998 research outputs found

    Rheological properties of aging thermosensitive suspensions

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    Aging observed in soft glassy materials inherently affects the rheological properties of these systems and has been described by the soft glassy rheology (SGR) model [S. M. Fielding et al., J. Rheol. 44, 323 (2000)]. In this paper, we report the measured linear rheological behavior of thermosensitive microgel suspensions and compare it quantitatively with the predictions of the SGR model. The dynamic moduli [G[prime](omega,t) and G[double-prime](omega,t)] obtained from oscillatory measurements are in good agreement with the model. The model also predicts quantitatively the creep compliance J(t−tw,tw), obtained from step stress experiments, for the short time regime [(t−tw)<tw]. The relative effective temperature [script X]/[script X]g obtained from both the oscillatory and the step stress experiments is indeed less than 1 ([script X]/[script X]g<1) in agreement with the definition of aging. Moreover, the elasticity of the compressed particles (Gp) increases with increased compression, i.e., the degree of hindrance and consequently also the bulk elasticity (G[prime] and 1/J) increases with the degree of compression

    Sexual development in the homothallic green alga Chlamydomonas monoica Strehlow

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    Direct Awards in Germany – Design And Effects

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    Institute of Transport and Logistics Studies. Faculty of Economics and Business. The University of Sydne

    What factors explain the number of physical therapy treatment sessions in patients referred with low back pain; a multilevel analysis

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    BACKGROUND: It is well-known that the number of physical therapy treatment sessions varies over treatment episodes. Information is lacking, however, on the source and explanation of the variation. The purposes of the current study are: 1) to determine how the variance in the number of physical therapy treatment sessions in patients with non-specific low back pain (LBP) in the Netherlands is distributed over patient level, therapist level and practice level; and 2) to determine the factors that explain the variance. METHODS: Data were used from a national registration network on physical therapy. Our database contained information on 1,733 patients referred with LBP, treated by 97 therapists working in 41 practices. The variation in the number of treatment sessions was investigated by means of multilevel regression analyses. RESULTS: Eighty-eight per cent of the variation in the number of treatment sessions for patients with LBP is located at patient level and seven per cent is located at practice level. It was possible to explain thirteen per cent of all variance. The duration of the complaint, prior therapy, and the patients' age and gender in particular are related to the number of physical therapy treatment sessions. CONCLUSION: Our results suggest that the number of physical therapy treatment sessions in patients with LBP mainly depends on patient characteristics. More variation needs to be explained, however, to improve the transparency of care. Future research should examine the contribution of psychosocial factors, baseline disability, and the ability to learn motor behavior as possible factors in the variation in treatment sessions

    Cloning and functional characterization of a fructan 1-exohydrolase (1-FEH) in edible burdock (Arctium lappa L.)

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    <p>Abstract</p> <p>Background</p> <p>We have previously reported on the variation of total fructooligosaccharides (FOS), total inulooligosaccharides (IOS) and inulin in the roots of burdock stored at different temperatures. During storage at 0°C, an increase of FOS as a result of the hydrolysis of inulin was observed. Moreover, we suggested that an increase of IOS would likely be due to the synthesis of the IOS by fructosyltransfer from 1-kestose to accumulated fructose and elongated fructose oligomers which can act as acceptors for fructan:fructan 1-fructosyltransferase (1-FFT). However, enzymes such as inulinase or fructan 1-exohydorolase (1-FEH) involved in inulin degradation in burdock roots are still not known. Here, we report the isolation and functional analysis of a gene encoding burdock 1-FEH.</p> <p>Results</p> <p>A cDNA, named <it>aleh1</it>, was obtained by the RACE method following PCR with degenerate primers designed based on amino-acid sequences of FEHs from other plants. The <it>aleh1 </it>encoded a polypeptide of 581 amino acids. The relative molecular mass and isoelectric point (<it>pI</it>) of the deduced polypeptide were calculated to be 65,666 and 4.86. A recombinant protein of <it>aleh1 </it>was produced in <it>Pichia pastoris</it>, and was purified by ion exchange chromatography with DEAE-Sepharose CL-6B, hydrophobic chromatography with Toyopearl HW55S and gel filtration chromatography with Toyopearl HW55S. Purified recombinant protein showed hydrolyzing activity against β-2, 1 type fructans such as 1-kestose, nystose, fructosylnystose and inulin. On the other hand, sucrose, neokestose, 6-kestose and high DP levan were poor substrates.</p> <p>The purified recombinant protein released fructose from sugars extracted from burdock roots. These results indicated that <it>aleh1 </it>encoded 1-FEH.</p

    Studies of the Electrical Parameters and Behavioral Effects of an EEG \u27\u27Seizure Discharge

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    Nemastrina monkeys with multiple electrodes implanted in the amygdaloid area of the brain were used to study the relationship of electrical seizures and overt behavior. The electrical seizures were produced by long duration electrical pulses administered through the implanted electrodes. There appears to be no relationship between this kind of electrical seizure and lever pressing performance. Relationships between depth EEG, depth impedance, DC potential and surface EEG are observed and as a consequence some ideas concerning the genesis of the electroencephalogram are put forth
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