Methods for RNA extraction, cDNA preparation and analysis of CFTR transcripts

The scope of this article is to outline some of the basic methods for good quality RNA preparation from mammalian tissues and cells (including epithelial cells). Additionally, we give an outline of common techniques of measuring CFTR gene expression such as quantitative and semi-quantitative reverse transcription (RT) PCR and ribonuclease protection assay (RPA). These methods are designed to detect low abundance transcripts, which apply to CFTR mRNA in most cell types and tissues. © 2004 European Cystic Fibrosis Society. Published by Elsevier B.V. All rights reserved