10 research outputs found
Filamentous actin accumulates during plant cell penetration and cell wall plug formation in Phytophthora infestans
Get PDFCell proliferation, cell shape, and microtubule and cellulose microfibril organization of tobacco BY-2 cells are not altered by exposure to near weightlessness in space
Full text linkLive cell imaging of arabidopsis root hairs
No full textRoot hairs are tubular extensions from the root surface that expand by tip growth. This highly focused type of cell expansion, combined with position of root hairs on the surface of the root, makes them ideal cells for microscopic observation. This chapter describes the method that is routinely used in our laboratory for live cell imaging of Arabidopsis root hairs.</p
Optical trapping in plant cells
No full textOptical tweezers allow for noninvasive manipulation of subcellular compartments to study their physical interactions and attachments. By measuring (delay of) displacements, (semi)quantitative force measurements within a living cell can be performed. In this chapter, we provide practical tips for setting up such experiments paying special attention to the technical considerations for integrating optical tweezers into a confocal microscope. Next, we describe experimental approaches we have taken to trap intracellular structures in plant cells.</p
A new kymogram-based method reveals unexpected effects of marker protein expression and spatial anisotropy of cytoskeletal dynamics in plant cell cortex
No full textIn vivo mouse and live cell STED microscopy of neuronal actin plasticity using far-red emitting fluorescent proteins
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BABY BOOM target genes provide diverse entry points into cell proliferation and cell growth pathways
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