70 research outputs found
Generation of Vascular Endothelial Cells and Hematopoietic Cells by Blastocyst Complementation.
In the case of organ transplantation accompanied by vascular anastomosis, major histocompatibility complex mismatched vascular endothelial cells become a target for graft rejection. Production of a rejection-free, transplantable organ, therefore, requires simultaneous generation of vascular endothelial cells within the organ. To generate pluripotent stem cell (PSC)-derived vascular endothelial cells, we performed blastocyst complementation with a vascular endothelial growth factor receptor-2 homozygous mutant blastocyst. This mutation is embryonic lethal at embryonic (E) day 8.5-9.5 due to an early defect in endothelial and hematopoietic cells. The Flk-1 homozygous knockout chimeric mice survived to adulthood for over 1 year without any abnormality, and all vascular endothelial cells and hematopoietic cells were derived from the injected PSCs. This approach could be used in conjunction with other gene knockouts which induce organ deficiency to produce a rejection-free, transplantable organ in which all the organ's cells and vasculature are PSC derived
A safeguard system for induced pluripotent stem cell-derived rejuvenated T cell therapy
The discovery of induced pluripotent stem cells (iPSCs) has created promising new avenues for therapies in regenerative medicine. However, the tumorigenic potential of undifferentiated iPSCs is a major safety concern for clinical translation. To address this issue, we demonstrated the efficacy of suicide gene therapy by introducing inducible caspase-9 (iC9) into iPSCs. Activation of iC9 with a specific chemical inducer of dimerization (CID) initiates a caspase cascade that eliminates iPSCs and tumors originated from iPSCs. We introduced this iC9/CID safeguard system into a previously reported iPSC-derived, rejuvenated cytotoxic T lymphocyte (rejCTL) therapy model and confirmed that we can generate rejCTLs from iPSCs expressing high levels of iC9 without disturbing antigen-specific killing activity. iC9-expressing rejCTLs exert antitumor effects in vivo. The system efficiently and safely induces apoptosis in these rejCTLs. These results unite to suggest that the iC9/CID safeguard system is a promising tool for future iPSC-mediated approaches to clinical therapy
Current status of space gravitational wave antenna DECIGO and B-DECIGO
Deci-hertz Interferometer Gravitational Wave Observatory (DECIGO) is the
future Japanese space mission with a frequency band of 0.1 Hz to 10 Hz. DECIGO
aims at the detection of primordial gravitational waves, which could be
produced during the inflationary period right after the birth of the universe.
There are many other scientific objectives of DECIGO, including the direct
measurement of the acceleration of the expansion of the universe, and reliable
and accurate predictions of the timing and locations of neutron star/black hole
binary coalescences. DECIGO consists of four clusters of observatories placed
in the heliocentric orbit. Each cluster consists of three spacecraft, which
form three Fabry-Perot Michelson interferometers with an arm length of 1,000
km. Three clusters of DECIGO will be placed far from each other, and the fourth
cluster will be placed in the same position as one of the three clusters to
obtain the correlation signals for the detection of the primordial
gravitational waves. We plan to launch B-DECIGO, which is a scientific
pathfinder of DECIGO, before DECIGO in the 2030s to demonstrate the
technologies required for DECIGO, as well as to obtain fruitful scientific
results to further expand the multi-messenger astronomy.Comment: 10 pages, 3 figure
Multiple therapeutic peptide vaccines consisting of combined novel cancer testis antigens and anti-angiogenic peptides for patients with non-small cell lung cancer
Stable Operation of a 300-m Laser Interferometer with Sufficient Sensitivity to Detect Gravitational-Wave Events within our Galaxy
TAMA300, an interferometric gravitational-wave detector with 300-m baseline
length, has been developed and operated with sufficient sensitivity to detect
gravitational-wave events within our galaxy and sufficient stability for
observations; the interferometer was operated for over 10 hours stably and
continuously. With a strain-equivalent noise level of , a signal-to-noise ratio (SNR) of 30 is expected for
gravitational waves generated by a coalescence of 1.4 -1.4
binary neutron stars at 10 kpc distance. %In addition, almost all noise sources
which limit the sensitivity and which %disturb the stable operation have been
identified. We evaluated the stability of the detector sensitivity with a
2-week data-taking run, collecting 160 hours of data to be analyzed in the
search for gravitational waves.Comment: 5 pages, 4 figure
Current status of space gravitational wave antenna DECIGO and B-DECIGO
The Deci-hertz Interferometer Gravitational Wave Observatory (DECIGO) is a future Japanese space mission with a frequency band of 0.1 Hz to 10 Hz. DECIGO aims at the detection of primordial gravitational waves, which could have been produced during the inflationary period right after the birth of the Universe. There are many other scientific objectives of DECIGO, including the direct measurement of the acceleration of the expansion of the Universe, and reliable and accurate predictions of the timing and locations of neutron star/black hole binary coalescences. DECIGO consists of four clusters of observatories placed in heliocentric orbit. Each cluster consists of three spacecraft, which form three Fabry–Pérot Michelson interferometers with an arm length of 1000 km. Three DECIGO clusters will be placed far from each other, and the fourth will be placed in the same position as one of the other three to obtain correlation signals for the detection of primordial gravitational waves. We plan to launch B-DECIGO, which is a scientific pathfinder for DECIGO, before DECIGO in the 2030s to demonstrate the technologies required for DECIGO, as well as to obtain fruitful scientific results to further expand multi-messenger astronomy
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