Stream Quality Metrics Between Snag and Riffle Habitats 1
Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/74406/1/j.1752-1688.2008.00197.x.pd