5 research outputs found
Heterologous induction of a predicted promoter sequence for paraquat-inducible genes of Chromobacterium violaceum in response to paraquat compound
Growth inhibition of Prevotella ruminicola by protamine
Growth of Prevotella ruminicola strains B(1)4 (subsp. brevis) and D31d (subsp. ruminicola), was inhibited by protamine, a polycationic, low molecular mass protein. Results showed that protamine has a bacteriocidal effect when present in concentrations exceeding 30 mu g ml(-1). Protamine exerted its toxic effects by disrupting the outer membrane, which was demonstrated by: (i) an increased sensitivity to hydrophobic antibiotics (novobiocin and monensin) and (ii) release of the periplasmic enzyme alkaline phosphatase following short-term exposure to protamine. Although the concentrations of protamine inhibitory to P. ruminicola are relatively low, the effects of such a compound are probably too broad to permit its successful use in terms of manipulating ruminal proteolysis