Promotion and Application of Innovative Technology in the Construction of Yunmao Expressway Green Road

With Yunfu Luoding city to Maoming Xinyi city highway as the support, builders focus on introducing innovative technology in the process of creating green road demonstration project, promoting energy conservation and emission reduction, reducing common quality problems, improving the technical level and management level of green road construction, and playing a reference role for the scientific application of innovative technology in common national and provincial highways

Fault line selection method for coal mine power grid based on RCMDE and KFCM

It is difficult to accurately select the fault line when the single-phase ground fault occurs in the coal mine power grid with the widely used resonant grounding system. In order to solve the above problem, a fault line selection method of the coal mine power grid based on the refined composite multiscale dispersion entropy (RCMDE) and the kernel fuzzy C-means clustering (KFCM) is proposed. The limitations of using amplitude, polarity and waveform similarity as line selection characteristic quantities: the applicability of the line selection method based on amplitude and polarity difference is limited. If the polarity of the zero sequence current transformer in the line is reversed, the method based on polarity will directly fail. When the sampling is not synchronized, the line selection method based on waveform similarity is difficult to obtain correct results. In order to overcome the above limitations, RCMDE is introduced to measure the complexity and irregularity of the transient zero sequence current signal of each line. RCMDE is used as the characteristic quantity of line selection. The KFCM algorithm is used to cluster the RCMDE to realize the automatic identification of fault lines. The bus fault and feeder fault are distinguished by judging whether the contour coefficient exceeds the threshold value. Finally, the feeder line with the fault point is judged through the membership degree matrix obtained by clustering. The simulation results show the following points. ① The RCMDE curve of the fault line is different from that of the non-fault line, and the curves can be divided into two types. RCMDE can be used as the fault characteristic index of fault line. ② When the bus fault occurs, there are clusters with an average contour coefficient less than the threshold value in the clustering results. However, when feeder fault occurs, the contour coefficients of the clustering results are all greater than the threshold value. Under various fault scenarios, the coal mine power grid fault line selection method based on RCMDE and KFCM can realize correct line selection. The results show that its accuracy is not affected by factors such as fault line, fault location, fault closing angle and grounding resistance. ③ Under the conditions of noise disturbance, the fault line selection method based on RCMDE and KFCM can realize correct line selection in the case of low resistance grounding or high resistance grounding. And the method has a strong anti-interference capability. ④ Under the conditions of asynchronous sampling and reverse polarity of zero-sequence current transformer in the fault line, the method based on RCMDE and KFCM can still realize correct line selection. And the line selection result has high robustness

Underground roadway shape variable monitoring algorithm based on laser radar

In order to solve the problem of correct monitoring of underground roadway shape variables, a monitoring algorithm of underground roadway shape variables based on laser radar is designed based on the installation of high-precision laser radar and vehicle aided positioning system in underground intelligent vehicle; the overall framework of the monitoring algorithm is established, and the key technologies in laser radar data acquisition and preprocessing are described in detail; based on the existing knowledge accumulation of underground roadway scene monitoring and around the feature extraction of discrete points, an improved special point registration algorithm is constructed, which solves the problem that the feature points of roadway scene are sparse and can not be registered effectively; the distance from point to surface is used to pick out outliers, quickly calculate the offset of points, quickly extract high-density outliers through the density clustering algorithm of DBSCAN, quickly screen the deformation area, analyze the deformation degree, and realize the intelligent deformation monitoring of underground roadway surface. The test results show that the roadway deformation monitoring algorithm based on laser radar can monitor the roadway surface shape changes under logging in real time, dynamically and accurately, and can ensure the normal operation of underground roadway and protect the safety of miners’ life and property

Transmission competence of a new mesonivirus, Yichang virus, in mosquitoes and its interference with representative flaviviruses.

Advances in technology have greatly stimulated the understanding of insect-specific viruses (ISVs). Unfortunately, most of these findings are based on sequencing technology, and laboratory data are scarce on the transmission dynamics of ISVs in nature and the potential effects of these viruses on arboviruses. Mesonivirus is a class of ISVs with a wide geographical distribution. Recently, our laboratory reported the isolation of a novel strain of mesonivirus, Yichang virus (YCV), from Culex mosquitoes, China. In this study, the experimental infection of YCV by the oral route for adult and larvae mosquitoes, and the vertical transmission has been conducted, which suggests that YCV could adopt a mixed-mode transmission. Controlled experiments showed that the infectivity of YCV depends on the mosquito species, virus dose, and infection route. The proliferation curve and tissue distribution of YCV in Cx. quinquefasciatus and Ae. albopictus showed that YCV is more susceptible to Ae. albopictus and is located in the midgut. Furthermore, we also assessed the interference of YCV with flaviviruses both in vitro and in vivo. YCV significantly inhibited the proliferation of DENV-2 and ZIKV, in cell culture, and reduced transmission rate of DENV-2 in Ae. albopictus. Our work provides insights into the transmission of ISVs in different mosquito species during ontogeny and their potential ability to interact with mosquito-borne viruses

Icariin Ameliorates Lower Back Pain in Rats via Suppressing the Secretion of Cytokine-Induced Neutrophil Chemoatractant-1

Purpose. To investigate whether icariin (ICA), a well-known medicine extracted from the stem and leaf of Epimedium brevicornum Maxim, had analgesic effect on lower back pain (LBP) in rats. Methods. In a puncture-induced LBP rat model, the severity of LBP was quantified using the paw/foot withdrawal threshold method after intragastric administration of ICA at a dosage of 50 mg/kg/d or 100 mg/kg/d. The pain-related peptides of substance P (SP) and calcitonin gene-related peptide (CGRP) were also measured in intervertebral disc (IVD) tissue using RT-PCR after ICA treatment. In addition, the expression of cytokine-induced neutrophil chemoattractant-1 (CINC-1) in IVD was quantified using RT-PCR and ELISA examination. Results. ICA treatment resulted in a significant amelioration of mechanical allodynia in a dose-response manner, and the analgesic effect could last for two weeks even during the washout period. More importantly, the mechanism of analgesic pharmacological effect in ICA was to suppress the upregulated CINC-1, the homolog of IL-8 in rats, which is a crucial proalgesic factor contributing to LBP, in IVDs. Conclusion. ICA is a novel herbal extract to relieve LBP, and it may be a promising alternative pain killer in the future

Clinical features and transmission risk analysis of dengue virus infections in Shenzhen, During 2014–2019

Dengue fever (DF) and dengue hemorrhagic fever (DHF) are among the most common tropical diseases affecting humans. To analyze the risk of clinical and transmission of DF/DHF in Shenzhen, the surveillance on patients of all-age patients with dengue virus (DENV) infections was conducted. Our findings revealed that the majority of DENV-infected patients are young to middle-aged males, and the development of the disease is accompanied by abnormal changes in the percentages of neutrophils, lymphocytes, and basophils. Demographic analysis revealed that these patients is concentrated in areas such as Futian District, which may be due to the higher mosquito density and temperature than that in other area. Subsequent, mosquito infection experiments confirmed that the effect of temperature shift on DENV proliferation and transmission. Not only that, constant temperatures can enhance the spread of DENV, even increase the risk of epidemic. Thus, the role of innate immune response should be highlighted in the prediction of severe severity of DENV-infected patients, and temperature should be taken into account in the prevention and control of DENV. Introduction: Dengue fever (DF) and dengue hemorrhagic fever (DHF) are among the most common tropical diseases affecting humans, and which caused by the four dengue virus serotypes (DENV 1–4). Objectives: To analyze the risk of clinical and transmission of DF/DHF in Shenzhen. Methods: The surveillance on patients of all-age patients with dengue virus (DENV) infections was conducted. Results: Our findings revealed that the majority of DENV-infected patients are young to middle-aged males, and the development of the disease is accompanied by abnormal changes in the percentages of neutrophils, lymphocytes, and basophils. Demographic analysis revealed that these patients is concentrated in areas such as Futian District, which may be due to the higher mosquito density and temperature than that in other area. Subsequent, mosquito infection experiments confirmed that the effect of temperature shift on DENV proliferation and transmission. Not only that, constant temperatures can enhance the spread of DENV, even increase the risk of epidemic. Conclusion: 1. Elevated levels of neutrophils, lymphocytes, basophils, and temperature are all significant risk factors for dengue transmission and pathogenesis; 2. Temperature increasing is associated with a higher risk of dengue transmission; 3. Fluctuations in temperature around 28 °C (28 ± 5 °C) would increase dengue transmission

Proteomic Insights into citT-Deletion Induced Metabolic Sensitivity in Bio-Degumming of Ramie Fibers by Bacillus subtilis

ABSTRACTCellulose fibers from ramie is a kind of functional biological material with multipurpose in the textile industry, which called as “China grass.” The degumming of ramie fibers performed with Bacillus subtilis were regulated by response regulator CitT from CitS/CitT two-component system through specifically functioning on the degradation of component pectin. In this study, comparative proteomic analysis was executed to obtain insights into the sensitivity by which the metabolic network induced by the absence of CitT protein, and to further explore the regulatory mechanism during bio-degumming process of ramie fibers. Results showed that 29 differentially expressed proteins were detected from original strain and mutant strain, which were mainly involved in transmembrane transport system, two-component system, and amino acid metabolism. This study demonstrated that the lack of CitT protein could result in the down-regulation of enzymes in histidine biosynthesis pathway, and the up-regulation of enzyme in arginine degradation pathway. This study is the first time to reveal comprehensive information about the regulatory function of CitT protein in bio-degumming of ramie fibers, and may provide important scientific and technological basis for targeted constructing engineering strains in degumming of ramie fibers

Significant effect of Ca modification on improving catalytic stability of Cu-catalyst in gas-phase furfural hydrogenation to furfuralcohol

The gas-phase hydrogenation of furfural to furfuralcohol over Cr-free Cu-based catalysts has attracted increasing attention due to its environmentally friendly nature and mild operating conditions. Although reduced pure nano-sized CuO exhibits complete furfural hydrogenation and nearly 100% furfuralcohol selectivity, it suffers from rapid deactivation caused by sintering. In this study, we conducted comparative investigations on the catalytic performance and stability of two Cu-based catalysts: 90%CuO-10%SiO2 and 90%CuO-5%CaO-5%SiO2, in the gas-phase furfural hydrogenation. The reaction is carried out under various conditions, including temperatures ranging from 120 to 170 ℃, LHSVs of 1 to 2.2 h−1, and H2 to furfural molar ratios of 3.5 to 12.5. The results indicate that under optimal conditions, the Ca-modified catalyst achieves nearly complete furfural conversion and almost 100% furfuralcohol selectivity for a test duration of 31 h. In contrast, the unmodified catalyst exhibits stable performance for only seven hours despite the similar initial performance. XRD analysis confirms that the gradual deactivation of both catalysts is attributed to the oxidation of reduced metallic Cu sites to Cu oxides. Further characterizations of the two spent catalysts using HRTEM and XPS analyses, along with DFT calculations, suggest that the presence of Ca in Cu lattices prevents the loss of electrons from low-valence Cu sites or the reduced metallic Cu sites, thus inhibiting their oxidation to high-valence Cu oxides. This phenomenon contributes to suppressing the deactivation of Cu-catalysts in the gas-phase furfural hydrogenation process

Coxsackievirus A16 elicits incomplete autophagy involving the mTOR and ERK pathways.

Autophagy is an important homeostatic process for the degradation of cytosolic proteins and organelles and has been reported to play an important role in cellular responses to pathogens and virus replication. However, the role of autophagy in Coxsackievirus A16 (CA16) infection and pathogenesis remains unknown. Here, we demonstrated that CA16 infection enhanced autophagosome formation, resulting in increased extracellular virus production. Moreover, expression of CA16 nonstructural proteins 2C and 3C was sufficient to trigger autophagosome accumulation by blocking the fusion of autophagosomes with lysosomes. Interestingly, we found that Immunity-related GTPase family M (IRGM) was crucial for the activation of CA16 infection-induced autophagy; in turn, reducing IRGM expression suppressed autophagy. Expression of viral protein 2C enhanced IRGM promoter activation, thereby increasing IRGM expression and inducing autophagy. CA16 infection inhibited Akt/mTOR signaling and activated extracellular signal-regulated kinase (ERK) signaling, both of which are necessary for autophagy induction. In summary, CA16 can use autophagy to enhance its own replication. These results raise the possibility of targeting the autophagic pathway for the treatment of hand, foot, and mouth disease (HFMD)

Measurement of the autophagic flux in HeLa cells infected with CA16.

<p>(A, B) Western blotting of cells with autophagy inhibited by CQ. HeLa cells were pretreated with CQ for 4 h, followed by infection with CA16 at an MOI of 2. After 1 h of virus absorption at 37°C, the cells were further cultured in maintenance medium in the absence or presence of CQ. At 24 h after infection with CA16, the cells were subjected to Western blotting using anti-LC3B and Vp1 antibodies. (C) Western blotting analysis of p62 protein expression in HeLa cells infected with CA16. Cells were infected with CA16 at an MOI of 1. After 1 h of virus absorption at 37°C, the cells were further cultured in maintenance medium. Cells were harvested at the indicated time points and detected with anti-p62 antibody compared to uninfected control cells. (D) HeLa cells transfected with ptfLC3 were infected with CA16 (MOI = 1) or treated with CQ or HBSS. The cells were collected, fixed, and visualized at 24 h postinfection. The graph shows the quantification of autophagosomes by calculating the average number of dots in 20 cells. Scale bar, 10μm. (E) Western blotting of autophagy-related proteins in cells transfected with the indicated shRNA and determination of CA16 replication in these transfected cells. HeLa cells were transfected with either specific shRNA targeting Beclin 1, Atg5 or scrambled shRNA. At 48 h after transfection, cells were infected with CA16 at an MOI of 2. Samples were collected at 12 h after infection with CA16 and detected with anti-Beclin 1,-Atg5 and-Vp1 antibodies. β-Actin was used as a protein loading control. The extracellular virus yields were determined at 48 hpi and expressed as lgTCID50/ml. Data are presented as the means from three independent experiments. Significance was analyzed with a two-tailed Student’s <i>t</i> test. *<i>P</i>< 0.05, **<i>P</i>< 0.01, ***<i>P</i>< 0.001. (F) Determination of CA16 replication in HeLa cells treated with CQ. HeLa cells were pretreated with CQ for 4 h followed by infection with CA16 at an MOI of 2. The extracellular virus yields were determined at 12 hpi and expressed as lgTCID50/ml. Data are presented as the means from three independent experiments. Significance was analyzed with a two-tailed Student’s <i>t</i> test. *<i>P</i>< 0.05.</p