9 research outputs found
One-step separation system for the main phospholipids, glycolipids, and phenolics by normal phase HPLC. Application to polar lipid extracts from olive and sunflower oils
A new normal phase high performance liquid chromatographic system has
been developed that allows efficient separation or several polar lipids,
such as phospholipids, glycolipids, and phenolics.
By this method, hesperidin, naringin, cerebrosides, phosphatidylcholine,
sphingomyelin, platelet activating factor, lysophosphatidylcholine,
digalactosyldiglycerides, and phosphatidylethanolamine are separated
within 60 min.
A gradient elution with acetonitrile/methanol, methanol, and water was
performed with a normal phase aminopropyl-modified silica gel HPLC
column. The eluted lipids were monitored by UV detection.
In addition, in this study, a successful application of the new method
is represented for the polar lipids of olive oil and sunflower, which
are also identified with chemical determinations