Insulin increases the cell number of the inner cell mass and stimulates morphological development of mouse blastocysts in vitro

Previous studies showed that insulin promotes cell proliferation and morphological development of preimplantation mouse embryos. In this report, the receptor responsible for these actions and the cell populations that are affected were investigated. Insulin's 9% stimulation of blastocyst cell number was entirely due to a 23% increase in ICM cell number with an EC50 of 0.54 pM. This and the similar degrees of stimulation of immunosurgically isolated ICMs by both physiological and supraphysiological insulin concentrations suggest that insulin receptors are present on the ICM and respond to exogenous insulin transcytosed through the TE to promote expansion of the ICM cell numbers. In morphological studies, insulin increased the number of blastocysts and decreased the number of morulae by 10% after 54 h culture from 2-cell embryos with EC50s of about 0.95 pM. The equivalence of these EC50s suggests mediation of insulin's stimulation of blastocyst formation via insulin receptors which are functionally expressed around the time of compaction at the 8-cell stage. These results support our hypothesis that insulin has an important role in the regulation of growth during preimplantation development

IGF-2 receptors are first expressed at the 2-cell stage of mouse development

A specific IGF-2 receptor antiserum was used to reveal the presence of IGF-2 receptors during preimplantation development of mice. Receptors were present on 2-, 4- and 8-cell embryos, morulae, blastocysts, and on ICMs isolated prior to staining. There was no evidence for receptors on fertilized eggs. These observations confirm reports of the expression of IGF-2 receptor mRNA as early as the 2-cell stage and refine similar observations in blastocysts to confirm expression in both the TE and ICM. A potential auto/paracrine loop is thus one of the first products of activation of the embryonic genome and is expressed constitutively through preimplantation development

The Self-Dual String and Anomalies in the M5-brane

We study the anomalies of a charge $Q_2$ self-dual string solution in the Coulomb branch of $Q_5$ M5-branes. Cancellation of these anomalies allows us to determine the anomaly of the zero-modes on the self-dual string and their scaling with $Q_2$ and $Q_5$. The dimensional reduction of the five-brane anomalous couplings then lead to certain anomalous couplings for D-branes.Comment: 13 pages, Harvmac, refs adde

Membrane solitons in eight-dimensional hyper-Kaehler backgrounds

We derive the BPS equations satisfied by lump solitons in $(2+1)$-dimensional sigma models with toric 8-dimensional hyper-K\"ahler (${HK}_8$) target spaces and check they preserve 1/2 of the supersymmetry. We show how these solitons are realised in M theory as M2-branes wrapping holomorphic 2-cycles in the \bE^{1,2}\times {HK}_8 background. Using the $\kappa$-symmetry of a probe M2-brane in this background we determine the supersymmetry they preserve, and note that there is a discrepancy in the fraction of supersymmetry preserved by these solitons as viewed from the low energy effective sigma model description of the M2-brane dynamics or the full M theory. Toric ${HK}_8$ manifolds are dual to a Hanany-Witten setup of D3-branes suspended between 5-branes. In this picture the lumps correspond to vortices of the three dimensional ${\mathcal N}=3$ or ${\mathcal N}=4$ theory.Comment: 12+1 pages. LaTex. v2: Typos corrected and references adde

A bound on 6D N=1 supergravities

We prove that there are only finitely many distinct semi-simple gauge groups and matter representations possible in consistent 6D chiral (1,0) supergravity theories with one tensor multiplet. The proof relies only on features of the low-energy theory; the consistency conditions we impose are that anomalies should be cancelled by the Green-Schwarz mechanism, and that the kinetic terms for all fields should be positive in some region of moduli space. This result does not apply to the case of the non-chiral (1,1) supergravities, which are not constrained by anomaly cancellation.Comment: 23 pages, no figures; two paragraphs added to the proof in Appendix A covering the SU(2) and SU(3) case, other minor correction

Attempts to identify Cassava Brown Streak Virus in western Democratic Republic of Congo

Open Access ArticleRoot necrosis similar to those of the cassava brown streak disease (CBSD) were observed on cassava in western provinces of the Democratic Republic of Congo (DR.Congo) in the early 2000’s. However molecular laboratory diagnosis were not able to detect any causative agent responsible for the attacks, hence, the disease related to these symptoms was named CBSD-like disease. In order to assess the distribution and the incidence of the CBSD-like disease, surveys were carried out in four western provinces, comprising, Kwango and Kwilu, Sud Ubangi, Kinshasa and Kongo Central. CBSD-like disease was observed in all surveyed provinces on the basis of root symptoms because foliar symptoms were different to those of the documented cases of CBSD in other parts of east Africa. CBSD-like disease incidence was high in Kongo Central and Sud Ubangi, exceeding an average of 50 %, but low in Kwango and Kwilu (32.8%) and in Kinshasa (19.1%). During the surveys, cassava leaf samples were collected for lab identification of the causal agent. PCR diagnosis was done on these samples using primers specific for the two known CBSVs. All samples tested negative with no amplification of DNA fragments of the correct size. Thus, further analysis on the causative organism is needed using Next Generation Sequencing (NGS) approaches. NGS approaches will help also to identify the causative organism in other Central Africa countries (Angola, Congo-Brazzaville and Gabon) where such cassava root necrosis have been reported or are suspected

Spin dependent D-brane interactions and scattering amplitudes in matrix theory

Spin interactions beteween two moving Dp-branes are analyzed using the Green-Schwarz formalism of boundary states. This approach turns out to be extremely efficient to compute all the spin effects related by supersymmetry to the leading v^4/r^7-p term. All these terms are shown to be scale invariant, supporting a matrix model description of supergravity interactions. By employing the LSZ reduction formula for matrix theory and the mentioned supersymmetric effective potential for D0-branes, we compute the t-pole of graviton-graviton and three form-three form scattering in matrix theory. The results are found to be in complete agreement with tree level supergravity in the corresponding kinematical regime and provide, moreover, an explicit map between these degrees of freedom in both theories.Comment: 8 pages, no figures, talk presented at the conference "Quantum aspects of gauge theories, supergravity and unification", Corfu, Greece, to appear in the proceeding

Gravitational couplings of orientifold planes

We reanalyse the gravitational couplings of the perturbative orientifold planes $Op^-$, $Op^+$ (and D-branes). We first compute their $D_{-1}$ instantonic corrections for $p=3$. Then, by using U-dualities, we obtain the Wess-Zumino terms of orientifolds with RR flux for $p \leq 5$. The expressions for the effective actions can be partially checked via M-theory. We point out a previous oversimplification and we show in fact that the difficulty still stands in the way of the full computation of 7 Brane instanton corrections.Comment: 13 pages, 1 figure, 2 tables. 3 references adde

Holographic Description of Gravitational Anomalies

The holographic duality can be extended to include quantum theories with broken coordinate invariance leading to the appearance of the gravitational anomalies. On the gravity side one adds the gravitational Chern-Simons term to the bulk action which gauge invariance is only up to the boundary terms. We analyze in detail how the gravitational anomalies originate from the modified Einstein equations in the bulk. As a side observation we find that the gravitational Chern-Simons functional has interesting conformal properties. It is invariant under conformal transformations. Moreover, its metric variation produces conformal tensor which is a generalization of the Cotton tensor to dimension $d+1=4k-1, k\in Z$. We calculate the modification of the holographic stress-energy tensor that is due to the Chern-Simons term and use the bulk Einstein equations to find its divergence and thus reproduce the gravitational anomaly. Explicit calculation of the anomaly is carried out in dimensions $d=2$ and $d=6$. The result of the holographic calculation is compared with that of the descent method and agreement is found. The gravitational Chern-Simons term originates by Kaluza-Klein mechanism from a one-loop modification of M-theory action. This modification is discussed in the context of the gravitational anomaly in six-dimensional $(2,0)$ theory. The agreement with earlier conjectured anomaly is found.Comment: 24 pages, Latex; presentation re-structured, new references adde

Supersymmetric Dissipative Quantum Mechanics from Superstrings

Following the approach of Callan and Thorlacius applied to the superstring, we derive a supersymmetric extension of the non-local dissipative action of Caldeira and Leggett. The dissipative term turns out to be invariant under a group of superconformal transformations. When added to the usual kinetic term, it provides an example of supersymmetric dissipative quantum mechanics. As a by-product of our analysis, an intriguing connection to the homeotic/hybrid fermion model, proposed for CPT violation in neutrinos, appears.Comment: Latex, 16 page