胎盤抽出物がヒト骨肉腫細胞株Saos-2の細胞増殖、I型コラーゲン産生およびALP分泌に及ぼす効果

Porcine placenta extract (P-placenta) is widely applied in medicine and cosmetics. However, few studies have examined the effect of the extract on the cellular behavior of the osteoblastic cell line Saos-2. Here, we demonstrated that P-placenta enhances the proliferation, collagen type I production, and alkaline phosphatase (ALP) secretion of Saos-2 in vitro. Proliferation of Saos-2 was assessed by MTT and DNA synthesisassays. Type I collagen production and ALP secretion were evaluated using enzyme-linked immunosorbent assay and ALP assays. The cells were treated with/without 20, 200 and 2000 g/ml of P-placenta for 24 h. We found that 200 g/ml P-placenta significantly induced the proliferation of Saos-2 and enhanced type I collagen production and ALP secretion. The results indicate that P-placenta controls the cellular behavior of osteoblasts,resulting in the secretion of early bone-related biomarkers

子宮頸癌に対するアポトーシス誘導を介した治療戦略に関する研究

学位の種別: 課程博士審査委員会委員 : （主査）東京大学教授 岡 明, 東京大学准教授 甲賀 かをり, 東京大学講師 長野 宏一朗, 東京大学講師 池村 雅子, 東京大学講師 山下 英臣University of Tokyo(東京大学

市販茶系飲料の示すフリーラジカル消去活性

Tea drinks, prepared from the tea plant classified as Camellia sinensis, contain antioxidative phenolic compounds, like catechins and flavonols. We evaluated the free radical scavenging activities of tea drinks on the market by measuring luminol-amplified chemiluminescence stimulated by the free radical initiator 2,2'-azobis (2-amidinopropane9 dihydrochloride, and the absorption of 2,2'-azinobis (3-ethylbenzothiazoline-6-sulphonic acid) radica cation at 734 nm. Black tea drinks and green tea drinks mostly showed high free radical scavenging activities in either assay, followed in order by oolong tea drinks and blend tea drinks that contain extracts from plants other than the tea plant. Total phenol contents in tea drinks highly correlated with their free radical scavenging activities, indicating polyphenols in tea drinks are the major components contributing to the free radical scavenging activities of tea drinks

Value of late gadolinium enhancement by magnetic resonance in patients with cardiac sarcoidosis: characteristic findings and clinical utility

73rd Annual Scientific Meeting of the Japanese Circulation Society 2009年03月 Osak

Graduate Students’ Learning in Yogo-practice Research

　「発達支援研究特論Ⅴ（養護教育実践研究）」は，大学で学んだ理論を学校現場で実証し，養護実践研究を行う 能力を身に付けることを目標にした授業科目である。大学院生３名の附属小・中学校における取組と課題の検討 を通じて，学びと保健室経営への参画について考察した。その結果，大学院生は異なる事例の検討を通じて，様々 な角度から課題を発見し，その対策を示すことができた。また，事例検討の過程で，自分自身が一定の役割を果 たすこと，より広い視野を持つ必要性，一人の養護教諭としての自覚をより強く持つ必要があること等に気付く ことができた。大学教員の課題として，養護実践のふりかえりの頻度を増やし，よりきめ細かくフィードバック すること等が挙げられた。このような取組は養護教諭としての資質能力の向上に有用であると考えられた。今後 は大学院生が自覚的，主体的に養護実践に取り組み，学びを検討し，共有する機会の充実が求められる

Value of late gadolinium enhancement by magnetic resonance in patients with cardiac sarcoidosis: characteristic findings and clinical utility

73rd Annual Scientific Meeting of the Japanese Circulation Society 2009年03月 Osak

Glycolysis Inhibition Inactivates ABC Transporters to Restore Drug Sensitivity in Malignant Cells

Cancer cells eventually acquire drug resistance largely via the aberrant expression of ATP-binding cassette (ABC) transporters, ATP-dependent efflux pumps. Because cancer cells produce ATP mostly through glycolysis, in the present study we explored the effects of inhibiting glycolysis on the ABC transporter function and drug sensitivity of malignant cells. Inhibition of glycolysis by 3-bromopyruvate (3BrPA) suppressed ATP production in malignant cells, and restored the retention of daunorubicin or mitoxantrone in ABC transporter-expressing, RPMI8226 (ABCG2), KG-1 (ABCB1) and HepG2 cells (ABCB1 and ABCG2). Interestingly, although side population (SP) cells isolated from RPMI8226 cells exhibited higher levels of glycolysis with an increased expression of genes involved in the glycolytic pathway, 3BrPA abolished Hoechst 33342 exclusion in SP cells. 3BrPA also disrupted clonogenic capacity in malignant cell lines including RPMI8226, KG-1, and HepG2. Furthermore, 3BrPA restored cytotoxic effects of daunorubicin and doxorubicin on KG-1 and RPMI8226 cells, and markedly suppressed subcutaneous tumor growth in combination with doxorubicin in RPMI8226-implanted mice. These results collectively suggest that the inhibition of glycolysis is able to overcome drug resistance in ABC transporter-expressing malignant cells through the inactivation of ABC transporters and impairment of SP cells with enhanced glycolysis as well as clonogenic cells

Use of Cap Analysis Gene Expression to detect human papillomavirus promoter activity patterns at different disease stages

Transcription of human papillomavirus (HPV) genes proceeds unidirectionally from multiple promoters. Direct profiling of transcription start sites (TSSs) by Cap Analysis Gene Expression (CAGE) is a powerful strategy for examining individual HPV promoter activity. The objective of this study was to evaluate alterations of viral promoter activity during infection using CAGE technology. We used CAGE-based sequencing of 46 primary cervical samples, and quantitatively evaluated TSS patterns in the HPV transcriptome at a single-nucleotide resolution. TSS patterns were classified into two types: early promoter-dominant type (Type A) and late promoter-dominant type (Type B). The Type B pattern was more frequently found in CIN1 and CIN2 lesions than in CIN3 and cancer samples. We detected transcriptomes from multiple HPV types in five samples. Interestingly, in each sample, the TSS patterns of both HPV types were the same. The viral gene expression pattern was determined by the differentiation status of the epithelial cells, regardless of HPV type. We performed unbiased analyses of TSSs across the HPV genome in clinical samples. Visualising TSS pattern dynamics, including TSS shifts, provides new insights into how HPV infection status relates to disease state