Plasma membrane receptor mediated MAPK signaling pathways are activated in human uterine cervix at parturition

BACKGROUND: Cervical ripening resembles an inflammatory reaction. Estrogens induce leukocyte migration into tissue and factors promoting cervical remodeling and labor, although the mechanisms are only partially known. The aim of this study was to investigate whether plasma membrane receptor mediated pathways, known to be activated by estrogens and proinflammatory compounds, are involved in cervical ripening before labor. METHODS: The expression and distribution of mitogen activated protein kinases (MAPK), which transduce extracellular signals into intracellular responses through phosphorylation, and their intracellular targets transcription factors c-Jun and c-Fos proteins (AP-1) were analysed in cervical biopsies from term pregnant women (TP), immediately after parturition (PP), and from non-pregnant women (NP). Immunohistochemistry and RT-PCR techniques were used. RESULTS: Cell-specific alterations in the immunostaining pattern for MAPK were observed. The expressions of activated, phosphorylated MAPK forms pERK1/2, pJNK and p38MAPK were significantly increased in cervical stroma until TP and pERK1/2 expression was significantly enhanced in PP group. c-Jun was significantly increased in cervical stroma and smooth muscle in TP as compared to NP group. c-Fos was significantly increased in stroma, squamous epithelium and glandular epithelium in PP as compared to TP group. CONCLUSION: We report, for the first time, cell-specific activation of pMAPKs and their targets transcription factors c-Fos and c-Jun (AP-1) proteins in human uterine cervix until term pregnancy, and immediately after parturition. These results suggest a role for MAPK activation in cervical ripening before labor

Inhibition of extravillous trophoblast cell invasion in 3% oxygen is due to an increase in PAI-2 production

The origin of the submarine Naturaliste Plateau off the southwestern coast of Australia is controversial; previous work supports both oceanic and continental affinities for the basement to volcanic and sedimentary sequences. We report the first evidence of reworked Mesoproterozoic (ca. 1230–1190 Ma) continental crust, based on laser ablation–inductively coupled plasma–mass spectrometry analysis of zircons from granite and orthogneiss samples dredged from the southern margin of the plateau. Thermobarometry of peak metamorphic minerals and electron microprobe chemical dating of monazite reveal that these igneous rocks were metamorphosed to ~700 °C and ~6.5 kbar during the Cambrian Pinjarra Orogeny at ca. 515 Ma. These data confirm a continental origin for a significant swathe of the southern Naturaliste Plateau, and suggest that the protoliths may have affinities to Mesoproterozoic crust within the Albany-Fraser-Wilkes Orogen (Australia-Antarctica). The present Naturaliste Plateau basement beneath its volcanic carapace probably represents a middle-to lower-crustal extensional allochthon exhumed during Cretaceous hyperextensional breakup between Australia and Antarctica.<br/