``Agro-meteorological indices and climate model uncertainty over the UK''

Five stakeholder-relevant indices of agro-meteorological change were analysed for the UK, over past (1961--1990) and future (2061--2090) periods. Accumulated Frosts, Dry Days, Growing Season Length, Plant Heat Stress and Start of Field Operations were calculated from the E-Obs (European Observational) and HadRM3 (Hadley Regional Climate Model) PPE (perturbed physics ensemble) data sets. Indices were compared directly and examined for current and future uncertainty. Biases are quantified in terms of ensemble member climate sensitivity and regional aggregation. Maps of spatial change then provide an appropriate metric for end-users both in terms of their requirements and statistical robustness. A future UK is described with fewer frosts, fewer years with a large number of frosts, an earlier start to field operations (e.g., tillage), fewer occurrences of sporadic rainfall, more instances of high temperatures (in both the mean and upper range), and a much longer growing season

Molecular Epidemiological Investigation of Plasmodium knowlesi in Humans and Macaques in Singapore

Singapore reported its first locally acquired human Plasmodium knowlesi infection in 2007, involving a soldier who had undergone training in a forested area where long-tailed macaques are frequently seen. Comprehensive disease surveillance and monitoring system that was set up after the initial case detected four additional human P. knowlesi cases in 2007 and one in 2008. All involved military personnel who had undergone training in the forested area, and none had traveled out of Singapore 1 month before the onset of symptoms. Screening for malaria parasites on blood obtained from long-tailed macaques revealed that wild monkeys (n = 3) caught from the forested area were infected with P. knowlesi, whereas peri-domestic monkeys (n = 10) caught from a nature reserve park were not infected with any malaria parasites. Phylogenetic analysis of the nonrepeat region of the P. knowlesi csp genes showed that the sequences obtained from the human cases were not distinct from those obtained from wild monkeys. Further, certain genotypes were shared between samples from humans and macaques. Our findings provide evidence that long-tailed macaques are the natural hosts of P. knowlesi in Singapore and the human cases acquired their infection in the same vicinity where these monkeys are found. Further, the risk of acquiring P. knowlesi infection among the general population of Singapore is small as evident from the absence of P. knowlesi in peri-domestic monkeys