NAD-Independent L-Lactate Dehydrogenase Is Required for L-Lactate Utilization in Pseudomonas stutzeri SDM

BACKGROUND: Various Pseudomonas strains can use L-lactate as their sole carbon source for growth. However, the L-lactate-utilizing enzymes in Pseudomonas have never been identified and further studied. METHODOLOGY/PRINCIPAL FINDINGS: An NAD-independent L-lactate dehydrogenase (L-iLDH) was purified from the membrane fraction of Pseudomonas stutzeri SDM. The enzyme catalyzes the oxidation of L-lactate to pyruvate by using FMN as cofactor. After cloning its encoding gene (lldD), L-iLDH was successfully expressed, purified from a recombinant Escherichia coli strain, and characterized. An lldD mutant of P. stutzeri SDM was constructed by gene knockout technology. This mutant was unable to grow on L-lactate, but retained the ability to grow on pyruvate. CONCLUSIONS/SIGNIFICANCE: It is proposed that L-iLDH plays an indispensable function in Pseudomonas L-lactate utilization by catalyzing the conversion of L-lactate into pyruvate

Erratum to:magnetic configuration effects on the Wendelstein 7-X stellarator (Nature Physics, (2018), 14, 8, (855-860), 10.1038/s41567-018-0141-9)

\u3cp\u3eIn the version of this Article originally published, A. Mollén’s affiliation was incorrectly denoted as number 10; it should have been 1. Throughout the Article, some technical problems in typesetting meant that the tilde symbol above b and one instance of a superscript 2 were too high to be visible; see the correction notice for details. Finally, the citation to ref. \u3csup\u3e35\u3c/sup\u3e on page one of the Supplementary Information was incorrect; it should have been to ref. 36. These issues have now been corrected.\u3c/p\u3

Magnetic configuration effects on the Wendelstein 7-X stellarator

\u3cp\u3e The two leading concepts for confining high-temperature fusion plasmas are the tokamak and the stellarator. Tokamaks are rotationally symmetric and use a large plasma current to achieve confinement, whereas stellarators are non-axisymmetric and employ three-dimensionally shaped magnetic field coils to twist the field and confine the plasma. As a result, the magnetic field of a stellarator needs to be carefully designed to minimize the collisional transport arising from poorly confined particle orbits, which would otherwise cause excessive power losses at high plasma temperatures. In addition, this type of transport leads to the appearance of a net toroidal plasma current, the so-called bootstrap current. Here, we analyse results from the first experimental campaign of the Wendelstein 7-X stellarator, showing that its magnetic-field design allows good control of bootstrap currents and collisional transport. The energy confinement time is among the best ever achieved in stellarators, both in absolute figures (τ \u3csub\u3eE\u3c/sub\u3e > 100 ms) and relative to the stellarator confinement scaling. The bootstrap current responds as predicted to changes in the magnetic mirror ratio. These initial experiments confirm several theoretically predicted properties of Wendelstein 7-X plasmas, and already indicate consistency with optimization measures. \u3c/p\u3